LOCUS       40924_49512        3536 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector YDp-W, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Stolz J.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2002) Cell Biology and Plant Physiology, 
            Regensburg University, Universitaetsstr. 31, Regensburg D-93040, 
            Germany
REFERENCE   2  (bases 1 to 3536)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3536)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (30-SEP-2002) Cell Biology and Plant Physiology, Regensburg 
            University, Universitaetsstr. 31, Regensburg D-93040, Germany"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3536
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        37..138
                     /label=TRP1 promoter
     CDS             139..810
                     /codon_start=1
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     primer_bind     complement(920..936)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(944..960)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(968..998)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1013..1034)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1322..1910)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2084..2941)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2942..3046)
                     /label=AmpR promoter
     primer_bind     3520..3536
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcccgg ggatccggtg attgattgag caagctaatt cggtcgaaaa aagaaaagga
       61 gagggccaag agggagggca ttggtgacta ttgagcacgt gagtatacgt gattaagcac
      121 acaaaggcag cttggagtat gtctgttatt aatttcacag gtagttctgg tccattggtg
      181 aaagtttgcg gcttgcagag cacagaggcc gcagaatgtg ctctagattc cgatgctgac
      241 ttgctgggta ttatatgtgt gcccaataga aagagaacaa ttgacccggt tattgcaagg
      301 aaaatttcaa gtcttgtaaa agcatataaa aatagttcag gcactccgaa atacttggtt
      361 ggcgtgtttc gtaatcaacc taaggaggat gttttggctc tggtcaatga ttacggcatt
      421 gatatcgtcc aactgcatgg agatgagtcg tggcaagaat accaagagtt cctcggtttg
      481 ccagttatta aaagactcgt atttccaaaa gactgcaaca tactactcag tgcagcttca
      541 cagaaacctc attcgtttat tcccttgttt gattcagaag caggtgggac aggtgaactt
      601 ttggattgga actcgatttc tgactgggtt ggaaggcaag agagccccga aagcttacat
      661 tttatgttag ctggtggact gacgccagaa aatgttggtg atgcgcttag attaaatggc
      721 gttattggtg ttgatgtaag cggaggtgtg gagacaaatg gtgtaaaaga ctctaacaaa
      781 atagcaaatt tcgtcaaaaa tgctaagaaa taggttatta ctgagtagta tttatttaag
      841 tattgtttgt gcacttgccc ttgctcaatc aatcaccgga tccgtcgacc tgcagccaag
      901 ctagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
      961 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     1021 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     1081 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     1141 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     1201 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     1261 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     1321 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     1381 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1441 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1501 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1561 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1621 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1681 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     1741 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     1801 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     1861 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     1921 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     1981 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     2041 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     2101 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     2161 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     2221 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     2281 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     2341 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     2401 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     2461 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     2521 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     2581 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     2641 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     2701 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     2761 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     2821 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     2881 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     2941 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     3001 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     3061 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     3121 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     3181 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     3241 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     3301 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     3361 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     3421 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     3481 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagt
//
