YDp-H vector (V001499)

MSDS

Basic Vector Information

- - Vector Name:
  - YDp-H
- - Antibiotic Resistance:
  - Ampicillin
- - Length:
  - 3868 bp
- - Type:
  - Cloning vector
- - Replication origin:
  - ori
- - Source/Author:
  - Stolz J.
- - Promoter:
  - HIS3

YDp-H vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

YDp-H vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_49492        3868 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector YDp-H, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Stolz J.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2002) Cell Biology and Plant Physiology, 
            Regensburg University, Universitaetsstr. 31, Regensburg D-93040, 
            Germany
REFERENCE   2  (bases 1 to 3868)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3868)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (30-SEP-2002) Cell Biology and Plant Physiology, Regensburg 
            University, Universitaetsstr. 31, Regensburg D-93040, Germany"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        138..325
                     /label=HIS3 promoter
     CDS             326..985
                     /codon_start=1
                     /label=HIS3
                     /note="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     primer_bind     complement(1252..1268)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1276..1292)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1300..1330)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1345..1366)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1654..2242)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2416..3273)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3274..3378)
                     /label=AmpR promoter
     primer_bind     3852..3868
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"

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