Basic Vector Information
YDp-H vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
YDp-H vector Sequence
LOCUS 40924_49492 3868 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector YDp-H, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3868) AUTHORS Stolz J. TITLE Direct Submission JOURNAL Submitted (30-SEP-2002) Cell Biology and Plant Physiology, Regensburg University, Universitaetsstr. 31, Regensburg D-93040, Germany REFERENCE 2 (bases 1 to 3868) TITLE Direct Submission REFERENCE 3 (bases 1 to 3868) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted (30-SEP-2002) Cell Biology and Plant Physiology, Regensburg University, Universitaetsstr. 31, Regensburg D-93040, Germany" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..3868 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 138..325 /label=HIS3 promoter CDS 326..985 /codon_start=1 /label=HIS3 /note="imidazoleglycerol-phosphate dehydratase, required for histidine biosynthesis" /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM" primer_bind complement(1252..1268) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(1276..1292) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1300..1330) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(1345..1366) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(1654..2242) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2416..3273) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(3274..3378) /label=AmpR promoter primer_bind 3852..3868 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants"
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