LOCUS       40924_49492        3868 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector YDp-H, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Stolz J.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2002) Cell Biology and Plant Physiology, 
            Regensburg University, Universitaetsstr. 31, Regensburg D-93040, 
            Germany
REFERENCE   2  (bases 1 to 3868)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3868)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (30-SEP-2002) Cell Biology and Plant Physiology, Regensburg 
            University, Universitaetsstr. 31, Regensburg D-93040, Germany"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        138..325
                     /label=HIS3 promoter
     CDS             326..985
                     /codon_start=1
                     /label=HIS3
                     /note="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     primer_bind     complement(1252..1268)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1276..1292)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1300..1330)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1345..1366)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1654..2242)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2416..3273)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3274..3378)
                     /label=AmpR promoter
     primer_bind     3852..3868
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcccgg ggatccggtg attgattgag caagcttagg agtcactgcc aggtatcgtt
       61 tgaacacggc attagtcagg gaagtcataa cacagtcctt tcccgcaatt ttctttttct
      121 attactcttg gcctcctcta gtacactcta tattttttta tgcctcggta atgattttca
      181 tttttttttt tccacctagc ggatgactct ttttttttct tagcgattgg cattatcaca
      241 taatgaatta tacattatat aaagtaatgt gatttcttcg aagaatatac taaaaaatga
      301 gcaggcaaga taaacgaagg caaagatgac agagcagaaa gccctagtaa agcgtattac
      361 aaatgaaacc aagattcaga ttgcgatctc tttaaagggt ggtcccctag cgatagagca
      421 ctcgatcttc ccagaaaaag aggcagaagc agtagcagaa caggccacac aatcgcaagt
      481 gattaacgtc cacacaggta tagggtttct ggaccatatg atacatgctc tggccaagca
      541 ttccggctgg tcgctaatcg ttgagtgcat tggtgactta cacatagacg accatcacac
      601 cactgaagac tgcgggattg ctctcggtca agcttttaaa gaggccctag gggccgtgcg
      661 tggagtaaaa aggtttggat caggatttgc gcctttggat gaggcacttt ccagagcggt
      721 ggtagatctt tcgaacaggc cgtacgcagt tgtcgaactt ggtttgcaaa gggagaaagt
      781 aggagatctc tcttgcgaga tgatcccgca ttttcttgaa agctttgcag aggctagcag
      841 aattaccctc cacgttgatt gtctgcgagg caagaatgat catcaccgta gtgagagtgc
      901 gttcaaggct cttgcggttg ccataagaga agccacctcg cccaatggta ccaacgatgt
      961 tccctccacc aaaggtgttc ttatgtagtg acaccgatta tttaaagctg cagcatacga
     1021 tatatataca tgtgtatata tgtataccta tgaatgtcag taagtatgta tacgaacagt
     1081 atgatactga agatgacaag gtaatgcatc attctatacg tgtcattctg aacgaggcgc
     1141 gctttccttt tttctttttg ctttttcttt ttttttctct tgaactcgaa gcttgctcaa
     1201 tcaatcaccg gatccgtcga cctgcagcca agctagcttg gcgtaatcat ggtcatagct
     1261 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     1321 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     1381 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     1441 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     1501 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     1561 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     1621 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     1681 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     1741 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     1801 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     1861 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1921 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1981 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     2041 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     2101 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     2161 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     2221 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     2281 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     2341 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     2401 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     2461 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     2521 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     2581 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     2641 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     2701 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     2761 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     2821 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     2881 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     2941 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     3001 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     3061 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     3121 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     3181 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     3241 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     3301 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     3361 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     3421 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
     3481 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     3541 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     3601 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     3661 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc
     3721 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     3781 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     3841 gtcacgacgt tgtaaaacga cggccagt
//
