LOCUS 40924_49492 3868 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector YDp-H, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3868) AUTHORS Stolz J. TITLE Direct Submission JOURNAL Submitted (30-SEP-2002) Cell Biology and Plant Physiology, Regensburg University, Universitaetsstr. 31, Regensburg D-93040, Germany REFERENCE 2 (bases 1 to 3868) TITLE Direct Submission REFERENCE 3 (bases 1 to 3868) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted (30-SEP-2002) Cell Biology and Plant Physiology, Regensburg University, Universitaetsstr. 31, Regensburg D-93040, Germany" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..3868 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 138..325 /label=HIS3 promoter CDS 326..985 /codon_start=1 /label=HIS3 /note="imidazoleglycerol-phosphate dehydratase, required for histidine biosynthesis" /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM" primer_bind complement(1252..1268) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(1276..1292) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1300..1330) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(1345..1366) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(1654..2242) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2416..3273) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(3274..3378) /label=AmpR promoter primer_bind 3852..3868 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 gaattcccgg ggatccggtg attgattgag caagcttagg agtcactgcc aggtatcgtt 61 tgaacacggc attagtcagg gaagtcataa cacagtcctt tcccgcaatt ttctttttct 121 attactcttg gcctcctcta gtacactcta tattttttta tgcctcggta atgattttca 181 tttttttttt tccacctagc ggatgactct ttttttttct tagcgattgg cattatcaca 241 taatgaatta tacattatat aaagtaatgt gatttcttcg aagaatatac taaaaaatga 301 gcaggcaaga taaacgaagg caaagatgac agagcagaaa gccctagtaa agcgtattac 361 aaatgaaacc aagattcaga ttgcgatctc tttaaagggt ggtcccctag cgatagagca 421 ctcgatcttc ccagaaaaag aggcagaagc agtagcagaa caggccacac aatcgcaagt 481 gattaacgtc cacacaggta tagggtttct ggaccatatg atacatgctc tggccaagca 541 ttccggctgg tcgctaatcg ttgagtgcat tggtgactta cacatagacg accatcacac 601 cactgaagac tgcgggattg ctctcggtca agcttttaaa gaggccctag gggccgtgcg 661 tggagtaaaa aggtttggat caggatttgc gcctttggat gaggcacttt ccagagcggt 721 ggtagatctt tcgaacaggc cgtacgcagt tgtcgaactt ggtttgcaaa gggagaaagt 781 aggagatctc tcttgcgaga tgatcccgca ttttcttgaa agctttgcag aggctagcag 841 aattaccctc cacgttgatt gtctgcgagg caagaatgat catcaccgta gtgagagtgc 901 gttcaaggct cttgcggttg ccataagaga agccacctcg cccaatggta ccaacgatgt 961 tccctccacc aaaggtgttc ttatgtagtg acaccgatta tttaaagctg cagcatacga 1021 tatatataca tgtgtatata tgtataccta tgaatgtcag taagtatgta tacgaacagt 1081 atgatactga agatgacaag gtaatgcatc attctatacg tgtcattctg aacgaggcgc 1141 gctttccttt tttctttttg ctttttcttt ttttttctct tgaactcgaa gcttgctcaa 1201 tcaatcaccg gatccgtcga cctgcagcca agctagcttg gcgtaatcat ggtcatagct 1261 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 1321 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 1381 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 1441 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 1501 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 1561 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 1621 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 1681 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 1741 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 1801 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 1861 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 1921 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 1981 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 2041 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 2101 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 2161 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 2221 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 2281 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 2341 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 2401 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 2461 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 2521 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 2581 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 2641 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 2701 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 2761 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 2821 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 2881 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 2941 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 3001 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 3061 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 3121 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 3181 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 3241 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 3301 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 3361 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat 3421 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg 3481 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 3541 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 3601 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat 3661 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc 3721 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca 3781 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca 3841 gtcacgacgt tgtaaaacga cggccagt //