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YCp85 vector (V001503#)

Basic Vector Information

Basic Vector Information
Vector Name YCp85 Antibiotic Resistance Ampicillin
Length 4945 bp Type Centromeric vector
Source Baruffini E, Serafini F, Lodi T.

YCp85 vector Vector Map

lacZ' lac operator lac promoter CAP binding site TEF-promoter TEF-terminator ARS-CEN6 ori ampR AmpR promoter

YCp85 vector Sequence

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Download GeneBank File(.gb)

LOCUS       Exported                4945 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Centromeric vector YCp85, complete sequence.
ACCESSION   FJ834446
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Baruffini E, Serafini F, Lodi T.
  TITLE     Construction and characterization of centromeric, episomal and 
            GFP-containing vectors for Saccharomyces cerevisiae prototrophic 
            strains
  JOURNAL   J. Biotechnol. 143 (4), 247-254 (2009)
  PUBMED    19683551
REFERENCE   2  (bases 1 to 4945)
  AUTHORS   Baruffini E, Serafini F, Lodi T.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2009) Department of Genetics, Biology of 
            Microorganisms, Anthropology, Evolution, University of Parma, Viale 
            Usberti 11/A, Parma 43100, Italy
REFERENCE   3  (bases 1 to 4945)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Centromeric vector YCp85"
                     /lab_host="Saccharomyces cerevisiae"
                     /mol_type="other DNA"
                     /db_xref="taxon:670501"
     CDS             complement(146..469)
                     /codon_start=1
                     /gene="lacZ'"
                     /product="LacZ'"
                     /label=lacZ'
                     /note="beta-galactosidase alpha"
                     /protein_id="ACV50009.1"
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     gene            complement(146..469)
                     /gene="lacZ'"
                     /label=lacZ'
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    396..452
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     misc_feature    397..446
                     /note="multiple cloning site; MCS"
     primer_bind     complement(465..481)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    489..505
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(513..543)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    558..579
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     regulatory      632..1020
                     /regulatory_class="promoter"
                     /note="TEF-promoter"
     gene            677..2033
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        677..1020
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1021..1830
                     /codon_start=1
                     /gene="kanR"
                     /product="KanR"
                     /label=kanR
                     /note="aminoglycoside 3'-phosphotransferase; APH"
                     /protein_id="ACV50007.1"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            1021..1830
                     /gene="kanR"
                     /label=kanR
     regulatory      1831..2042
                     /regulatory_class="terminator"
                     /note="TEF-terminator"
     terminator      1836..2033
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     rep_origin      2190..3010
                     /label=ARS-CEN6
                     /note="ARS-CEN6"
     rep_origin      complement(3126..3714)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3885..4745)
                     /codon_start=1
                     /gene="ampR"
                     /product="AmpR"
                     /label=ampR
                     /note="beta-lactamase"
                     /protein_id="ACV50008.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(3885..4745)
                     /gene="ampR"
                     /label=ampR
     promoter        complement(4746..4850)
                     /gene="bla"
                     /label=AmpR promoter

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