LOCUS       40924_49457        4945 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Centromeric vector YCp85, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Baruffini E, Serafini F, Lodi T.
  TITLE     Construction and characterization of centromeric, episomal and 
            GFP-containing vectors for Saccharomyces cerevisiae prototrophic 
            strains
  JOURNAL   J. Biotechnol. 143 (4), 247-254 (2009)
  PUBMED    19683551
REFERENCE   2  (bases 1 to 4945)
  AUTHORS   Baruffini E, Serafini F, Lodi T.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2009) Department of Genetics, Biology of 
            Microorganisms, Anthropology, Evolution, University of Parma, Viale 
            Usberti 11/A, Parma 43100, Italy
REFERENCE   3  (bases 1 to 4945)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4945)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Biotechnol."; date: "2009"; volume: "143"; issue: "4"; pages: 
            "247-254"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-MAR-2009) Department of Genetics, Biology of Microorganisms, 
            Anthropology, Evolution, University of Parma, Viale Usberti 11/A, 
            Parma 43100, Italy"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    396..452
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(465..481)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(489..505)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(513..543)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(558..579)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     gene            677..2033
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     rep_origin      2190..3010
                     /label=ARS-CEN6
                     /note="ARS-CEN6"
     rep_origin      complement(3126..3714)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3888..4745)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4746..4850)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
      421 cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata gctgtttcct
      481 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      541 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      601 gctttccagt cgggaaacct gtcgtgccag cagatctgtt tagcttgcct cgtccccgcc
      661 gggtcacccg gccagcgaca tggaggccca gaataccctc cttgacagtc ttgacgtgcg
      721 cagctcaggg gcatgatgtg actgtcgccc gtacatttag cccatacatc cccatgtata
      781 atcatttgca tccatacatt ttgatggccg cacggcgcga agcaaaaatt acggctcctc
      841 gctgcagacc tgcgagcagg gaaacgctcc cctcacagac gcgttgaatt gtccccacgc
      901 cgcgcccctg tagagaaata taaaaggtta ggatttgcca ctgaggttct tctttcatat
      961 acttcctttt aaaatcttgc taggatacag ttctcacatc acatccgaac ataaacaacc
     1021 atgggtaagg aaaagactca cgtttcgagg ccgcgattaa attccaacat ggatgctgat
     1081 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga
     1141 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
     1201 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg
     1261 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc
     1321 ggcaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
     1381 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac
     1441 agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat
     1501 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
     1561 cataagcttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
     1621 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc
     1681 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
     1741 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
     1801 tttcatttga tgctcgatga gtttttctaa tcagtactga caataaaaag attcttgttt
     1861 tcaagaactt gtcatttgta tagttttttt atattgtagt tgttctattt taatcaaatg
     1921 ttagcgtgat ttatattttt tttcgcctcg acatcatctg cccagatgcg aagttaagtg
     1981 cgcagaaagt aatatcatgc gtcaatcgta tgtgaatgct ggtcgctata ctgctgtcga
     2041 ttcgatacta acgccgccat ccagatctgc tgcattaatg aatcggccaa cgcgcgggga
     2101 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     2161 tcgttcggct gcggcgagcg gtatcagcat cgatgaatgg aaattacgta ctttcaacgc
     2221 aagagcaaga caaaagaaag cctaaaactg tcttttcgtg tcggtcgtcc aatatcatcg
     2281 taaacgtgtg tagagcctta aattctaatt tctaacttta aatttttttt gctattataa
     2341 tactaatttc taactttcaa cctattttac atcttcggaa aacaaaaact attttttctt
     2401 taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa atttaaatta
     2461 tattattttt atagcacgtg atgaaaagaa atatagtctt caaggttata ttatacaggt
     2521 ttatatatta ttttacttat ttttgttgtt ctttttcttg gagtaattta gcattaacaa
     2581 cttcgaccca tcgcgatgac gaaattgcac gtttaaagga gaagtcggaa tcgttgcaag
     2641 aagaaataaa ttagtttgac gttttattcc ttttcttctg cacagaagtg tgtatatata
     2701 tatttatata tagtcgatgt gcaaaagcct acattatttt tcttttgatt tttatttttt
     2761 tgttcaagta ggttcatgcc gacagtaaat ccttatgccc tacctaaata taaacaccca
     2821 aatatatata tatatatata tatatataaa taataaaaac ggaaaggaaa tagaataata
     2881 ataataatac tacacgttta cgtatgtaac tcattctatt attcagactt tgatattaat
     2941 agaattatta ctactaggtt tcctcacttc aaaatctata aatataccgt catgctctaa
     3001 tcgatgctca ctcaaaggtc ggtaatacgg ttatccacag aatcagggga taacgcagga
     3061 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     3121 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     3181 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     3241 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     3301 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     3361 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     3421 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3481 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3541 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     3601 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3661 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3721 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     3781 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     3841 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     3901 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     3961 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     4021 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     4081 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     4141 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     4201 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     4261 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     4321 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     4381 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     4441 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     4501 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     4561 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     4621 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     4681 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     4741 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     4801 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     4861 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     4921 aggcgtatca cgaggccctt tcgtc
//
