WRKY29-LUC-NOS-At4g23550 vector (V001511)

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LOCUS       40924_49377        7292 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Control vector WRKY29-LUC-NOS-At4g23550, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7292)
  AUTHORS   Asai T, Tena G, Plotnikova J, Willmann MR, Chiu WL, Gomez-Gomez L, 
            Boller T, Ausubel FM, Sheen J.
  TITLE     MAP kinase signalling cascade in Arabidopsis innate immunity
  JOURNAL   Nature 415 (6875), 977-983 (2002)
  PUBMED    11875555
REFERENCE   2  (bases 1 to 7292)
  AUTHORS   Sheen J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2006) Molecular Biology, Massachusetts General 
            Hospital, 185 Cambridge St. Simches Research Building-CPZN7600, 
            Boston, MA 02114, USA
REFERENCE   3  (bases 1 to 7292)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7292)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature"; 
            date: "2002"; volume: "415"; issue: "6875"; pages: "977-983"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-OCT-2006) Molecular Biology, Massachusetts General Hospital, 185
            Cambridge St. Simches Research Building-CPZN7600, Boston, MA 02114, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(15..31)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(39..55)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(63..93)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(108..129)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(417..1005)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1179..2036)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2037..2141)
                     /label=AmpR promoter
     primer_bind     2615..2631
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2662..5228
                     /label=pWRKY29
                     /note="pWRKY29"
     regulatory      2667..5228
                     /label=WRKY29 promoter
                     /note="WRKY29 promoter"
                     /regulatory_class="promoter"
     CDS             5229..6878
                     /label=luciferase
                     /note="firefly luciferase"
     terminator      7036..7287
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"