LOCUS       40924_49377        7292 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Control vector WRKY29-LUC-NOS-At4g23550, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7292)
  AUTHORS   Asai T, Tena G, Plotnikova J, Willmann MR, Chiu WL, Gomez-Gomez L, 
            Boller T, Ausubel FM, Sheen J.
  TITLE     MAP kinase signalling cascade in Arabidopsis innate immunity
  JOURNAL   Nature 415 (6875), 977-983 (2002)
  PUBMED    11875555
REFERENCE   2  (bases 1 to 7292)
  AUTHORS   Sheen J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2006) Molecular Biology, Massachusetts General 
            Hospital, 185 Cambridge St. Simches Research Building-CPZN7600, 
            Boston, MA 02114, USA
REFERENCE   3  (bases 1 to 7292)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7292)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature"; 
            date: "2002"; volume: "415"; issue: "6875"; pages: "977-983"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-OCT-2006) Molecular Biology, Massachusetts General Hospital, 185
            Cambridge St. Simches Research Building-CPZN7600, Boston, MA 02114, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(15..31)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(39..55)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(63..93)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(108..129)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(417..1005)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1179..2036)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2037..2141)
                     /label=AmpR promoter
     primer_bind     2615..2631
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2662..5228
                     /label=pWRKY29
                     /note="pWRKY29"
     regulatory      2667..5228
                     /label=WRKY29 promoter
                     /note="WRKY29 promoter"
                     /regulatory_class="promoter"
     CDS             5229..6878
                     /label=luciferase
                     /note="firefly luciferase"
     terminator      7036..7287
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
       61 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
      121 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
      181 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
      241 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      301 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      361 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      421 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      481 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      541 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      601 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      661 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      721 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      781 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      841 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      901 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      961 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     1021 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     1081 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     1141 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     1201 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1261 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     1321 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1381 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1441 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1501 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1561 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1621 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1681 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     1741 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     1801 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1861 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1921 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     1981 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     2041 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     2101 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     2161 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     2221 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     2281 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     2341 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     2401 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     2461 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     2521 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     2581 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctt
     2641 gcatgcctgc aggctctaga ggatcctcgt ccatgatgga catatatgtg actgcgtatt
     2701 tacacacacc gcacgtatgc ttatttcaca cgttagaaga agaattcaaa gaagtcggtt
     2761 ctatttgtat tcttgtgaga tcatcaatat gacaatatcg ttctattaat atacgtataa
     2821 ttcatatgtt gtcatggttt cacataccat gtcgacagtc gacgtacgta caaaagtata
     2881 aatagtatga atctaataac agcaccaaga ttgaagttca tcttctaatc aaaactatca
     2941 taaagtggtt tcaaaatagt gttttttctg atgaaactat aactgagtta taatcaatcc
     3001 gaaattatat aactaattat atttgggaac tagataaacg caaaaacatg agcagtttct
     3061 tatttttttt gtccagattt aaaatttgga gtgttaaaat atacggagtg ttacacaatg
     3121 aaaacacaag aagtcaagaa cccataagtt attttaatta ataatattgt atttaaagtg
     3181 attattaaaa aataatgtaa aaactgatta tttgttgaca aaaaacagtt agttatagtt
     3241 aaatagtatt gatgcatata tatactatct cattattttg gtattactca gtactcacat
     3301 ctttaataaa gacaaagata gttagtgtat aattcaaatc gaactcacag aagtcaataa
     3361 gcgcgtaaaa atacaaaaat atctggcaga ctttagcaag gtttgttttc caacagaaat
     3421 ggtcatttca gaatcattta catatccata tatatagctc ttaaatggta tatattgggt
     3481 aatgggtatt cgtttaaata attttgtttt ctgtaaattt caaatattaa tctgatcagt
     3541 ttatccatgt gtgtatattt agtgtattat catcaatata tgacatagac agactttcaa
     3601 gttggtgcaa gaggggatga aaatttcttc ccagttgcaa gagtaagctg actagcattt
     3661 tttttttata tataatttat ttctcaaatg gtttttatta ttgttttgtt gactttaagt
     3721 ttttgccttt tatgggactg caatcacccg tgccaacttt taatctccat cgcctaaaaa
     3781 agaaagaaaa ggctaccatt atggaccgaa atatttaaga ccataataca aaattatacg
     3841 aatattttct gtaacttata tatatgatca tttgacaaag caaagtcaat caaataaact
     3901 tcaaagaaat tatgagctta taataagttt gatagtgtta aatataatca aatcattaaa
     3961 tttagtattt atttcacctc ggtttccatt tgatagatag ataaatgaaa gatagcatcg
     4021 ccaataatga aaaaacttta tttgatggca atactttgtt acatcatttc tgttttctta
     4081 atttcatgtc gaaatattgc catgattgtg ttcaacataa ctagttttga ggtaacaagt
     4141 taaaaatttg ttatattttt tgaatatgtt attcagttga aagtcattta gatgtaagta
     4201 aaaacaaaca taagaagtta acatatcaat attaacacag cgaataatca ttattacaaa
     4261 aaaaagcaaa aaaatagaag aagatattat atattggaga atctttcttt agtcttagtt
     4321 gggaagattt tgtgctatgg gattaaaggt atccatccct attctatgat agaggcgtgg
     4381 ggttattgga ccaatctata tatattacca caaggcttaa gatgaagtga taatacagta
     4441 ttattaatac cctcccaaat tatttttaaa tatttatcaa aagaagctta cggtatagat
     4501 catacttgca gcattattct ataagtttat ttaatttcag tggctcgtta cgtgaacaca
     4561 aggtaagcta atagacttac gtgccccatt aaacacatac ataattatac aagtatcatg
     4621 aaactagtga caaaacctcg atcaaataaa gaaattacca tgacgacaaa agataattaa
     4681 aaaaaaaact actatatgtc atactcatgc atatgcatgt acaaatgccg ctttaaatat
     4741 ttaatttagt taaagcaatg atatttaaat tctctctact tcatatatat tccaaaagac
     4801 atattgtcaa attccttctt ttagttatat ataatcatat attcatattg ttatattttc
     4861 aatatttaat agtaagatgg actttcctga atgttgtgta tgatttataa tttgagatat
     4921 tttgtcggag atggatattt gacaagttaa tgttacttta ttaaaatttt ctaaacattt
     4981 aggtacgaat tgactttttc aaaagtcaac acaataaatt ttaaaagttt aatgacttaa
     5041 cgggttcaca tgggaaacga aaacacccta aaccacaaac aatctaatct tatttccttc
     5101 tttatataaa ccgctgtttc ccaaaaggct tgttctcgtc atatgtactt gtacaccaac
     5161 ccaccaaaag agataaaaga ggaaacaaaa actcgaaaag agagagatat atgggtgagg
     5221 tggcttccat ggaagacgcc aaaaacataa agaaaggccc ggcgccattc tatcctctag
     5281 aggatggaac cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg
     5341 gaacaattgc ttttacagat gcacatatcg aggtgaacat cacgtacgcg gaatacttcg
     5401 aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa
     5461 tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta
     5521 tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta
     5581 tgaacatttc gcagcctacc gtagtgtttg tttccaaaaa ggggttgcaa aaaattttga
     5641 acgtgcaaaa aaaattacca ataatccaga aaattattat catggattct aaaacggatt
     5701 accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg
     5761 aatacgattt tgtaccagag tcctttgatc gtgacaaaac aattgcactg ataatgaatt
     5821 cctctggatc tactgggtta cctaagggtg tggcccttcc gcatagaact gcctgcgtca
     5881 gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt
     5941 taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat
     6001 gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgttttta cgatcccttc
     6061 aggattacaa aattcaaagt gcgttgctag taccaaccct attttcattc ttcgccaaaa
     6121 gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct gggggcgcac
     6181 ctctttcgaa agaagtcggg gaagcggttg caaaacgctt ccatcttcca gggatacgac
     6241 aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag ggggatgata
     6301 aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata
     6361 ccgggaaaac gctgggcgtt aatcagagag gcgaattatg tgtcagagga cctatgatta
     6421 tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc
     6481 tacattctgg agacatagct tactgggacg aagacgaaca cttcttcata gttgaccgct
     6541 tgaagtcttt aattaaatac aaaggatatc aggtggcccc cgctgaattg gaatcgatat
     6601 tgttacaaca ccccaacatc ttcgacgcgg gcgtggcagg tcttcccgac gatgacgccg
     6661 gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga
     6721 tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt
     6781 ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga
     6841 tcctcataaa ggccaagaag ggcggaaagt ccaaattgta aaatgtaact gtattcagcg
     6901 atgacgaaat tcttagctat tgtaatatta tatgcaaatt gatgaatggt aattttgtaa
     6961 ttgtgggtca ctgtactatt ttaacgaata ataaaatcag gtataggtaa ctaaaaagga
     7021 attcgagctc gaattgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct
     7081 gttgccggtc ttgcgatgat tatcatataa tttctgttga ttacgttaag catgtaataa
     7141 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
     7201 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
     7261 gcgcggtgtc atctatgtta ctagatctct ag
//
