UASp vector (V001583)

Basic Vector Information

Vector Name:
UASp
Antibiotic Resistance:
Ampicillin
Length:
9939 bp
Type:
P-element transposon vector
Replication origin:
ori
Source/Author:
Miller DFB., Kaufman TC.

UASp vector Map

UASp9939 bp4008001200160020002400280032003600400044004800520056006000640068007200760080008400880092009600contains 2 GAGA sitescontains 14 UAS Gal4 binding sitescontains P-transposase promoterK10 polyA signal sequenceP element 3' endAmpR promoterAmpRoriP element 5' endmini-white

UASp vector Sequence

LOCUS       40924_48992        9939 bp DNA     circular SYN 18-DEC-2018
DEFINITION  P-element transposon vector UASp, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9939)
  AUTHORS   Miller DFB., Kaufman TC.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2004) Biology, Indiana University, 1001 E. Third 
            St., Bloomington, IN 47405, USA
REFERENCE   2  (bases 1 to 9939)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9939)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (19-NOV-2004) Biology, Indiana University, 1001 E. Third St., 
            Bloomington, IN 47405, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9939
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..62
                     /label=contains 2 GAGA sites
                     /note="contains 2 GAGA sites"
     misc_feature    99..445
                     /label=contains 14 UAS Gal4 binding sites
                     /note="contains 14 UAS Gal4 binding sites"
     regulatory      457..701
                     /label=contains P-transposase promoter
                     /note="contains P-transposase promoter"
                     /regulatory_class="promoter"
     misc_feature    683..745
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    795..2220
                     /label=K10 polyA signal sequence
                     /note="K10 polyA signal sequence"
     misc_feature    2229..2461
                     /label=P element 3' end
                     /note="P element 3' end"
     promoter        3265..3369
                     /label=AmpR promoter
     CDS             3370..4227
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4401..4989
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    5224..5809
                     /label=P element 5' end
     gene            complement(5820..9939)
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."

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