UASp vector (V001583)

Basic Vector Information

      • Vector Name:
      • UASp
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 9939 bp
      • Type:
      • P-element transposon vector
      • Source/Author:
      • Miller DFB., Kaufman TC.

UASp vector Vector Map

UASp9939 bp4008001200160020002400280032003600400044004800520056006000640068007200760080008400880092009600contains 2 GAGA sitescontains 14 UAS Gal4 binding sitescontains P-transposase promoterK10 polyA signal sequence3' signal sequencederived from pUC8P element 5' endwhite

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

UASp vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                9939 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  P-element transposon vector UASp, complete sequence.
ACCESSION   AY831681
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9939)
  AUTHORS   Miller DFB., Kaufman TC.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2004) Biology, Indiana University, 1001 E. Third 
            St., Bloomington, IN 47405, USA
REFERENCE   2  (bases 1 to 9939)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9939
                     /organism="P-element transposon vector UASp"
                     /mol_type="other DNA"
                     /note="constructed by P. Rorth from pCasper4 to enhance 
                     Gal4 directed transcription in the germline, Mech. Dev. 78,
                     113-118 (1998)"
                     /db_xref="taxon:304772"
     misc_feature    1..62
                     /label=contains 2 GAGA sites
                     /note="contains 2 GAGA sites"
     misc_feature    99..445
                     /label=contains 14 UAS Gal4 binding sites
                     /note="contains 14 UAS Gal4 binding sites"
     regulatory      457..701
                     /regulatory_class="promoter"
                     /note="contains P-transposase promoter"
     misc_feature    683..745
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    795..2220
                     /label=K10 polyA signal sequence
                     /note="K10 polyA signal sequence"
     mobile_element  complement(2229..2461)
                     /mobile_element_type="transposon:P-element"
                     /note="3' signal sequence"
     misc_feature    2229..2461
                     /label=P element 3' end
                     /note="P element 3' end"
     misc_feature    2965..4993
                     /label=derived from pUC8
                     /note="derived from pUC8"
     promoter        3265..3369
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3370..4230
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     mobile_element  complement(3809..5224)
                     /mobile_element_type="transposon:P-element"
                     /note="5' signal sequence"
     rep_origin      4401..4989
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    5224..5809
                     /label=P element 5' end
     CDS             complement(join(6588..7202,7273..7404,7608..7923,7985..8639,
                     8714..8987,9389..9460))
                     /codon_start=1
                     /product="white"
                     /label=white
                     /protein_id="AAV97853.1"
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"

This page is informational only.