UASp vector (V001583)

Basic Vector Information

Vector Name:
UASp
Antibiotic Resistance:
Ampicillin
Length:
9939 bp
Type:
P-element transposon vector
Replication origin:
ori
Source/Author:
Miller DFB., Kaufman TC.

UASp vector Vector Map

UASp9939 bp4008001200160020002400280032003600400044004800520056006000640068007200760080008400880092009600contains 2 GAGA sitescontains 14 UAS Gal4 binding sitescontains P-transposase promoterK10 polyA signal sequenceP element 3' endAmpR promoterAmpRoriP element 5' endmini-white

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

UASp vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_48992        9939 bp DNA     circular SYN 18-DEC-2018
DEFINITION  P-element transposon vector UASp, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9939)
  AUTHORS   Miller DFB., Kaufman TC.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2004) Biology, Indiana University, 1001 E. Third 
            St., Bloomington, IN 47405, USA
REFERENCE   2  (bases 1 to 9939)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9939)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (19-NOV-2004) Biology, Indiana University, 1001 E. Third St., 
            Bloomington, IN 47405, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9939
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..62
                     /label=contains 2 GAGA sites
                     /note="contains 2 GAGA sites"
     misc_feature    99..445
                     /label=contains 14 UAS Gal4 binding sites
                     /note="contains 14 UAS Gal4 binding sites"
     regulatory      457..701
                     /label=contains P-transposase promoter
                     /note="contains P-transposase promoter"
                     /regulatory_class="promoter"
     misc_feature    683..745
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    795..2220
                     /label=K10 polyA signal sequence
                     /note="K10 polyA signal sequence"
     misc_feature    2229..2461
                     /label=P element 3' end
                     /note="P element 3' end"
     promoter        3265..3369
                     /label=AmpR promoter
     CDS             3370..4227
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4401..4989
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    5224..5809
                     /label=P element 5' end
     gene            complement(5820..9939)
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."

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