Basic Vector Information
UASp vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
UASp vector Sequence
LOCUS 40924_48992 9939 bp DNA circular SYN 18-DEC-2018 DEFINITION P-element transposon vector UASp, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9939) AUTHORS Miller DFB., Kaufman TC. TITLE Direct Submission JOURNAL Submitted (19-NOV-2004) Biology, Indiana University, 1001 E. Third St., Bloomington, IN 47405, USA REFERENCE 2 (bases 1 to 9939) TITLE Direct Submission REFERENCE 3 (bases 1 to 9939) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted (19-NOV-2004) Biology, Indiana University, 1001 E. Third St., Bloomington, IN 47405, USA" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..9939 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 1..62 /label=contains 2 GAGA sites /note="contains 2 GAGA sites" misc_feature 99..445 /label=contains 14 UAS Gal4 binding sites /note="contains 14 UAS Gal4 binding sites" regulatory 457..701 /label=contains P-transposase promoter /note="contains P-transposase promoter" /regulatory_class="promoter" misc_feature 683..745 /label=multiple cloning site /note="multiple cloning site" misc_feature 795..2220 /label=K10 polyA signal sequence /note="K10 polyA signal sequence" misc_feature 2229..2461 /label=P element 3' end /note="P element 3' end" promoter 3265..3369 /label=AmpR promoter CDS 3370..4227 /label=AmpR /note="beta-lactamase" rep_origin 4401..4989 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" misc_feature 5224..5809 /label=P element 5' end gene complement(5820..9939) /label=mini-white /note="This modified version of the white gene lacks part of the first intron."
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