LOCUS       40924_48992        9939 bp DNA     circular SYN 18-DEC-2018
DEFINITION  P-element transposon vector UASp, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9939)
  AUTHORS   Miller DFB., Kaufman TC.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2004) Biology, Indiana University, 1001 E. Third 
            St., Bloomington, IN 47405, USA
REFERENCE   2  (bases 1 to 9939)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9939)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (19-NOV-2004) Biology, Indiana University, 1001 E. Third St., 
            Bloomington, IN 47405, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9939
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..62
                     /label=contains 2 GAGA sites
                     /note="contains 2 GAGA sites"
     misc_feature    99..445
                     /label=contains 14 UAS Gal4 binding sites
                     /note="contains 14 UAS Gal4 binding sites"
     regulatory      457..701
                     /label=contains P-transposase promoter
                     /note="contains P-transposase promoter"
                     /regulatory_class="promoter"
     misc_feature    683..745
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    795..2220
                     /label=K10 polyA signal sequence
                     /note="K10 polyA signal sequence"
     misc_feature    2229..2461
                     /label=P element 3' end
                     /note="P element 3' end"
     promoter        3265..3369
                     /label=AmpR promoter
     CDS             3370..4227
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4401..4989
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    5224..5809
                     /label=P element 5' end
     gene            complement(5820..9939)
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
ORIGIN
        1 aattggccgc tctagccccc cctcgaatgt tctctctctt ctcttctctc tctctttctc
       61 gaatgttctc tctcttctct tctctctctc tttctcgagg tcatcaagct taggcctcca
      121 aggcggagta ctgtcctccg ggctggcgga gtactgtcct ccggcaaggc ggagtactgt
      181 cctccgggct ggcggagtac tgtcctccgg caaggcggag tactgtcctc cgggctggcg
      241 gagtactgtc ctccggcaag gcggagtact gtcctccggg ctggcggagt actgtcctcc
      301 ggcaaggcgg agtactgtcc tccgggctgg cggagtactg tcctccggca aggcggagta
      361 ctgtcctccg ggctggcgga gtactgtcct ccggcaaggc ggagtactgt cctccgggct
      421 ggcggagtac tgtcctccgg caagggtcga gtcgatagcc gaagcttacc gaagtataca
      481 cttaaattca gtgcacgttt gcttgttgag aggaaaggtt gtgtgcggac gaattttttt
      541 ttgaaaaccg gtgatagagc ctgaaccaga aaagataaaa gaaggctata ccagtgggag
      601 tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca ataacgtgac
      661 tgtgcgttag gtcctgttca ttggtacccg cccggggatc agatccgcgg ccgcataggc
      721 cactagtgga tctggatcct ctagagtcga cctcgaacgt taacgttaac gtaacgttaa
      781 ctcgaggagc ttgataacat tatacctaaa cccatggtca agagtaaaca tttctgcctt
      841 tgaagttgag aacacaatta agcatcccct ggttaaacct gacattcata cttgttaata
      901 gcgccataaa catagcacca attcgaagaa tacttgttaa tagcgccata aacatagcac
      961 caatttcgaa gaaactcagt taaaagcaat tagcaattag caattagcaa taactctgct
     1021 gacttcaaaa cgagaagagt tgcaagtatt tgtaaggcac agtttataga ccaccgacgg
     1081 ctcattaggg ctcgtcatgt aactaagcgc ggtgaaaccc aattgaacat atagtggaat
     1141 tattattatc aatggggaag atttaaccct caggtagcaa agtaatttaa ttgcaaatag
     1201 agagtcctaa gactaaataa tatatttaaa aatctggccc tttgaccttg cttgtcaggt
     1261 gcatttgggt tcaatcgtaa gttgcttcta tataaacact ttccccatcc ccgcaataat
     1321 gaagaatacc gcagaataaa gagagatttg caacaaaaaa taaaggcatt gcgaaaactt
     1381 tttatggggg atcattacac tcgggcctac ggttacaatt cccagccact taagcgacaa
     1441 gtttggccaa caatccatct aatagctaat agcgcaatca ctggtaatcg caagagtata
     1501 taggcaatag aacccatgga tttgaccaaa ggtaaccgag acaatggaga agcaagagga
     1561 tttcaaactg aacacccaca gtgctgtgta ctaccactgg cgcgtttggg agctcactgg
     1621 cctgatgcgt cctccgggcg tttcaagcct gctttacgtg gtatactcca ttacggtcaa
     1681 cttggtggtc accgtgctgt ttcccttgag cttgctggcc aggctgctgt tcaccaccaa
     1741 catggccgga ttgtgcgaga acctgaccat aactattacc gatattgtgg ccaatttgaa
     1801 gtttgcgaat gtgtacatgg tgaggaagca gctccatgag attcgctctc tcctaaggct
     1861 catggacgct agagcccggc tggtgggcga tcccgaggag atttctgcct tgaggaagga
     1921 agtgaatatc gcacagggca ctttccgcac ctttgccagt attttcgtat ttggcactac
     1981 tttgagttgc gtccgcgtgg tcgttcgccc ggatcgagag ctcctgtatc cggcctggtt
     2041 cggcgttgac tggatgcact ccaccagaaa ctatgtgctc atcaatatct accagctctt
     2101 cggcttgata gtgcaggcta tacagaactg cgctagtgac tcctatccgc ctgcgtttct
     2161 ctgcctgctc acgggtcata tgcgtgcttt ggagctgagg gtgcggcgga ttggctgcag
     2221 ccaagctttg cgtactcgca aattattaaa aataaaactt taaaaataat ttcgtctaat
     2281 taatattatg agttaattca aaccccacgg acatgctaag ggttaatcaa caatcatatc
     2341 gctgtctcac tcagactcaa tacgacactc agaatactat tcctttcact cgcacttatt
     2401 gcaagcatac gttaagtgga tgtctcttgc cgacgggacc accttatgtt atttcatcat
     2461 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg
     2521 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc
     2581 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg
     2641 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca
     2701 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc
     2761 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca
     2821 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg
     2881 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact
     2941 gtcgttagtt ccgcgcgatt cgggtgcggt atttcacacc gcatatggtg cactctcagt
     3001 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     3061 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     3121 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     3181 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     3241 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     3301 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     3361 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     3421 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     3481 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     3541 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     3601 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     3661 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     3721 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     3781 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     3841 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     3901 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     3961 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     4021 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     4081 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     4141 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     4201 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     4261 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     4321 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     4381 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     4441 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     4501 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     4561 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     4621 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     4681 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     4741 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     4801 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     4861 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     4921 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     4981 ctatggaaaa acgccttctt cttgaactcg ggctcggtgc cagtatacct caaatggttg
     5041 tcgtacctct catggttccg ttacgccaac gagggtctgc tgattaacca atgggcggac
     5101 gtggagccgg gcgaaattag ctgcacatcg tcgaacacca cgtgccccag ttcgggcaag
     5161 gtcatcctgg agacgcttaa cttctccgcc gccgatctgc cgctggacta cgtgggtctg
     5221 gcccatgatg aaataacata aggtggtccc gtcgatagcc gaagcttacc gaagtataca
     5281 cttaaattca gtgcacgttt gcttgttgag aggaaaggtt gtgtgcggac gaattttttt
     5341 ttgaaaacat taacccttac gtggaataaa aaaaaatgaa atattgcaaa ttttgctgca
     5401 aagctgtgac tggagtaaaa ttaattcacg tgccgaagtg tgctattaag agaaaattgt
     5461 gggagcagag ccttgggtgc agccttggtg aaaactccca aatttgtgat acccacttta
     5521 atgattcgca gtggaaggct gcacctgcaa aaggtcagac atttaaaagg aggcgactca
     5581 acgcagatgc cgtacctagt aaagtgatag agcctgaacc agaaaagata aaagaaggct
     5641 ataccagtgg gagtacacaa acagagtaag tttgaatagt aaaaaaaatc atttatgtaa
     5701 acaataacgt gactgtgcgt taggtcctgt tcattgttta atgaaaataa gagcttgagg
     5761 gaaaaaattc gtactttgga gtacgaaatg cgtcgtttag agcagcagcc gaattaattc
     5821 tagttccagt gaaatccaag cattttctaa attaaatgta ttcttattat tatagttgtt
     5881 atttttgata tatataaaca acactattat gcccaccatt tttttgagat gcatctacac
     5941 aaggaacaaa cactggatgt cactttcagt tcaaattgta acgctaatca ctccgaacag
     6001 gtcacaaaaa attaccttaa aaagtcataa tattaaatta gaataaatat agctgtgagg
     6061 gaaatatata caaatatatt ggagcaaata aattgtacat acaaatattt attactaatt
     6121 tctattgaga cgaaatgaac cactcggaac catttgagcg aaccgaatcg cgcggaacta
     6181 acgacagtcg ctccaaggtc gtcgaacaaa aggtgaatgt gttgcggaga gcgggtggga
     6241 gacagcgaaa gagcaactac gaaacgtggt gtggtggagg tgaattatga agagggcgcg
     6301 cgatttgaaa agtatgtata taaaaaatat atcccggtgt tttatgtagc gataaacgag
     6361 tttttgatgt aaggtatgca ggtgtgtaag tcttttggtt agaagacaaa tccaaagtct
     6421 acttgtgggg atgttcgaag gggaaatact tgtattctat aggtcatatc ttgtttttat
     6481 tggcacaaat ataattacat tagctttttg agggggcaat aaacagtaaa cacgatggta
     6541 ataatggtaa aaaaaaaaaa caagcagtta tttcggatat atgtcggcta ctccttgcgt
     6601 cgggcccgaa gtcttagagc cagatatgcg agcacccgga agctcacgat gagaatggcc
     6661 agacccacgt agtccagcgg cagatcggcg gcggagaagt taagcgtctc caggatgacc
     6721 ttgcccgaac tggggcacgt ggtgttcgac gatgtgcagc taatttcgcc cggctccacg
     6781 tccgcccatt ggttaatcag cagaccctcg ttggcgtaac ggaaccatga gaggtacgac
     6841 aaccatttga ggtatactgg caccgagccc gagttcaaga agaagccgcc aaagagcagg
     6901 aatggtatga taaccggcgg acccacagac agcgccatcg aggtcgagga gctggcgcag
     6961 gatattagat atccgaagga cgttgacaca ttggccacca gagtgaccag cgccaggcag
     7021 ttgaagaagt gcagcactcc ggcccgcagt ccgatcatcg gataggcaat cgccgtgaag
     7081 accagtggca ctgtgagaaa aagcggcaat tcggcaatcg ttttgcccag aaagtatgtg
     7141 tcacagcgat aaagtcgact tcgggcctcc ctcataaaaa ctggcagctc tgaggtgaac
     7201 acctaaatcg aatcgattca ttagaaagtt agtaaattat tgaaatgcaa atgtattcta
     7261 aacatgactt acatttatcg tggcaaagac gttttgaaag gtcatgttgg tcaggaagag
     7321 gaagatggct ccgttgatat tcatcacacc cacttgcgtg agttgttggc ccaaaaagat
     7381 gaggccaatc aagatggcaa ccatctgcaa attaaaatgt tactcgcatc tcattaatat
     7441 tcgcgagtta aatgaaattt atttatcttc tgcaaaacta taaactatac atctcattga
     7501 aaaaaactaa gaagggtgtg gaatcaggca attctatcta aaatctagcg aatttgtttc
     7561 caagaattgt aagcgttata tcatttgttt ccactggaac cactcaccgt tgtctgaata
     7621 agtcgcactt ttacgaggag tggttccttg agcaccgaca gccaggatcg ccacaggacc
     7681 gcccggaact gcatgaacca ggtggccttg taggtgtacc cattctccgg ctgctccagt
     7741 ggcttctcca gatttttggt ggccaacaac tgctccatat cccgggctac tttgctaatg
     7801 gcaaaattgt cgcatatctt ggcgatccga tcacgggact cgatctcccg tccgggcaca
     7861 acggccaaca cctgtacgta aaagtccgcc ggattgtagt tggtaggaca ctgggcaccc
     7921 acgctggata ggagttgaga tgttatgtaa tactagatac ccttaataaa cacatcgaac
     7981 tcactaggaa aagaagtcga cggcttcgct gggagtgccc aagaaagcta ccctgccctc
     8041 ggccatcaga aggatcttgt caaagagctc aaacagctcg gaagacggct gatgaatggt
     8101 caggatgacg gtcttgccct tctgcgacag cttcttcagc acctggacga cgctgtgggc
     8161 ggtaaaggag tccagtccgg aggtgggctc atcgcagatc agaagcggcg gatcggttag
     8221 agcctcggag gcgaatgcca gacgcttcct ttctccgccg gacagacctt tcaccctgcc
     8281 gggcacaccg atgatcgtgt gctgacattt gctgagcgaa agctcctgga tcacctgatc
     8341 cacgcgggcc actcgctgcc gataggtcag atgtcgtggc atccgcacca tggcttggaa
     8401 aatcaggtgt tccctggccg ttagggagcc gataaagagg tcatcctgct ggacataggc
     8461 gcacctggcc tgcatctcct tggcgtccac aggttggcca ttgagcagtc gcatcccgga
     8521 tggcgatact tggatgccct gcggcgatcg aaaggcaagg gcattcagca gggtcgtctt
     8581 tccggcaccg gaactgccca tcacggccaa aagttcgccc ggataggcca cgccgcaaac
     8641 tgagtttcaa attggtaatt ggacccttta ttaagatttc acacagatca gccgactgcg
     8701 aatagaaact caccgttctt gagcaaatgt ttcctgggcg ccggtatgtg tcgctcgttg
     8761 cagaatagtc cgcgtgtccg gttgaccagc tgccgccatc cggagcccgg ctgattgacc
     8821 gccccaaaga tgtccatatt gtgccaggca taggtgaggt tctcggctag ttggccgctc
     8881 cctgaaccgg agtcctccgg cggactgggt ggccggagcg tgccgtagtt tttggcctgc
     8941 ccgaagccct ggttaatgca gctctgcgaa gccgctccgc tgtcaccctg caatgatagg
     9001 ggatctcaaa tatcaactac aagcgttatg ctcatctaac cccgaacaaa aagtaccccg
     9061 aagtatccta cgaagtaggt ttatactttt atttattttt tgtgcatcta ggatcagctt
     9121 aaaatatctg gttgttatat tttttgtaaa aaagaatata gtcgaaaatg aatgccttta
     9181 gatgtcttga tcatgatatg atctcaaaaa ttgtcttata tagcgagaac agctaccaga
     9241 ataatctgtt tcgtgtcact atttgtttgt gcaattgcgg tttgggattt ttgtgggtcg
     9301 cagttctcac gccgcagaca atttgatgtt gcaatcgcag ttcctataga tcaagtgaac
     9361 ttaagatgta tgcacatgta ctactcacat tgttcagatg ctcggcagat gggtgtttgc
     9421 tgcctccgcg aattaatagc tcctgatcct cttggcccat tgccgggatt tttcacactt
     9481 tcccctgctt acccacccaa aaccaatcac caccccaatc actcaaaaaa caaacaaaaa
     9541 taagaagcga gaggagtttt ggcacagcac tttgtgttta attgatggcg taaaccgctt
     9601 ggagcttcgt cacgaaaccg ctgacaaaat gcaactgaag gcggacattg acgctacgta
     9661 acgctacaaa cggtggcgaa agagatagcg gacgcagcgg cgaaagagac ggcgatattt
     9721 ctgtggacag agaaggaggc aaacagcgct gactttgagt ggaatgtcat tttgagtgag
     9781 aggtaatcga aagaacctgg tacatcaaat acccttggat cgaagtaaat ttaaaactga
     9841 tcagataagt tcaatgatat ccagtgcagt aaaaaaaaaa aatgtttttt ttatctactt
     9901 tccgcaaaaa tgggttttat taacttacat acatactag
//
