SW2 vector (V001592)

MSDS

Basic Vector Information

Vector Name:: SW2

Antibiotic Resistance:: Chloramphenicol

Length:: 6451 bp

Type:: Expression vector

Replication origin:: ori

Source/Author:: Yang XW, Jian HH, Wang FP.

SW2 vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

SW2 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_48872        6451 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector SW2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6451)
  AUTHORS   Yang XW, Jian HH, Wang FP.
  TITLE     A novel low temperature-inducible gene expression vector pSW2 based 
            on a filamentous phage of the deep-sea bacterium Shewanella 
            piezotolerans WP3
  JOURNAL   Appl. Environ. Microbiol. (2015) In press
  PUBMED    26048946
REFERENCE   2  (bases 1 to 6451)
  AUTHORS   Yang X-W.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2015) School of Life Sciences and Biotechnology, 
            Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 
            Shanghai 200240, China
REFERENCE   3  (bases 1 to 6451)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6451)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol. (2015) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-MAR-2015) School of Life Sciences and Biotechnology, Shanghai 
            Jiao Tong University, 800 Dongchuan Road, Shanghai, Shanghai 200240,
            China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             622..2247
                     /codon_start=1
                     /product="FpsA"
                     /label=FpsA
                     /note="putative replication protein"
                     /protein_id="AKM49063.1"
                     /translation="MAEGDIKDLAKEKAQQLTKQLATVDDDAERFRLAERSLSFLGLSF
                     DAATVKRKQEAGELFSMVNRAADEYWLRRQLRRRFAQSVEHVARDLALVQKHKQVYCSD
                     FSVNRYRARTEDNRAALENTVAYDEDDASNVFSLAELSDKSVSNVDNRNAEMFIRLKGF
                     EAVSQSLGHSAIFTTVTAPSRFHSVANRVTNKKWLAAGKPTPVQANDYLVEVWTALRKS
                     LDKKKIKIYGMRVVEPHHDGTPHHHQLIFGLPTDLAFVKKELKRLALLDSPNEKGAKEH
                     RFKCEDIDPNYTKKDGSKGSAVGYIAKYISKSTDGKHIEKDLTTDADAAKSAERIVTWA
                     RIENIRQFQFFGGPSVTVWREMRRLKDEIQEGDAVFNNLEGTEHYLLEKVRKSADEGDW
                     AAFCIAMGGVFVKRTDQTVRTHYAVPAVVEKLFESHEYATTRFGDKANARINGILFQGV
                     FKATRFKNWVVMNKEAYIRGQKKVMSGVVDIFDVLEREKEYERMAEQQYQEYENHMFEC
                     EEMQALLFEASAWRGSEATDVMTH"
     CDS             2309..2623
                     /codon_start=1
                     /product="FpsB"
                     /label=FpsB
                     /note="ssDNA binding protein"
                     /protein_id="AKM49064.1"
                     /translation="MKLEGLILDKSDIQTKQKTNFNTGEVNDVGLIKLITTYPTQTVDV
                     HVSVELMKDPKTLQVLNGVVGTRTQLSVEYKEMSFANSDGKHVAINGFHLFELPHVKAS
                     "
     CDS             2761..2946
                     /codon_start=1
                     /product="FpsC"
                     /label=FpsC
                     /note="putative capsid protein"
                     /protein_id="AKM49065.1"
                     /translation="MSRNSEYEARQKSKGLRKVTLWVPCHCENEFRLMAQSCCEDKDLI
                     PFMLRSLSTGQMRKGS"
     CDS             3040..3279
                     /codon_start=1
                     /product="FpsD"
                     /label=FpsD
                     /note="minor capsid protein"
                     /protein_id="AKM49066.1"
                     /translation="MSQCVAFTQEGYLVQSTQSVDDCTGYILVSPTEYNLAIQSIEIDP
                     ADVLQVFGLVFGWVVFLSFISYKVKIAKVLINRV"
     CDS             complement(3486..4142)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(4143..4245)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(4315..4903)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     oriT            5038..5145
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(6101..6451)
                     /codon_start=1
                     /product="FpsR"
                     /label=FpsR
                     /note="putative replication regulator"
                     /protein_id="AKM49068.1"
                     /translation="MTIGENIKLKRKSLGMTRSELIDRSGVSTAQLSRMERGEQNNPNL
                     ETLVAISTALNTSIDEIVFGEQSTSSIYLSKTIDTLPIDKKTFIKEVIKMAVMISSSEN
                     LENGLKEKKKQG"

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