LOCUS       40924_48872        6451 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector SW2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6451)
  AUTHORS   Yang XW, Jian HH, Wang FP.
  TITLE     A novel low temperature-inducible gene expression vector pSW2 based 
            on a filamentous phage of the deep-sea bacterium Shewanella 
            piezotolerans WP3
  JOURNAL   Appl. Environ. Microbiol. (2015) In press
  PUBMED    26048946
REFERENCE   2  (bases 1 to 6451)
  AUTHORS   Yang X-W.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2015) School of Life Sciences and Biotechnology, 
            Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 
            Shanghai 200240, China
REFERENCE   3  (bases 1 to 6451)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6451)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol. (2015) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-MAR-2015) School of Life Sciences and Biotechnology, Shanghai 
            Jiao Tong University, 800 Dongchuan Road, Shanghai, Shanghai 200240,
            China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             622..2247
                     /codon_start=1
                     /product="FpsA"
                     /label=FpsA
                     /note="putative replication protein"
                     /protein_id="AKM49063.1"
                     /translation="MAEGDIKDLAKEKAQQLTKQLATVDDDAERFRLAERSLSFLGLSF
                     DAATVKRKQEAGELFSMVNRAADEYWLRRQLRRRFAQSVEHVARDLALVQKHKQVYCSD
                     FSVNRYRARTEDNRAALENTVAYDEDDASNVFSLAELSDKSVSNVDNRNAEMFIRLKGF
                     EAVSQSLGHSAIFTTVTAPSRFHSVANRVTNKKWLAAGKPTPVQANDYLVEVWTALRKS
                     LDKKKIKIYGMRVVEPHHDGTPHHHQLIFGLPTDLAFVKKELKRLALLDSPNEKGAKEH
                     RFKCEDIDPNYTKKDGSKGSAVGYIAKYISKSTDGKHIEKDLTTDADAAKSAERIVTWA
                     RIENIRQFQFFGGPSVTVWREMRRLKDEIQEGDAVFNNLEGTEHYLLEKVRKSADEGDW
                     AAFCIAMGGVFVKRTDQTVRTHYAVPAVVEKLFESHEYATTRFGDKANARINGILFQGV
                     FKATRFKNWVVMNKEAYIRGQKKVMSGVVDIFDVLEREKEYERMAEQQYQEYENHMFEC
                     EEMQALLFEASAWRGSEATDVMTH"
     CDS             2309..2623
                     /codon_start=1
                     /product="FpsB"
                     /label=FpsB
                     /note="ssDNA binding protein"
                     /protein_id="AKM49064.1"
                     /translation="MKLEGLILDKSDIQTKQKTNFNTGEVNDVGLIKLITTYPTQTVDV
                     HVSVELMKDPKTLQVLNGVVGTRTQLSVEYKEMSFANSDGKHVAINGFHLFELPHVKAS
                     "
     CDS             2761..2946
                     /codon_start=1
                     /product="FpsC"
                     /label=FpsC
                     /note="putative capsid protein"
                     /protein_id="AKM49065.1"
                     /translation="MSRNSEYEARQKSKGLRKVTLWVPCHCENEFRLMAQSCCEDKDLI
                     PFMLRSLSTGQMRKGS"
     CDS             3040..3279
                     /codon_start=1
                     /product="FpsD"
                     /label=FpsD
                     /note="minor capsid protein"
                     /protein_id="AKM49066.1"
                     /translation="MSQCVAFTQEGYLVQSTQSVDDCTGYILVSPTEYNLAIQSIEIDP
                     ADVLQVFGLVFGWVVFLSFISYKVKIAKVLINRV"
     CDS             complement(3486..4142)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(4143..4245)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(4315..4903)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     oriT            5038..5145
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(6101..6451)
                     /codon_start=1
                     /product="FpsR"
                     /label=FpsR
                     /note="putative replication regulator"
                     /protein_id="AKM49068.1"
                     /translation="MTIGENIKLKRKSLGMTRSELIDRSGVSTAQLSRMERGEQNNPNL
                     ETLVAISTALNTSIDEIVFGEQSTSSIYLSKTIDTLPIDKKTFIKEVIKMAVMISSSEN
                     LENGLKEKKKQG"
ORIGIN
        1 ttttgaacct ttgtgggggc atgtatgact tatttaaact attttcttgc atgtttgatc
       61 tttaggttgt acgcttgcgt catattgatt gcatggatta tatatttgat gtctagaaaa
      121 agatattatt tacaggattt accgacacct ttaactagtt ttactggtgt tcggtcttgt
      181 actactgctg aaaagttcga acttgccgtt caggtttgct tgtcttgcgg tcatgaatat
      241 gaatcggact ctgtagtcac ttgccccgtc tgtgcttcta aactcactta ttcagaagat
      301 tcttttttac ctgacacaaa gaaagcaact tttagcatag ttggcgaagc cacggctaac
      361 ccgcttgatt tgggtttggt tccaacactt ttagataaga tctcttctta ctcattgcca
      421 gttagaacag caaaagaaag ggcggttgcc aaaaccaatt ggcaagcccc cttgtttaat
      481 gttttacgca aacaccgcga ttttgcaccg gcaatgcttc actcttttat ttctatctgc
      541 gataagcgtt cttattttga ggctttacag tcggttgagt ctgcgtccgc tcgacttacc
      601 gaactaggcc acaacgctac aatggcagaa ggtgacatca aagatttagc taaggaaaag
      661 gcgcaacaac tcactaagca gcttgcaact gttgatgacg atgctgagcg attcagactt
      721 gctgagcgct cgctatcatt tcttggtttg tcttttgatg ctgctacggt taagcgaaag
      781 caagaagctg gtgagctatt ttcaatggtt aaccgtgctg ctgatgaata ctggttgagg
      841 cgtcaacttc gccgccgctt cgcgcaatct gtagagcatg tcgctcgtga tttggcattg
      901 gttcaaaagc ataaacaggt ttactgctct gacttttcag tcaatcgcta tagagcacga
      961 acagaagaca atagggcggc tttagagaat accgttgctt atgatgaaga tgatgcaagc
     1021 aatgttttct cacttgctga attgtctgat aaatctgttt ctaatgttga taatcgtaat
     1081 gctgaaatgt tcattcgctt aaaagggttt gaagcggttt ctcagtcttt aggccatagc
     1141 gctattttta caacggttac cgcgcctagt cgctttcatt ctgtcgctaa tcgagtgaca
     1201 aataagaaat ggctagcagc ggggaaacct acgccagttc aagcgaatga ctatctagtc
     1261 gaggtttgga cggcattaag aaagtctctc gataaaaaga aaatcaaaat ctacgggatg
     1321 cgtgttgttg agccgcatca tgacggaaca ccgcatcacc accaattaat ttttggccta
     1381 cctactgatt tagcctttgt aaaaaaagag ctaaagcgct tggcattgct tgactcacca
     1441 aatgaaaagg gtgctaaaga gcatcgtttt aagtgtgaag atatcgaccc caactacacc
     1501 aaaaaagacg gctctaaagg ttctgctgtg ggttatatcg ctaagtacat tagcaaaagc
     1561 acagacggta aacacatcga gaaagacctg acaactgatg ctgatgcagc taaatctgct
     1621 gaaaggattg tgacttgggc acgtattgaa aacattagac agtttcaatt ttttggtggc
     1681 cctagcgtta ccgtatggcg tgaaatgcgc cgcttaaagg atgaaatcca agaaggtgat
     1741 gcagtgttca acaatctaga aggtactgag cattatttgc ttgaaaaggt gcgaaagtct
     1801 gctgatgaag gtgattgggc ggcgttctgt attgctatgg gtggcgtgtt tgttaagcgt
     1861 actgaccaaa ctgttagaac tcattatgca gtccctgcgg tcgttgaaaa actctttgag
     1921 tctcacgagt acgcgacaac tcgcttcggt gataaagcta acgcaagaat caacggcatt
     1981 ctctttcaag gcgtgttcaa agcgactcgt tttaaaaact gggtcgtgat gaataaagag
     2041 gcttacatac gtggccagaa gaaagtcatg tcgggcgttg ttgatatttt cgatgtgctt
     2101 gagcgtgaga aagagtacga gcgcatggcc gaacagcaat accaagaata tgaaaatcac
     2161 atgttcgaat gtgaagagat gcaagctttg ctttttgaag ctagtgcatg gcgtgggagc
     2221 gaagcgaccg acgtcatgac gcactagcct tggactcgtg tcaataagtg tcgtaattgg
     2281 tttttttaaa taaataggaa tacataaaat gaaacttgaa ggcttaattt tagataaatc
     2341 ggatattcaa acaaaacaga aaactaactt caatacaggt gaagtgaatg acgttggttt
     2401 gattaagtta attacaactt acccgaccca aacggtagat gttcatgttt cagtagagct
     2461 tatgaaagac cctaaaacgc tacaggttct taatggcgtt gtgggcactc gtactcagtt
     2521 atcggttgag tataaagaaa tgagctttgc taatagcgat ggcaagcacg ttgctattaa
     2581 tggctttcac ttgtttgaat tgccacacgt taaggcttct taactatgac tgaatttaag
     2641 cgtgttatta cccctattga gtttggcgtt ttgtcgtttt caatacaacg cgcttacatt
     2701 cattggcttt taaatcgggg ctagggcgca ctgtcactgg tgacgctata aagggatctt
     2761 atgagtcgca actctgaata tgaagcaaga cagaaatcta agggtttaag aaaggtaact
     2821 ctttgggttc cttgtcattg tgaaaatgaa tttaggttga tggctcaatc ctgttgtgaa
     2881 gataaagact tgatcccttt tatgcttcgt agtctctcaa caggccaaat gaggaaaggc
     2941 tcataacatg aatacattga tgattttttt aggtggtttc ggctgtggtg gtttagctac
     3001 gtttgtactg cttcattacg cagttaggaa caaataacca tgtctcaatg tgttgctttt
     3061 actcaagaag gttatttagt gcaatccacg caatctgttg atgattgtac gggttacata
     3121 ctggtttcac cgactgaata caatttagct attcagtcga ttgaaataga ccccgccgat
     3181 gttttgcagg tctttgggct tgtctttggt tgggtcgttt tccttagctt tatttcatac
     3241 aaagtgaaaa tagctaaggt gttaatcaat cgcgtctagt taggcgcatt caaataaatg
     3301 taatagaggg atttaaaaat ggctgatatt ttcgctgcgg tagatttagc aagtgttgca
     3361 actttcattg ctggtacagg tgttctgatt gttggtatta accttgcgct taagcgctta
     3421 ttatcactta ttcaggcgta gcaaccaggc gtttaagggc accaataact gccttaaaaa
     3481 aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg
     3541 acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg
     3601 tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg
     3661 gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata
     3721 ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc
     3781 gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac
     3841 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc
     3901 tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg
     3961 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta
     4021 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa
     4081 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc
     4141 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat
     4201 cttatttcat tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc tcattttcgc
     4261 caaaagttgg cccagggctt cccggtatca acagggacac caggatttat ttattttcca
     4321 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4381 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     4441 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     4501 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     4561 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     4621 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     4681 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     4741 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     4801 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     4861 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caattttccg ctgcataacc
     4921 ctgcttcggg gtcattatag cgattttttc ggtatatcca tcctttttcg cacgatatac
     4981 aggattttgc caaagggttc gtgtagactt tccttggtgt atccaacggc gtcagccggg
     5041 caggataggt gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg tcccttattc
     5101 gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc tggcctcgag gagctcggta
     5161 cccggggatc ctctagagtc gacctgcagg catgcacgcg tcgcgtgatt taaggctggc
     5221 aggttatgac gttcaagtct ttggtgattg cgctgtcttg atcacttatg acgactatga
     5281 agactggtta acgtgtgatt cgcctcaagt atctttagca gccaatttac cagaggtcgc
     5341 agaagattag ggagggttcc cgcttgcggg agggttacct aatcgaataa gacctattga
     5401 aaatggagtt taaaatgaaa agaaagttta ccgtttttgt cttcctgatc gtcttgccag
     5461 tgtttagttt tacagtagga atggtttatt cagatataca atggattacg acattagaag
     5521 agattgaggc tagcgaatga ttaaatgtat tacttttttt gttggtctgg ttaggccgct
     5581 agatgtttta ggactagttt gtattgtgat tctatggttt atcacgccat acgttaatga
     5641 gtctctaggg ctttctttga cattttttta agaatgcgca caatttacta atgttatgtt
     5701 gaatactggc cgtccgctaa actgcgcagc agcggagggg atgcacagca gatatttaca
     5761 cataacatac attgtgcgaa ttcctctcga tttatttgtt cctggtcaaa cgttagaaaa
     5821 atctaatgtt tgaccaggac taacttcatt tggttttgtt acgcaatcaa atctattaaa
     5881 tggttacacc attttaatga ccgagcataa taattaatat gttcgggtat cttaatgttt
     5941 attaaatggt gtaaccatac taacaatttt ggggagtatt gaggggattt atccccttga
     6001 tgtgagcgga gcgatacgcc ccttctttaa aaaagccttc gtcctgctag gcatcttttg
     6061 attttacctt gggtgtactt cggtttaatc gattgcttct ttaaccctgt tttttctttt
     6121 cttttaaacc gttttctaga ttttctgagc tgctgatcat tactgccatt tttataactt
     6181 cttttataaa agttttcttg tcgataggta aggtgtcaat tgttttgctt agataaatag
     6241 aacttgtact ttgctctcca aaaacaattt catctattga agtattcagt gctgtggata
     6301 tagcgacaag tgtttctagg tttggattgt tttgttcgcc cctttccatt ctagatagtt
     6361 gtgccgtgga tacaccagac ctatctataa gctctgaacg tgtcattcca aggcttttac
     6421 gcttgagctt tatattttca ccaattgtca t
//
