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scAAV-CBA-null vector (V001597#)

Basic Vector Information

Basic Vector Information
Vector Name scAAV-CBA-null Antibiotic Resistance Ampicillin
Length 5773 bp Type Insertion vector
Source Rosas LE, Grieves JL, Zaraspe K, La Perle KM, Fu H, McCarty DM.

scAAV-CBA-null vector Vector Map

AAV2 terminal repeat CBA promoter AAV2 terminal repeat pEMBL plasmid

scAAV-CBA-null vector Sequence

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LOCUS       Exported                5773 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Insertion vector scAAV-CBA-null, complete sequence.
ACCESSION   JX445135
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   Rosas LE, Grieves JL, Zaraspe K, La Perle KM, Fu H, McCarty DM.
  TITLE     Patterns of scAAV Vector Insertion Associated With Oncogenic Events 
            in a Mouse Model for Genotoxicity
  JOURNAL   Mol. Ther. (2012) In press
  PUBMED    22990674
REFERENCE   2  (bases 1 to 5773)
  AUTHORS   Rosas LE, Grieves JL, Zaraspe K, La Perle KMD., Fu H, McCarty DM.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2012) Pediatrics/Center for Gene Therapy, Research
            Institute at Nationwide Children's Hospital, 700 Children's Dr, 
            Columbus, OH 43205, USA
REFERENCE   3  (bases 1 to 5773)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5773
                     /organism="Insertion vector scAAV-CBA-null"
                     /mol_type="other DNA"
                     /db_xref="taxon:1234868"
     repeat_region   1..112
                     /label=AAV2 terminal repeat
                     /note="AAV2 terminal repeat"
     regulatory      complement(125..2002)
                     /regulatory_class="promoter"
                     /note="CBA promoter"
     intron          complement(125..1200)
                     /note="chimeric rabbit globin/chicken beta-actin intron"
     intron          182..1200
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     promoter        complement(1201..1478)
                     /label=chicken beta-actin promoter
     enhancer        1480..1859
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     repeat_region   2010..2140
                     /label=AAV2 terminal repeat
                     /note="AAV2 terminal repeat"
     misc_feature    2141..5773
                     /label=pEMBL plasmid
                     /note="pEMBL plasmid"
     rep_origin      2557..3070
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3816..3920
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3921..4781
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4952..5540
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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