LOCUS       40924_48763        5773 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Insertion vector scAAV-CBA-null, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   Rosas LE, Grieves JL, Zaraspe K, La Perle KM, Fu H, McCarty DM.
  TITLE     Patterns of scAAV Vector Insertion Associated With Oncogenic Events 
            in a Mouse Model for Genotoxicity
  JOURNAL   Mol. Ther. (2012) In press
  PUBMED    22990674
REFERENCE   2  (bases 1 to 5773)
  AUTHORS   Rosas LE, Grieves JL, Zaraspe K, La Perle KMD., Fu H, McCarty DM.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2012) Pediatrics/Center for Gene Therapy, Research
            Institute at Nationwide Children's Hospital, 700 Children's Dr, 
            Columbus, OH 43205, USA
REFERENCE   3  (bases 1 to 5773)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5773)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Ther. 
            (2012) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-AUG-2012) Pediatrics/Center for Gene Therapy, Research Institute
            at Nationwide Children's Hospital, 700 Children's Dr, Columbus, OH 
            43205, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5773
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..112
                     /label=AAV2 terminal repeat
                     /note="AAV2 terminal repeat"
     intron          complement(182..1200)
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     promoter        complement(1201..1478)
                     /label=chicken beta-actin promoter
     enhancer        1480..1859
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     repeat_region   2010..2140
                     /label=AAV2 terminal repeat
                     /note="AAV2 terminal repeat"
     rep_origin      2557..3070
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3816..3920
                     /label=AmpR promoter
     CDS             3921..4778
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4952..5540
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cagcagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg
       61 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggggttagct
      121 tgatatcgaa ttctttgcca aaatgatgag acagcacaat aaccagcacg ttgcccagga
      181 gctgtaggaa aaagaagaag gcatgaacat ggttagcaga ggctctagag ccgccggtca
      241 cacgccagaa gccgaacccc gccctgcccc gtcccccccg aaggcagccg tccccccgcg
      301 gacagccccg aggctggaga gggagaaggg gacggcggcg cggcgacgca cgaaggccct
      361 ccccgcccat ttccttcctg ccggcgccgc accgcttcgc cccgcgcccg ctagaggggg
      421 tgcggcggcg cctcccagat ttcggctccg cacagatttg ggacaaagta agtccctgcg
      481 ccctctcgca cgattaccat aaaaggcaat ggctgcggct cgccgcgcct cgacagccgc
      541 cggcgctccg ggggccgccg cgcccctccc ccgagccctc cccggcccga ggcggccccg
      601 ccccgcccgg cacccccacc tgccgccacc ccccgcccgg cacggcgagc cccgcgccac
      661 gccccgtacg gagccccgca cccgaagccg ggccgtgctc agcaactcgg ggaggggggt
      721 gcaggggggg gttacagccc gaccgccgcg cccatacccc ctgctcaccc ccccacgcac
      781 acaccccgca cgcagccttt gttcccctcg cagccccccc cgcaccgcgg ggcaccgccc
      841 ccggccgcgc tcccctcgcg cacactgcgg agcgcacaaa gccccgcgcc gcgcccgcag
      901 cgctcacagc cgccgggcag cgcgggccgc acgcggcgct ccccacgcac acacacacgc
      961 acgcaccccc cgagccgctc ccccccgcac aaagggccct cccggagccc ctcaaggctt
     1021 tcacgcagcc acagaaaaga aacaagccgt cattaaacca agcgctaatt acagcccgga
     1081 ggagaagggc cgtcccgccc gctcacctgt gggagtaacg cggtcagtca gagccggggc
     1141 gggcggcgcg aggcggcggc ggagcggggc acggggcgaa ggcagcgtcg cagcgactcc
     1201 cgcccgccgc gcgcttcgct ttttataggg ccgccgccgc cgccgcctcg ccataaaagg
     1261 aaactttcgg agcgcgccgc tctgattggc tgccgccgca cctctccgcc tcgccccgcc
     1321 ccgcccctcg ccccgccccg ccccgcctgg cgcgcgcccc cccccccccc ccgcccccat
     1381 cgctgcacaa aataattaaa aaataaataa atacaaaatt gggggtgggg agggggggga
     1441 gatggggaga gtgaagcaga acgtggggct cacctcgacc atggtaatag cgatgactaa
     1501 tacgtagatg tactgccaag taggaaagtc ccgtaaggtc atgtactggg cataatgcca
     1561 ggcgggccat ttaccgtcat tgacgtcaat agggggcgga cttggcatat gatacacttg
     1621 atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt
     1681 ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt
     1741 gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg
     1801 gctatgaact aatgaccccg taattgatta ctattaataa ctagtcaata atcaatgcca
     1861 acatggcggt catattggac atgagccaat ataaatgtac atattatgat atagatacaa
     1921 cgtatgcaat ggccaatatc caatattgat ttatgctata taaccaatga ataatatggc
     1981 taatggccaa tattgaagat cagcttccta ggaaccccta gtgatggagt tggccactcc
     2041 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
     2101 ctttgcccgg gcggcctcag tgagcgagcg agcgcgccag ctggcgtaat agcgaagagg
     2161 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg aattccagac
     2221 gattgagcgt caaaatgtag gtatttccat gagcgttttt cctgttgcaa tggctggcgg
     2281 taatattgtt ctggatatta ccagcaaggc cgatagtttg agttcttcta ctcaggcaag
     2341 tgatgttatt actaatcaaa gaagtattgc gacaacggtt aatttgcgtg atggacagac
     2401 tcttttactc ggtggcctca ctgattataa aaacacttct caggattctg gcgtaccgtt
     2461 cctgtctaaa atccctttaa tcggcctcct gtttagctcc cgctctgatt ctaacgagga
     2521 aagcacgtta tacgtgctcg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
     2581 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     2641 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     2701 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     2761 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     2821 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     2881 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     2941 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     3001 cgtttacaat ttaaatattt gcttatacaa tcttcctgtt tttggggctt ttctgattat
     3061 caaccggggt acatatgatt gacatgctag ttttacgatt accgttcatc gattctcttg
     3121 tttgctccag actctcaggc aatgacctga tagcctttgt agagacctct caaaaatagc
     3181 taccctctcc ggcatgaatt tatcagctag aacggttgaa tatcatattg atggtgattt
     3241 gactgtctcc ggcctttctc acccgtttga atctttacct acacattact caggcattgc
     3301 atttaaaata tatgagggtt ctaaaaattt ttatccttgc gttgaaataa aggcttctcc
     3361 cgcaaaagta ttacagggtc ataatgtttt tggtacaacc gatttagctt tatgctctga
     3421 ggctttattg cttaattttg ctaattcttt gccttgcctg tatgatttat tggatgttgg
     3481 aattcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     3541 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     3601 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     3661 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     3721 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     3781 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     3841 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     3901 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     3961 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     4021 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     4081 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     4141 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     4201 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     4261 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     4321 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     4381 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     4441 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     4501 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     4561 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     4621 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     4681 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     4741 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     4801 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     4861 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     4921 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     4981 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5041 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     5101 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     5161 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     5221 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     5281 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     5341 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     5401 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     5461 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     5521 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     5581 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     5641 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     5701 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     5761 cgattcatta atg
//
