RescueMu vector (V001607)

Basic Vector Information

      • Vector Name:
      • RescueMu
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 4739 bp
      • Type:
      • Transposition vector
      • Source/Author:
      • Raizada MN, Nan GL, Walbot V.

RescueMu vector Vector Map

RescueMu4739 bp600120018002400300036004200TIRKS primerderived from rhizobiumderived from pBlueScript vectorTIR

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

RescueMu vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                4739 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Transposition vector RescueMu, complete sequence.
ACCESSION   AY301066
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   Raizada MN, Nan GL, Walbot V.
  TITLE     Somatic and germinal mobility of the RescueMu transposon in 
            transgenic maize
  JOURNAL   Plant Cell 13 (7), 1587-1608 (2001)
  PUBMED    11449053
REFERENCE   2  (bases 1 to 4739)
  AUTHORS   Walbot V.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2003) Biological Sciences, Stanford University, 
            385 Serra Mall, Stanford, CA 94305-5020, USA
REFERENCE   3  (bases 1 to 4739)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4739
                     /organism="Transposition vector RescueMu"
                     /mol_type="other DNA"
                     /db_xref="taxon:234691"
     repeat_region   1..210
                     /label=TIR
                     /note="TIR"
     repeat_region   454..560
     primer_bind     761..777
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    784..1216
                     /label=derived from rhizobium
                     /note="derived from rhizobium"
     primer_bind     complement(1213..1229)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1217..4111
                     /label=derived from pBlueScript vector
                     /note="derived from pBlueScript vector"
     promoter        complement(1262..1280)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1287..1303)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(1444..1899)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        1926..2030
                     /gene="bla"
                     /label=AmpR promoter
     gene            2031..2893
                     /gene="bla"
                     /label=bla
     CDS             2031..2891
                     /codon_start=1
                     /gene="bla"
                     /product="beta lactamase"
                     /label=bla
                     /protein_id="AAP69524.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3062..3650
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3938..3959
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3974..4004
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4012..4028
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4036..4052
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4073..4091
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     mobile_element  4105..743
                     /mobile_element_type="transposon:Mu1"
                     /note="derived from Zea mays"
     repeat_region   4220..4323
     repeat_region   4532..4739
                     /label=TIR
                     /note="TIR"

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