Basic Vector Information
RescueMu vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
RescueMu vector Sequence
LOCUS 40924_48678 4739 bp DNA circular SYN 18-DEC-2018
DEFINITION Transposition vector RescueMu, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4739)
AUTHORS Raizada MN, Nan GL, Walbot V.
TITLE Somatic and germinal mobility of the RescueMu transposon in
transgenic maize
JOURNAL Plant Cell 13 (7), 1587-1608 (2001)
PUBMED 11449053
REFERENCE 2 (bases 1 to 4739)
AUTHORS Walbot V.
TITLE Direct Submission
JOURNAL Submitted (16-MAY-2003) Biological Sciences, Stanford University,
385 Serra Mall, Stanford, CA 94305-5020, USA
REFERENCE 3 (bases 1 to 4739)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 4739)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Plant
Cell"; date: "2001"; volume: "13"; issue: "7"; pages: "1587-1608"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(16-MAY-2003) Biological Sciences, Stanford University, 385 Serra
Mall, Stanford, CA 94305-5020, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4739
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..210
/label=TIR
/note="TIR"
repeat_region 454..560
primer_bind 761..777
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1213..1229)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(1262..1280)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(1287..1303)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin complement(1444..1899)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1926..2030
/label=AmpR promoter
CDS 2031..2888
/label=AmpR
/note="beta-lactamase"
rep_origin 3062..3650
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 3938..3959
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 3974..4004
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4012..4028
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 4036..4052
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 4073..4091
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
repeat_region 4220..4323
repeat_region 4532..4739
/label=TIR
/note="TIR"
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