LOCUS       40924_48678        4739 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Transposition vector RescueMu, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   Raizada MN, Nan GL, Walbot V.
  TITLE     Somatic and germinal mobility of the RescueMu transposon in 
            transgenic maize
  JOURNAL   Plant Cell 13 (7), 1587-1608 (2001)
  PUBMED    11449053
REFERENCE   2  (bases 1 to 4739)
  AUTHORS   Walbot V.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2003) Biological Sciences, Stanford University, 
            385 Serra Mall, Stanford, CA 94305-5020, USA
REFERENCE   3  (bases 1 to 4739)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4739)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Cell"; date: "2001"; volume: "13"; issue: "7"; pages: "1587-1608"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-MAY-2003) Biological Sciences, Stanford University, 385 Serra 
            Mall, Stanford, CA 94305-5020, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4739
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..210
                     /label=TIR
                     /note="TIR"
     repeat_region   454..560
     primer_bind     761..777
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1213..1229)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(1262..1280)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1287..1303)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(1444..1899)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1926..2030
                     /label=AmpR promoter
     CDS             2031..2888
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3062..3650
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3938..3959
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3974..4004
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4012..4028
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4036..4052
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4073..4091
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   4220..4323
     repeat_region   4532..4739
                     /label=TIR
                     /note="TIR"
ORIGIN
        1 gagataattg ccattatgga cgaagaggga aggggattcg acgaaatgga ggcgttggcg
       61 ttggcttctc tgttttggag acgcacgcga cagccaaact ccaaaacgga tacgagacag
      121 ctcttggggc tgcgtaaaca ggtattagtt ttctgtcccc gtttaccgtt cccgtgcgca
      181 gacgccgtca cgcgtactct tcttgtctcc gtcgccgcgc tctacggcag cacggcggtg
      241 ctggcgtacg tgctgtactc cgcgccggca gaggccgcgc gcgcgtcgcc cgcggacgac
      301 gccacgccgc tccacctcgc cgcggccgcg cacatcctcc tcgccgcgag cgcgtccgcg
      361 gacgcgcgcg ccttctcggg cctccacgcc ggggacctcc tcctcccgcg cgccaacgag
      421 gccgccgccg ccgcggaccg ggcgctccgc gtgctcctca agttccccgc ggtgtcactg
      481 tcgtcctcgc ccaagaagtc cgcctcgccg ccgccggccc cggaggcgag gaaggagtac
      541 ccgcccgacc tgacgctgcc ggacctcaag agcgggctgt tcagcaccga cgagcaacgg
      601 acgcaccttc cacctgtcct agcactaatt actcctaaat tcatcataca ccaaagtttc
      661 aattagtaaa aggttttggt attattttct ttacaagact aaaagcatcc actcgtattt
      721 gccatggaaa tattgccaaa atggttaccg ggccccccct cgaggtcgac ggtatcgata
      781 agcttgatat cgaattcctg cagcggccgc ggatctcgcc atcatcctca tcgacagccg
      841 gcagggcatt ctccagcaga cccggcgcca ctcctatata gcctcactcc tcggcatccg
      901 ccatgtcgtg ctggccgtca acaagatcga tctcgtcgat tttaaacaac aggtgtacga
      961 ggaaatcgtc gccgactaca tggctttcgc caaagagctc ggtttcgcca gcatacggcc
     1021 aatcccgatc tcggcgcgag acggcgacaa cgtcatctcg gcttccgcca ataccccctg
     1081 gtacagaggg gcggcgctgc tcgaatatct ggaaacggtc gaactcgatc cgacggacca
     1141 ggcaaagcct ttccgcttcc cggttcagat ggtcatgcgg ccgaacgcgg attttcgcgg
     1201 ctatgccggg cagatccact agttctagag cggccgccac cgcggtggag ctccaattcg
     1261 ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa
     1321 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     1381 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     1441 tggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     1501 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     1561 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     1621 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     1681 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     1741 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     1801 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     1861 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa
     1921 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     1981 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     2041 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     2101 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     2161 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     2221 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     2281 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     2341 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     2401 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     2461 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     2521 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     2581 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     2641 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     2701 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     2761 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     2821 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     2881 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     2941 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     3001 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     3061 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     3121 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3181 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     3241 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3301 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3361 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3421 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     3481 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3541 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3601 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3661 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     3721 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     3781 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     3841 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     3901 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     3961 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     4021 gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc gaaattaacc
     4081 ctcactaaag ggaacaaaag ctgggttacc gaaggaacca atgaaaaatg gcggtatccg
     4141 tccacctgta gcttgtccaa ggctcttcca gcagagtagt gccgccatga cgattgacag
     4201 agacacgaga cgaaacaagc tgaaggtccc cgcggcgtca ctgtcgtcct cgccgaagaa
     4261 gtccgcctcg ccgccgccgg ccccggaggc gaggaaggag tacccgcccg acctgacgct
     4321 gcccaccgtg tcgccgccgc cgcccaacgg cctcggcgac atgctcagcc cagcggcctg
     4381 gccctcctcc cccgcgagca ggctcaacaa ggccgcgctc ggcggcggcc gggacctgga
     4441 cctggaccag taccagcaca tgttgttcga caaggtgtcg tcgtcgaggg ccagtaggag
     4501 acaagaagag tacgcgtgac tgagatgcga cggagaaaaa gggtacgcgt gacggcgtct
     4561 gtacacggga acggtaaatg aggacaggaa actaataact gtttacgcag ccccaagtgc
     4621 tgtcttgtgt ccgttttaga gtttggctgt cgcgtacgtc tctaaaacag agaagccaac
     4681 gccaacgcct ctatttcgtc gaatccgctt ctctcttcgt ccataatggc aattatctc
//
