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pShuttle-CMV-lacZ vector (V001805)

Basic Vector Information

Vector Name:
pShuttle-CMV-lacZ
Antibiotic Resistance:
Kanamycin
Length:
10660 bp
Type:
Adenoviral vectors
Replication origin:
ori
Copy Number:
Low copy number
Promoter:
CMV
Cloning Method:
Enzyme digestion and ligation
5' Primer:
pShuttle-CMV-F: GGTCTATATAAGCAGAGCTG
3' Primer:
pShuttle-CMV-R: GTGGTATGGCTGATTATGATCAG

pShuttle-CMV-lacZ vector Vector Map

pShuttle-CMV-lacZ10660 bp5001000150020002500300035004000450050005500600065007000750080008500900095001000010500ITRAd5 PsiCMV enhancerCMV promoterlacZSV40 poly(A) signalIXIVa2ITRoriNeoR/KanRM13 ori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pShuttle-CMV-lacZ vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_40137       10660 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10660)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 10660)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10660
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..103
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     misc_signal     191..340
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        398..701
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        702..905
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             970..4014
                     /label=lacZ
                     /note="beta-galactosidase"
     polyA_signal    4323..4444
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             4527..4946
                     /gene="IX"
                     /label=IX
                     /note="Hexon-interlacing protein from Human adenovirus C 
                     serotype 5. Accession#: P03281"
     CDS             complement(5012..6346)
                     /gene="IVa2"
                     /label=IVa2
                     /note="Packaging protein 1 from Human adenovirus C serotype
                     5. Accession#: P03271"
     repeat_region   7620..7722
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     rep_origin      complement(7982..8570)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             9402..10193
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(10244..10623)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"

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