LOCUS       40924_40137       10660 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10660)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 10660)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10660
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..103
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     misc_signal     191..340
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        398..701
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        702..905
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             970..4014
                     /label=lacZ
                     /note="beta-galactosidase"
     polyA_signal    4323..4444
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             4527..4946
                     /gene="IX"
                     /label=IX
                     /note="Hexon-interlacing protein from Human adenovirus C 
                     serotype 5. Accession#: P03281"
     CDS             complement(5012..6346)
                     /gene="IVa2"
                     /label=IVa2
                     /note="Packaging protein 1 from Human adenovirus C serotype
                     5. Accession#: P03271"
     repeat_region   7620..7722
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     rep_origin      complement(7982..8570)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             9402..10193
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(10244..10623)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt
       61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt
      121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg
      181 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag
      241 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga
      301 agtgaaatct gaataatttt gtgttactca tagcgcgtaa nnnntaatag taatcaatta
      361 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      421 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      481 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      541 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      601 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      661 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      721 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      781 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      841 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      901 gagctggttt agtgaaccgt cagatccgct agagatctgg taccgtcgac gcggccgctc
      961 gagaccatgg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     1021 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     1081 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gctttgcctg gtttccggca
     1141 ccagaagcgg tgccggaaag ctggctggag tgcgatcttc ctgaggccga tactgtcgtc
     1201 gtcccctcaa actggcagat gcacggttac gatgcgccca tctacaccaa cgtgacctat
     1261 cccattacgg tcaatccgcc gtttgttccc acggagaatc cgacgggttg ttactcgctc
     1321 acatttaatg ttgatgaaag ctggctacag gaaggccaga cgcgaattat ttttgatggc
     1381 gttaactcgg cgtttcatct gtggtgcaac gggcgctggg tcggttacgg ccaggacagt
     1441 cgtttgccgt ctgaatttga cctgagcgca tttttacgcg ccggagaaaa ccgcctcgcg
     1501 gtgatggtgc tgcgttggag tgacggcagt tatctggaag atcaggatat gtggcggatg
     1561 agcggcattt tccgtgacgt ctcgttgctg cataaaccga ctacacaaat cagcgatttc
     1621 catgttgcca ctcgctttaa tgatgatttc agccgcgctg tactggaggc tgaagttcag
     1681 atgtgcggcg agttgcgtga ctacctacgg gtaacagttt ctttatggca gggtgaaacg
     1741 caggtcgcca gcggcaccgc gcctttcggc ggtgaaatta tcgatgagcg tggtggttat
     1801 gccgatcgcg tcacactacg tctgaacgtc gaaaacccga aactgtggag cgccgaaatc
     1861 ccgaatctct atcgtgcggt ggttgaactg cacaccgccg acggcacgct gattgaagca
     1921 gaagcctgcg atgtcggttt ccgcgaggtg cggattgaaa atggtctgct gctgctgaac
     1981 ggcaagccgt tgctgattcg aggcgttaac cgtcacgagc atcatcctct gcatggtcag
     2041 gtcatggatg agcagacgat ggtgcaggat atcctgctga tgaagcagaa caactttaac
     2101 gccgtgcgct gttcgcatta tccgaaccat ccgctgtggt acacgctgtg cgaccgctac
     2161 ggcctgtatg tggtggatga agccaatatt gaaacccacg gcatggtgcc aatgaatcgt
     2221 ctgaccgatg atccgcgctg gctaccggcg atgagcgaac gcgtaacgcg aatggtgcag
     2281 cgcgatcgta atcacccgag tgtgatcatc tggtcgctgg ggaatgaatc aggccacggc
     2341 gctaatcacg acgcgctgta tcgctggatc aaatctgtcg atccttcccg cccggtgcag
     2401 tatgaaggcg gcggagccga caccacggcc accgatatta tttgcccgat gtacgcgcgc
     2461 gtggatgaag accagccctt cccggctgtg ccgaaatggt ccatcaaaaa atggctttcg
     2521 ctacctggag agacgcgccc gctgatcctt tgcgaatacg cccacgcgat gggtaacagt
     2581 cttggcggtt tcgctaaata ctggcaggcg tttcgtcagt atccccgttt acagggcggc
     2641 ttcgtctggg actgggtgga tcagtcgctg attaaatatg atgaaaacgg caacccgtgg
     2701 tcggcttacg gcggtgattt tggcgatacg ccgaacgatc gccagttctg tatgaacggt
     2761 ctggtctttg ccgaccgcac gccgcatcca gcgctgacgg aagcaaaaca ccagcagcag
     2821 tttttccagt tccgtttatc cgggcaaacc atcgaagtga ccagcgaata cctgttccgt
     2881 catagcgata acgagctcct gcactggatg gtggcgctgg atggtaagcc gctggcaagc
     2941 ggtgaagtgc ctctggatgt cgctccacaa ggtaaacagt tgattgaact gcctgaacta
     3001 ccgcagccgg agagcgccgg gcaactctgg ctcacagtac gcgtagtgca accgaacgcg
     3061 accgcatggt cagaagccgg gcacatcagc gcctggcagc agtggcgtct ggcggaaaac
     3121 ctcagtgtga cgctccccgc cgcgtcccac gccatcccgc atctgaccac cagcgaaatg
     3181 gatttttgca tcgagctggg taataagcgt tggcaattta accgccagtc aggctttctt
     3241 tcacagatgt ggattggcga taaaaaacaa ctgctgacgc cgctgcgcga tcagttcacc
     3301 cgtgcaccgc tggataacga cattggcgta agtgaagcga cccgcattga ccctaacgcc
     3361 tgggtcgaac gctggaaggc ggcgggccat taccaggccg aagcagcgtt gttgcagtgc
     3421 acggcagata cacttgctga tgcggtgctg attacgaccg ctcacgcgtg gcagcatcag
     3481 gggaaaacct tatttatcag ccggaaaacc taccggattg atggtagtgg tcaaatggcg
     3541 attaccgttg atgttgaagt ggcgagcgat acaccgcatc cggcgcggat tggcctgaac
     3601 tgccagctgg cgcaggtagc agagcgggta aactggctcg gattagggcc gcaagaaaac
     3661 tatcccgacc gccttactgc cgcctgtttt gaccgctggg atctgccatt gtcagacatg
     3721 tataccccgt acgtcttccc gagcgaaaac ggtctgcgct gcgggacgcg cgaattgaat
     3781 tatggcccac accagtggcg cggcgacttc cagttcaaca tcagccgcta cagtcaacag
     3841 caactgatgg aaaccagcca tcgccatctg ctgcacgcgg aagaaggcac atggctgaat
     3901 atcgacggtt tccatatggg gattggtggc gacgactcct ggagcccgtc agtatcggcg
     3961 gaattccagc tgagcgccgg tcgctaccat taccagttgg tctggtgtca aaaataagga
     4021 tccggttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
     4081 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
     4141 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc tagataagat atccgatcca
     4201 ccggatctag ataactgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     4261 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     4321 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     4381 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     4441 cttaacgcnn nntaagggtg ggaaagaata tataaggtgg gggtcttatg tagttttgta
     4501 tctgttttgc agcagccgcc gccgccatga gcaccaactc gtttgatgga agcattgtga
     4561 gctcatattt gacaacgcgc atgcccccat gggccggggt gcgtcagaat gtgatgggct
     4621 ccagcattga tggtcgcccc gtcctgcccg caaactctac taccttgacc tacgagaccg
     4681 tgtctggaac gccgttggag actgcagcct ccgccgccgc ttcagccgct gcagccaccg
     4741 cccgcgggat tgtgactgac tttgctttcc tgagcccgct tgcaagcagt gcagcttccc
     4801 gttcatccgc ccgcgatgac aagttgacgg ctcttttggc acaattggat tctttgaccc
     4861 gggaacttaa tgtcgtttct cagcagctgt tggatctgcg ccagcaggtt tctgccctga
     4921 aggcttcctc ccctcccaat gcggtttaaa acataaataa aaaaccagac tctgtttgga
     4981 tttggatcaa gcaagtgtct tgctgtcttt atttaggggt tttgcgcgcg cggtaggccc
     5041 gggaccagcg gtctcggtcg ttgagggtcc tgtgtatttt ttccaggacg tggtaaaggt
     5101 gactctggat gttcagatac atgggcataa gcccgtctct ggggtggagg tagcaccact
     5161 gcagagcttc atgctgcggg gtggtgttgt agatgatcca gtcgtagcag gagcgctggg
     5221 cgtggtgcct aaaaatgtct ttcagtagca agctgattgc caggggcagg cccttggtgt
     5281 aagtgtttac aaagcggtta agctgggatg ggtgcatacg tggggatatg agatgcatct
     5341 tggactgtat ttttaggttg gctatgttcc cagccatatc cctccgggga ttcatgttgt
     5401 gcagaaccac cagcacagtg tatccggtgc acttgggaaa tttgtcatgt agcttagaag
     5461 gaaatgcgtg gaagaacttg gagacgccct tgtgacctcc aagattttcc atgcattcgt
     5521 ccataatgat ggcaatgggc ccacgggcgg cggcctgggc gaagatattt ctgggatcac
     5581 taacgtcata gttgtgttcc aggatgagat cgtcataggc catttttaca aagcgcgggc
     5641 ggagggtgcc agactgcggt ataatggttc catccggccc aggggcgtag ttaccctcac
     5701 agatttgcat ttcccacgct ttgagttcag atggggggat catgtctacc tgcggggcga
     5761 tgaagaaaac ggtttccggg gtaggggaga tcagctggga agaaagcagg ttcctgagca
     5821 gctgcgactt accgcagccg gtgggcccgt aaatcacacc tattaccggg tgcaactggt
     5881 agttaagaga gctgcagctg ccgtcatccc tgagcagggg ggccacttcg ttaagcatgt
     5941 ccctgactcg catgttttcc ctgaccaaat ccgccagaag gcgctcgccg cccagcgata
     6001 gcagttcttg caaggaagca aagtttttca acggtttgag accgtccgcc gtaggcatgc
     6061 ttttgagcgt ttgaccaagc agttccaggc ggtcccacag ctcggtcacc tgctctacgg
     6121 catctcgatc cagcatatct cctcgtttcg cgggttgggg cggctttcgc tgtacggcag
     6181 tagtcggtgc tcgtccagac gggccagggt catgtctttc cacgggcgca gggtcctcgt
     6241 cagcgtagtc tgggtcacgg tgaaggggtg cgctccgggc tgcgcgctgg ccagggtgcg
     6301 cttgaggctg gtcctgctgg tgctgaagcg ctgccggtct tcgccctgcg cgtcggccag
     6361 gtagcatttg accatggtgt catagtccag cccctccgcg gcgtggccct tggcgcgcag
     6421 cttgcccttg gaggaggcgc cgcacgaggg gcagtgcaga cttttgaggg cgtagagctt
     6481 gggcgcgaga aataccgatt ccggggagta ggcatccgcg ccgcaggccc cgcagacggt
     6541 ctcgcattcc acgagccagg tgagctctgg ccgttcgggg tcaaaaacca ggtttccccc
     6601 atgctttttg atgcgtttct tacctctggt ttccatgagc cggtgtccac gctcggtgac
     6661 gaaaaggctg tccgtgtccc cgtatacaga cttgagaggg agtttaaacg aattcaatag
     6721 cttgttgcat gggcggcgat ataaaatgca aggtgctgct caaaaaatca ggcaaagcct
     6781 cgcgcaaaaa agaaagcaca tcgtagtcat gctcatgcag ataaaggcag gtaagctccg
     6841 gaaccaccac agaaaaagac accatttttc tctcaaacat gtctgcgggt ttctgcataa
     6901 acacaaaata aaataacaaa aaaacattta aacattagaa gcctgtctta caacaggaaa
     6961 aacaaccctt ataagcataa gacggactac ggccatgccg gcgtgaccgt aaaaaaactg
     7021 gtcaccgtga ttaaaaagca ccaccgacag ctcctcggtc atgtccggag tcataatgta
     7081 agactcggta aacacatcag gttgattcat cggtcagtgc taaaaagcga ccgaaatagc
     7141 ccgggggaat acatacccgc aggcgtagag acaacattac agcccccata ggaggtataa
     7201 caaaattaat aggagagaaa aacacataaa cacctgaaaa accctcctgc ctaggcaaaa
     7261 tagcaccctc ccgctccaga acaacataca gcgcttcaca gcggcagcct aacagtcagc
     7321 cttaccagta aaaaagaaaa cctattaaaa aaacaccact cgacacggca ccagctcaat
     7381 cagtcacagt gtaaaaaagg gccaagtgca gagcgagtat atataggact aaaaaatgac
     7441 gtaacggtta aagtccacaa aaaacaccca gaaaaccgca cgcgaaccta cgcccagaaa
     7501 cgaaagccaa aaaacccaca acttcctcaa atcgtcactt ccgttttccc acgttacgta
     7561 acttcccatt ttaagaaaac tacaattccc aacacataca agttactccg ccctaaaacc
     7621 tacgtcaccc gccccgttcc cacgccccgc gccacgtcac aaactccacc ccctcattat
     7681 catattggct tcaatccaaa ataaggtata ttattgatga tgttaattaa catgcatgga
     7741 tccatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
     7801 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     7861 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     7921 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     7981 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8041 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     8101 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8161 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8221 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8281 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     8341 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     8401 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     8461 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     8521 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     8581 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     8641 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     8701 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     8761 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     8821 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     8881 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     8941 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9001 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag
     9061 ccatgagatt atcaaaaagg atcttcacct agatcctttt cacgtagaaa gccagtccgc
     9121 agaaacggtg ctgaccccgg atgaatgtca gctactgggc tatctggaca agggaaaacg
     9181 caagcgcaaa gagaaagcag gtagcttgca gtgggcttac atggcgatag ctagactggg
     9241 cggttttatg gacagcaagc gaaccggaat tgccagctgg ggcgccctct ggtaaggttg
     9301 ggaagccctg caaagtaaac tggatggctt tcttgccgcc aaggatctga tggcgcaggg
     9361 gatcaagctc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     9421 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     9481 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     9541 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc
     9601 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     9661 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     9721 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     9781 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     9841 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     9901 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga
     9961 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
    10021 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
    10081 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
    10141 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgaattt
    10201 tgttaaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaacatc
    10261 ccttataaat caaaagaata gaccgcgata gggttgagtg ttgttccagt ttggaacaag
    10321 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
    10381 gatggcccac tacgtgaacc atcacccaaa tcaagttttt tgcggtcgag gtgccgtaaa
    10441 gctctaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
    10501 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
    10561 gtagcggtca cgctgcgcgt aaccaccaca cccgcgcgct taatgcgccg ctacagggcg
    10621 cgtccattcg ccattcagga tcgaattaat tcttaattaa
//
