pZWHJ002 vector (V001822)

LOCUS       40924_48618        8349 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Promoter probe vector pZWHJ002, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8349)
  AUTHORS   Chao H, Zhou NY.
  TITLE     GenR, an IclR-Type Regulator, Activates and Represses the 
            Transcription of gen Genes Involved in 3-Hydroxybenzoate and 
            Gentisate Catabolism in Corynebacterium glutamicum
  JOURNAL   J. Bacteriol. 195 (7), 1598-1609 (2013)
  PUBMED    23354754
REFERENCE   2  (bases 1 to 8349)
  AUTHORS   Chao H-J., Zhou N-Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JAN-2013) Key Laboratory of Agricultural and 
            Environmental Microbiology, Wuhan Institute of Virology, Chinese 
            Academy of Sciences, No. 44 Xiao Hong Shan, Wu Han, Hubei 430071, 
            P.R. China
REFERENCE   3  (bases 1 to 8349)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8349)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Bacteriol."; date: "2013"; volume: "195"; issue: "7"; pages: 
            "1598-1609"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-JAN-2013) Key Laboratory of Agricultural and Environmental 
            Microbiology, Wuhan Institute of Virology, Chinese Academy of 
            Sciences, No. 44 Xiao Hong Shan, Wu Han, Hubei 430071, P.R. China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..57)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     terminator      265..351
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      443..470
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        489..580
                     /label=AmpR promoter
     rep_origin      complement(967..1555)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      1720..4274
                     /label=ori pBL1
                     /note="ori pBL1"
     CDS             complement(4409..5065)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     regulatory      complement(5131..5225)
                     /label=psiGA1
                     /note="psiGA1"
                     /regulatory_class="promoter"
     CDS             complement(5273..8344)
                     /label=lacZ
                     /note="beta-galactosidase"