LOCUS       40924_48618        8349 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Promoter probe vector pZWHJ002, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8349)
  AUTHORS   Chao H, Zhou NY.
  TITLE     GenR, an IclR-Type Regulator, Activates and Represses the 
            Transcription of gen Genes Involved in 3-Hydroxybenzoate and 
            Gentisate Catabolism in Corynebacterium glutamicum
  JOURNAL   J. Bacteriol. 195 (7), 1598-1609 (2013)
  PUBMED    23354754
REFERENCE   2  (bases 1 to 8349)
  AUTHORS   Chao H-J., Zhou N-Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JAN-2013) Key Laboratory of Agricultural and 
            Environmental Microbiology, Wuhan Institute of Virology, Chinese 
            Academy of Sciences, No. 44 Xiao Hong Shan, Wu Han, Hubei 430071, 
            P.R. China
REFERENCE   3  (bases 1 to 8349)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8349)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Bacteriol."; date: "2013"; volume: "195"; issue: "7"; pages: 
            "1598-1609"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-JAN-2013) Key Laboratory of Agricultural and Environmental 
            Microbiology, Wuhan Institute of Virology, Chinese Academy of 
            Sciences, No. 44 Xiao Hong Shan, Wu Han, Hubei 430071, P.R. China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..57)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     terminator      265..351
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      443..470
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        489..580
                     /label=AmpR promoter
     rep_origin      complement(967..1555)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      1720..4274
                     /label=ori pBL1
                     /note="ori pBL1"
     CDS             complement(4409..5065)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     regulatory      complement(5131..5225)
                     /label=psiGA1
                     /note="psiGA1"
                     /regulatory_class="promoter"
     CDS             complement(5273..8344)
                     /label=lacZ
                     /note="beta-galactosidase"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct cgaattcagc
       61 ttggctgttt tggcggatga gagaagattt tcagcctgat acagattaaa tcagaacgca
      121 gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc ccacctgacc
      181 ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg
      241 cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      301 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga
      361 gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa
      421 actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta
      481 caaactcttt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
      541 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
      601 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
      661 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
      721 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
      781 gagcactttt gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
      841 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
      901 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
      961 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     1021 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     1081 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     1141 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     1201 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     1261 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     1321 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     1381 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     1441 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     1501 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     1561 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     1621 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttggggt gggcgaagaa
     1681 ctccagcatg agatccccgc gctggaggat catccagcca ttcggggtcg ttcactggtt
     1741 cccctttctg atttctggca tagaagaacc cccgtgaact gtgtggttcc gggggttgct
     1801 gatttttgcg agacttctcg cgcaattccc tagcttaggt gaaaacacca tgaaacacta
     1861 gggaaacacc catgaaacac ccattagggc agtagggcgg cttcttcgtc tagggcttgc
     1921 atttgggcgg tgatctggtc tttagcgtgt gaaagtgtgt cgtaggtggc gtgctcaatg
     1981 cactcgaacg tcacgtcatt taccgggtca cggtgggcaa agagaactag tgggttagac
     2041 attgttttcc tcgttgtcgg tggtggtgag cttttctagc cgctcggtaa acgcggcgat
     2101 catgaactct tggaggtttt caccgttctg catgcctgcg cgcttcatgt cctcacgtag
     2161 tgccaaagga acgcgtgcgg tgaccacgac gggcttagcc tttgcctgcg cttctagtgc
     2221 ttcgatggtg gcttgtgcct gcgcttgctg cgcctgtagt gcctgttgag cttcttgtag
     2281 ttgctgttct agctgtgcct tggttgccat gctttaagac tctagtagct ttcctgcgat
     2341 atgtcatgcg catgcgtagc aaacattgtc ctgcaactca ttcattatgt gcagtgctcc
     2401 tgttactagt cgtacatact catatttacc tagtctgcat gcagtgcatg cacatgcagt
     2461 catgtcgtgc taatgtgtaa aacatgtaca tgcagattgc tgggggtgca gggggcggag
     2521 ccaccctgtc catgcggggt gtggggcttg ccccgccggt acagacagtg agcaccgggg
     2581 cacctagtcg cggatacccc ccctaggtat cggacacgta accctcccat gtcgatgcaa
     2641 atctttaaca ttgagtacgg gtaagctggc acgcatagcc aagctaggcg gccaccaaac
     2701 accactaaaa attaatagtc cctagacaag acaaaccccc gtgcgagcta ccaactcata
     2761 tgcacggggg ccacataacc cgaaggggtt tcaattgaca accatagcac tagctaagac
     2821 aacgggcaca acacccgcac aaactcgcac tgcgcaaccc cgcacaacat cgggtctagg
     2881 taacactgag taacactgaa atagaagtga acacctctaa ggaaccgcag gtcaatgagg
     2941 gttctaaggt cactcgcgct agggcgtggc gtaggcaaaa cgtcatgtac aagatcacca
     3001 atagtaaggc tctggcgggg tgccataggt ggcgcaggga cgaagctgtt gcggtgtcct
     3061 ggtcgtctaa cggtgcttcg cagtttgagg gtctgcaaaa ctctcactct cgctgggggt
     3121 cacctctggc tgaattggaa gtcatgggcg aacgccgcat tgagctggct attgctacta
     3181 agaatcactt ggcggcgggt ggcgcgctca tgatgtttgt gggcactgtt cgacacaacc
     3241 gctcacagtc atttgcgcag gttgaagcgg gtattaagac tgcgtactct tcgatggtga
     3301 aaacatctca gtggaagaaa gaacgtgcac ggtacggggt ggagcacacc tatagtgact
     3361 atgaggtcac agactcttgg gcgaacggtt ggcacttgca ccgcaacatg ctgttgttct
     3421 tggatcgtcc actgtctgac gatgaactca aggcgtttga ggattccatg ttttcccgct
     3481 ggtctgctgg tgtggttaag gccggtatgg acgcgccact gcgtgagcac ggggtcaaac
     3541 ttgatcaggt gtctacctgg ggtggagacg ctgcgaaaat ggcaacctac ctcgctaagg
     3601 gcatgtctca ggaactgact ggctccgcta ctaaaaccgc gtctaagggg tcgtacacgc
     3661 cgtttcagat gttggatatg ttggccgatc aaagcgacgc cggcgaggat atggacgctg
     3721 ttttggtggc tcggtggcgt gagtatgagg ttggttctaa aaacctgcgt tcgtcctggt
     3781 cacgtggggc taagcgtgct ttgggcattg attacataga cgctgatgta cgtcgtgaaa
     3841 tggaagaaga actgtacaag ctcgccggtc tggaagcacc ggaacgggtc gaatcaaccc
     3901 gcgttgctgt tgctttggtg aagcccgatg attggaaact gattcagtct gatttcgcgg
     3961 ttaggcagta cgttctcgat tgcgtggata aggctaagga cgtggccgct gcgcaacgtg
     4021 tcgctaatga ggtgctggca agtctgggtg tggattccac cccgtgcatg atcgttatgg
     4081 atgatgtgga cttggacgcg gttctgccta ctcatgggga cgctactaag cgtgatctga
     4141 atgcggcggt gttcgcgggt aatgagcaga ctattcttcg cacccactaa aagcggcata
     4201 aaccccgttc gatattttgt gcgatgaatt tatggtcaat gtcgcggggg caaactatga
     4261 tgggtcttgt tgttggcgtc ccggaaaacg attccgaagc ccaacctttc atagaaggcg
     4321 gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt catttcgaag
     4381 ggcaccaata actgccttaa aaaaattacg ccccgccctg ccactcatcg cagtactgtt
     4441 gtaattcatt aagcattctg ccgacatgga agccatcaca gacggcatga tgaacctgaa
     4501 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
     4561 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
     4621 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
     4681 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
     4741 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
     4801 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacggaac tccggatgag
     4861 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
     4921 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
     4981 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
     5041 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgtcgaag
     5101 ctcggcggat ttgtcctact caagctgatc cgacaaaatc cacacattat cccaggtgtc
     5161 cggatcggtc aaatacgctg ccagctcata gaccgtatcc aaagcatccg gggctgatcc
     5221 ccggcgccct tacgcgaaat acgggcagac atggcctgcc cggttattat tatttttgac
     5281 accagaccaa ctggtaatgg tagcgaccgg cgctcagctg gaattccgcc gatactgacg
     5341 ggctccagga gtcgtcgcca ccaatcccca tatggaaacc gtcgatattc agccatgtgc
     5401 cttcttccgc gtgcagcaga tggcgatggc tggtttccat cagttgctgt tgactgtagc
     5461 ggctgatgtt gaactggaag tcgccgcgcc actggtgtgg gccataattc aattcgcgcg
     5521 tcccgcagcg cagaccgttt tcgctcggga agacgtacgg ggtatacatg tctgacaatg
     5581 gcagatccca gcggtcaaaa caggcggcag taaggcggtc gggatagttt tcttgcggcc
     5641 ctaatccgag ccagtttacc cgctctgcta cctgcgccag ctggcagttc aggccaatcc
     5701 gcgccggatg cggtgtatcg ctcgccactt caacatcaac ggtaatcgcc atttgaccac
     5761 taccatcaat ccggtaggtt ttccggctga taaataaggt tttcccctga tgctgccacg
     5821 cgtgagcggt cgtaatcagc accgcatcag caagtgtatc tgccgtgcac tgcaacaacg
     5881 ctgcttcggc ctggtaatgg cccgccgcct tccagcgttc gacccaggcg ttagggtcaa
     5941 tgcgggtcgc ttcacttacg ccaatgtcgt tatccagcgg tgcacgggtg aactgatcgc
     6001 gcagcggcgt cagcagttgt tttttatcgc caatccacat ctgtgaaaga aagcctgact
     6061 ggcggttaaa ttgccaacgc ttattaccca gctcgatgca aaaatccatt tcgctggtgg
     6121 tcagatgcgg gatggcgtgg gacgcggcgg ggagtgtcac gctgaggttt tcagccagac
     6181 gccactgctg ccaggcgctg atgtgtccgg cttctgacca tgcggtcgcg ttcggttgca
     6241 ctacgcgtac tgtgagccag agttgcccgg cgctctccgg ctgcggtagt tcaggcagtt
     6301 caatcaactg tttaccttgt ggagcgacat ccagaggcac ttcaccgctt gccagcggct
     6361 taccatccag cgccaccatc cagtgcagga gctcgttatc gctatgacgg aacaggtatt
     6421 cgctggtcac ttcgatggtt tgcccggata aacggaactg gaaaaactgc tgctggtgtt
     6481 ttgcttccgt cagcgctgga tgcggcgtgc ggtcggcaaa gaccagaccg ttcatacaga
     6541 actggcgatc gttcggcgta tcgccaaaat caccgccgta agccgaccac gggttgccgt
     6601 tttcatcata tttaatcagc gactgatcca cccagtccca gacgaagccg ccctgtaaac
     6661 ggggatactg acgaaacgcc tgccagtatt tagcgaaacc gccaagactg ttacccatcg
     6721 cgtgggcgta ttcgcaaagg atcagcgggc gcgtctctcc aggtagcgaa agccattttt
     6781 tgatggacca tttcggcaca gccgggaagg gctggtcttc atccacgcgc gcgtacatcg
     6841 ggcaaataat atcggtggcc gtggtgtcgg ctccgccgcc ttcatactgc accgggcggg
     6901 aaggatcgac agatttgatc cagcgataca gcgcgtcgtg attagcgccg tggcctgatt
     6961 cattccccag cgaccagatg atcacactcg ggtgattacg atcgcgctgc accattcgcg
     7021 ttacgcgttc gctcatcgcc ggtagccagc gcggatcatc ggtcagacga ttcattggca
     7081 ccatgccgtg ggtttcaata ttggcttcat ccaccacata caggccgtag cggtcgcaca
     7141 gcgtgtacca cagcggatgg ttcggataat gcgaacagcg cacggcgtta aagttgttct
     7201 gcttcatcag caggatatcc tgcaccatcg tctgctcatc catgacctga ccatgcagag
     7261 gatgatgctc gtgacggtta acgcctcgaa tcagcaacgg cttgccgttc agcagcagca
     7321 gaccattttc aatccgcacc tcgcggaaac cgacatcgca ggcttctgct tcaatcagcg
     7381 tgccgtcggc ggtgtgcagt tcaaccaccg cacgatagag attcgggatt tcggcgctcc
     7441 acagtttcgg gttttcgacg ttcagacgta gtgtgacgcg atcggcataa ccaccacgct
     7501 catcgataat ttcaccgccg aaaggcgcgg tgccgctggc gacctgcgtt tcaccctgcc
     7561 ataaagaaac tgttacccgt aggtagtcac gcaactcgcc gcacatctga acttcagcct
     7621 ccagtacagc gcggctgaaa tcatcattaa agcgagtggc aacatggaaa tcgctgattt
     7681 gtgtagtcgg tttatgcagc aacgagacgt cacggaaaat gccgctcatc cgccacatat
     7741 cctgatcttc cagataactg ccgtcactcc agcgcagcac catcaccgcg aggcggtttt
     7801 ctccggcgcg taaaaatgcg ctcaggtcaa attcagacgg caaacgactg tcctggccgt
     7861 aaccgaccca gcgcccgttg caccacagat gaaacgccga gttaacgcca tcaaaaataa
     7921 ttcgcgtctg gccttcctgt agccagcttt catcaacatt aaatgtgagc gagtaacaac
     7981 ccgtcggatt ctccgtggga acaaacggcg gattgaccgt aatgggatag gtcacgttgg
     8041 tgtagatggg cgcatcgtaa ccgtgcatct gccagtttga ggggacgacg acagtatcgg
     8101 cctcaggaag atcgcactcc agccagcttt ccggcaccgc ttctggtgcc ggaaaccagg
     8161 caaagcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     8221 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     8281 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaatcc gtaatcatgg
     8341 tcatagctg
//
