Basic Vector Information
- Vector Name:
- PZPnat1
- Antibiotic Resistance:
- Kanamycin
- Length:
- 8489 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Gardiner DM, Howlett BJ.
- Promoter:
- trpC
PZPnat1 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
PZPnat1 vector Sequence
LOCUS 40924_48548 8489 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector PZPnat1, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8489)
AUTHORS Gardiner DM, Howlett BJ.
TITLE Direct Submission
JOURNAL Submitted (21-MAY-2004) School of Botany, The University of
Melbourne, Melbourne, VIC 3010, Australia
REFERENCE 2 (bases 1 to 8489)
AUTHORS Gardiner DM, Howlett BJ.
TITLE Direct Submission
JOURNAL Submitted (13-MAR-2014) School of Botany, The University of
Melbourne, Melbourne, VIC 3010, Australia
REFERENCE 3 (bases 1 to 8489)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 8489)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
(21-MAY-2004) School of Botany, The University of Melbourne,
Melbourne, VIC 3010, Australia"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(13-MAR-2014) School of Botany, The University of Melbourne,
Melbourne, VIC 3010, Australia"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT On Mar 13, 2014 this sequence version replaced AY631958.1.
FEATURES Location/Qualifiers
source 1..8489
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature complement(114..138)
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
primer_bind 334..350
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 380..396
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
promoter 441..801
/label=trpC promoter
/note="promoter for Aspergillus nidulans trpC"
CDS 818..1384
/label=NrsR
/note="nourseothricin acetyltransferase"
regulatory 1398..2115
/label=TrpC
/note="TrpC"
/regulatory_class="terminator"
primer_bind complement(2137..2153)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(2161..2177)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(2185..2215)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(2230..2251)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
misc_feature complement(2428..2452)
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
CDS 2745..3557
/label=KanR
/note="aminoglycoside phosphotransferase"
rep_origin 3655..4243
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(4429..4569)
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(4913..5107)
/direction=LEFT
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
CDS complement(5176..6240)
/label=pVS1 RepA
/note="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
CDS complement(6677..7303)
/label=pVS1 StaA
/note="stability protein from the Pseudomonas plasmid pVS1
(Heeb et al., 2000)"
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