PZPnat1 vector (V001828)

LOCUS       40924_48548        8489 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector PZPnat1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8489)
  AUTHORS   Gardiner DM, Howlett BJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2004) School of Botany, The University of 
            Melbourne, Melbourne, VIC 3010, Australia
REFERENCE   2  (bases 1 to 8489)
  AUTHORS   Gardiner DM, Howlett BJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2014) School of Botany, The University of 
            Melbourne, Melbourne, VIC 3010, Australia
REFERENCE   3  (bases 1 to 8489)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8489)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (21-MAY-2004) School of Botany, The University of Melbourne, 
            Melbourne, VIC 3010, Australia"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-MAR-2014) School of Botany, The University of Melbourne, 
            Melbourne, VIC 3010, Australia"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     On Mar 13, 2014 this sequence version replaced AY631958.1.
FEATURES             Location/Qualifiers
     source          1..8489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(114..138)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     334..350
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     380..396
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        441..801
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     CDS             818..1384
                     /label=NrsR
                     /note="nourseothricin acetyltransferase"
     regulatory      1398..2115
                     /label=TrpC
                     /note="TrpC"
                     /regulatory_class="terminator"
     primer_bind     complement(2137..2153)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2161..2177)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2185..2215)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2230..2251)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    complement(2428..2452)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2745..3557
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      3655..4243
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(4429..4569)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4913..5107)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5176..6240)
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6677..7303)
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"