LOCUS       40924_48548        8489 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector PZPnat1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8489)
  AUTHORS   Gardiner DM, Howlett BJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2004) School of Botany, The University of 
            Melbourne, Melbourne, VIC 3010, Australia
REFERENCE   2  (bases 1 to 8489)
  AUTHORS   Gardiner DM, Howlett BJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2014) School of Botany, The University of 
            Melbourne, Melbourne, VIC 3010, Australia
REFERENCE   3  (bases 1 to 8489)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8489)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (21-MAY-2004) School of Botany, The University of Melbourne, 
            Melbourne, VIC 3010, Australia"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-MAR-2014) School of Botany, The University of Melbourne, 
            Melbourne, VIC 3010, Australia"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     On Mar 13, 2014 this sequence version replaced AY631958.1.
FEATURES             Location/Qualifiers
     source          1..8489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(114..138)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     334..350
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     380..396
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        441..801
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     CDS             818..1384
                     /label=NrsR
                     /note="nourseothricin acetyltransferase"
     regulatory      1398..2115
                     /label=TrpC
                     /note="TrpC"
                     /regulatory_class="terminator"
     primer_bind     complement(2137..2153)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2161..2177)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2185..2215)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2230..2251)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    complement(2428..2452)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2745..3557
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      3655..4243
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(4429..4569)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4913..5107)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5176..6240)
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6677..7303)
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
ORIGIN
        1 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
       61 ttttcacgcc cttttaaata tccgttattc taataaacgc tcttttctct taggtttacc
      121 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      181 tccaagctca agctcattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
      241 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
      301 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctag
      361 gcctcaccgc ggcccatggt ctagaactag tggatccgaa ttcgttaaca agcttgtcga
      421 cctcgagggt accgattcga cagaagatga cattgaagga gcactttttg ggcttggctg
      481 gagctagtgg aggtcaacaa tgaatgccta ttttggttta gtcgtccagg cggtgagcac
      541 aaaatttgtg tcgtttgaca agatggttca tttaggcaac tggtcagatc agccccactt
      601 gtagcagtag cggcggcgct cgaagtgtga ctcttattag cagacaggaa cgaggacatt
      661 attatcatct gctgcttggt gcacgataac ttggtgcgtt tgtcaagcaa ggtaagtgaa
      721 cgacccggtc ataccttctt aagttcgccc ttcctccctt tatttcagat tcaatctgac
      781 ttacctattc tacccaagca tcgataagct gatccatatg accactcttg acgacacggc
      841 ttaccggtac cgcaccagtg tcccggggga cgccgaggcc atcgaggcac tggatgggtc
      901 cttcaccacc gacaccgtct tccgcgtcac cgccaccggg gacggcttca ccctgcggga
      961 ggtgccggtg gacccgcccc tgaccaaggt gttccccgac gacgaatcgg acgacgaatc
     1021 ggacgacggg gaggacggcg acccggactc ccggacgttc gtcgcgtacg gggacgacgg
     1081 cgacctggcg ggcttcgtgg tcgtctcgta ctccggctgg aaccgccggc tgaccgtcga
     1141 ggacatcgag gtcgccccgg agcaccgggg gcacggggtc gggcgcgcgt tgatggggct
     1201 cgcgacggag ttcgcccgcg agcggggcgc cgggcacctc tggctggagg tcaccaacgt
     1261 caacgcaccg gcgatccacg cgtaccggcg gatggggttc accctctgcg gcctggacac
     1321 cgccctgtac gacggcaccg cctcggacgg cgagcaggcg ctctacatga gcatgccctg
     1381 cccctgagcg gccgatccac ttaacgttac tgaaatcatc aaacagcttg acgaatctgg
     1441 atataagatc gttggtgtcg atgtcagctc cggagttgag acaaatggtg ttcaggatct
     1501 cgataagata cgttcatttg tccaagcagc aaagagtgcc ttctagtgat ttaatagctc
     1561 catgtcaaca agaataaaac gcgtttcggg tttacctctt ccagatacag ctcatctgca
     1621 atgcattaat gcattggacc tcgcaaccct agtacgccct tcaggctccg gcgaagcaga
     1681 agaatagctt agcagagtct attttcattt tcgggagacg agatcaagca gatcaacggt
     1741 cgtcaagaga cctacgagac tgaggaatcc gctcttggct ccacgcgact atatatttgt
     1801 ctctaattgt actttgacat gctcctcttc tttactctga tagcttgact atgaaaattc
     1861 cgtcaccagc ccctgggttc gcaaagataa ttgcactgtt tcttccttga actctcaagc
     1921 ctacaggaca cacattcatc gtaggtataa acctcgaaaa tcattcctac taagatgggt
     1981 atacaatagt aaccatggtt gcctagtgaa tgctccgtaa cacccaatac gccggccgaa
     2041 acttttttac aactctccta tgagtcgttt acccagaatg cacaggtaca cttgtttaga
     2101 gggctcgtcg aaatcgaatt atcaattcgt aatcatgtca tagctgtttc ctgtgtgaaa
     2161 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     2221 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     2281 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     2341 tttgcgtatt ggctagagca attcggcgtt aattcagtac attaaaaacg tccgcaatgt
     2401 gttattaagt tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag
     2461 ccaacagctc cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc
     2521 agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc
     2581 gatgctattc ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg
     2641 agggtagcat gttgattgta acgatgacag agcgttgctg cctgtgatcc agatcatgaa
     2701 caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac
     2761 gggaaacgtc ttgctctagg ccgcgattaa attccaacat ggatgctgat ttatatgggt
     2821 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga ttgtatggga
     2881 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta
     2941 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc
     3001 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc gggaaaacag
     3061 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag
     3121 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg
     3181 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt
     3241 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt
     3301 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt
     3361 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat
     3421 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac
     3481 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga
     3541 tgctcgatga gtttttctaa gaattaattc atgatcctgc atgaccaaaa tcccttaacg
     3601 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     3661 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     3721 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     3781 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     3841 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     3901 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     3961 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4021 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     4081 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4141 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4201 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     4261 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     4321 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     4381 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     4441 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     4501 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     4561 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     4621 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     4681 tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag
     4741 tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc cagccatttt
     4801 tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga
     4861 ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca
     4921 ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc
     4981 ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg
     5041 gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct
     5101 atccacagga aagagacctt ttcgaccttt ttcccctgct agggcaattt gccctagcat
     5161 ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat
     5221 gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt
     5281 tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact
     5341 gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc
     5401 caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt
     5461 cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat
     5521 ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc
     5581 ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc
     5641 aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc
     5701 atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg
     5761 cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat
     5821 cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac
     5881 gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag
     5941 acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat
     6001 cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc
     6061 ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc
     6121 accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt
     6181 ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc
     6241 gtcacgccga ttcctcgggc ttgggggttc cagtgccatt gcagggccgg cagacaaccc
     6301 agccgcttac gcctggccaa ccgcccgttc ctccacacat ggggcattcc acggcgtcgg
     6361 tgcctggttg ttcttgattt tccatgccgc ctcctttagc cgctaaaatt catctactca
     6421 tttattcatt tgctcattta ctctggtagc tgcgcgatgt attcagatag cagctcggta
     6481 atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac
     6541 tgaaagttga cccgcttcat ggctggcgtg tctgccaggc tggccaacgt tgcagccttg
     6601 ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg tgcttttgct cattttctct
     6661 ttacctcatt aactcaaatg agttttgatt taatttcagc ggccagcgcc tggacctcgc
     6721 gggcagcgtc gccctcgggt tctgattcaa gaacggttgt gccggcggcg gcagtgcctg
     6781 ggtagctcac gcgctgcgtg atacgggact caagaatggg cagctcgtac ccggccagcg
     6841 cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg
     6901 cttgtagcct tccatccgtg acctcaatgc gctgcttaac cagctccacc aggtcggcgg
     6961 tggcccatat gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg gctgccttga
     7021 tcgcggacac agccaagtcc gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc
     7081 ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca acggttagcg
     7141 gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg gaatcgacta
     7201 acagaacatc ggccccggcg agttgcaggg cgcgggctag atgggttgcg atggtcgtct
     7261 tgcctgaccc gcctttctgg ttaagtacag cgataacctt catgcgttcc ccttgcgtat
     7321 ttgtttattt actcatcgca tcatatacgc agcgaccgca tgacgcaagc tgttttactc
     7381 aaatacacat caccttttta gacggcggcg ctcggtttct tcagcggcca agctggccgg
     7441 ccaggccgcc agcttggcat cagacaaacc ggccaggatt tcatgcagcc gcacggttga
     7501 gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc atctccgcct cgatctcttc
     7561 ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg
     7621 cgttcattct cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc acggaaggca
     7681 ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg
     7741 gtcgagcgat gcacgccaag cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg
     7801 atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc
     7861 acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc
     7921 tcgaactcgg caatgccggc gaacacggtc aacaccatgc ggccggccgg cgtggtggtg
     7981 tcggcccacg gctctgccag gctacgcagg cccgcgccgg cctcctggat gcgctcggca
     8041 atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg
     8101 tcgccagggc gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg cctggtgccg
     8161 gtgatcttct cggaaaacag cttggtgcag ccggccgcgt gcagttcggc ccgttggttg
     8221 gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc ggcggcgctc
     8281 ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat gcgactaaaa
     8341 cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt aacttaggac
     8401 ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta
     8461 tagcagcgga ggggttggat caaagtact
//
