pZNR0964 vector (V001829)

LOCUS       V001829                 8009 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001829
VERSION     V001829
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8009)
  AUTHORS   DeLoache WC, Russ ZN, Narcross L, Gonzales AM, Martin VJ, Dueber JE.
  TITLE     An enzyme-coupled biosensor enables (S)-reticuline production in
            yeast from glucose
  JOURNAL   Nat. Chem. Biol. 11 (7), 465-471 (2015)
   PUBMED   25984720
REFERENCE   2  (bases 1 to 8009)
  AUTHORS   DeLoache WC, Russ ZN, Narcross L, Gonzales AM, Martin VJJ., Dueber
            JE.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-APR-2015) Department of Bioengineering, University of
            California, Berkeley, 2151 Berkeley Way Room 542, Berkeley, CA
            94704, USA
REFERENCE   3  (bases 1 to 8009)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8009)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Chem.
            Biol."; date: "2015"; volume: "11"; issue: "7"; pages: "465-471"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-APR-2015) Department of Bioengineering, University of
            California, Berkeley, 2151 Berkeley Way Room 542, Berkeley, CA
            94704, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    12..17
                     /label="BsmBI recognition site"
                     /note="BsmBI recognition site"
     misc_feature    19..22
                     /label="BsmBI recognition site"
                     /note="BsmBI recognition site"
     regulatory      189..888
                     /regulatory_class="promoter"
     CDS             895..2385
                     /gene="CYP76AD1"
                     /label="Cytochrome P450 76AD1"
                     /note="Cytochrome P450 76AD1 from Beta vulgaris.
                     Accession#: I3PFJ5"
     CDS             2392..3084
                     /label="mKate2"
                     /note="monomeric far-red fluorescent protein (Shcherbo et
                     al., 2009)"
     terminator      3094..3281
                     /label="ADH1 terminator"
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     misc_feature    complement(3488..3491)
                     /label="BsmBI recognition site"
                     /note="BsmBI recognition site"
     misc_feature    complement(3493..3498)
                     /label="BsmBI recognition site"
                     /note="BsmBI recognition site"
     misc_feature    3510..4013
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     regulatory      complement(4019..4495)
                     /regulatory_class="terminator"
     CDS             complement(4499..5590)
                     /label="LEU2"
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     promoter        complement(5591..5998)
                     /label="LEU2 promoter"
     rep_origin      complement(6354..6942)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     regulatory      7019..7166
                     /regulatory_class="promoter"
     CDS             7167..7979
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     regulatory      7983..8009
                     /regulatory_class="terminator"