LOCUS       V001829                 8009 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001829
VERSION     V001829
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8009)
  AUTHORS   DeLoache WC, Russ ZN, Narcross L, Gonzales AM, Martin VJ, Dueber JE.
  TITLE     An enzyme-coupled biosensor enables (S)-reticuline production in
            yeast from glucose
  JOURNAL   Nat. Chem. Biol. 11 (7), 465-471 (2015)
   PUBMED   25984720
REFERENCE   2  (bases 1 to 8009)
  AUTHORS   DeLoache WC, Russ ZN, Narcross L, Gonzales AM, Martin VJJ., Dueber
            JE.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-APR-2015) Department of Bioengineering, University of
            California, Berkeley, 2151 Berkeley Way Room 542, Berkeley, CA
            94704, USA
REFERENCE   3  (bases 1 to 8009)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8009)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Chem.
            Biol."; date: "2015"; volume: "11"; issue: "7"; pages: "465-471"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-APR-2015) Department of Bioengineering, University of
            California, Berkeley, 2151 Berkeley Way Room 542, Berkeley, CA
            94704, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    12..17
                     /label="BsmBI recognition site"
                     /note="BsmBI recognition site"
     misc_feature    19..22
                     /label="BsmBI recognition site"
                     /note="BsmBI recognition site"
     regulatory      189..888
                     /regulatory_class="promoter"
     CDS             895..2385
                     /gene="CYP76AD1"
                     /label="Cytochrome P450 76AD1"
                     /note="Cytochrome P450 76AD1 from Beta vulgaris.
                     Accession#: I3PFJ5"
     CDS             2392..3084
                     /label="mKate2"
                     /note="monomeric far-red fluorescent protein (Shcherbo et
                     al., 2009)"
     terminator      3094..3281
                     /label="ADH1 terminator"
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     misc_feature    complement(3488..3491)
                     /label="BsmBI recognition site"
                     /note="BsmBI recognition site"
     misc_feature    complement(3493..3498)
                     /label="BsmBI recognition site"
                     /note="BsmBI recognition site"
     misc_feature    3510..4013
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     regulatory      complement(4019..4495)
                     /regulatory_class="terminator"
     CDS             complement(4499..5590)
                     /label="LEU2"
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     promoter        complement(5591..5998)
                     /label="LEU2 promoter"
     rep_origin      complement(6354..6942)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     regulatory      7019..7166
                     /regulatory_class="promoter"
     CDS             7167..7979
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     regulatory      7983..8009
                     /regulatory_class="terminator"
ORIGIN
        1 gaattcgttc acgtctccgt tctggtagag ccacaaacag ccggtacaag caacgatctc
       61 caggaccatc tgaatcatgc gcggatgaca cgaactcacg acggcgatca cagacattaa
      121 cccacagccg ataattgcag acggtacaga cactgcgaca acgtggcaat tcgtcgcaat
      181 actctagaaa gaggatgtcc aatatttttt ttaaggaata aggatacttc aagactagat
      241 tcccccctgc attcccatca gaaccgtaaa ccttggcgct ttccttggga agtattcaag
      301 aagtgccttg tccggtttct gtggctcaca aaccagcgcg cccgatatgg ctttcttttc
      361 acttatgaat gtaccagtac gggacaatta gaacgctcct gtaacaatct ctttgcaaat
      421 gtggggttac attctaacca tgtcacactg ctgacgaaat tcaaagtaaa aaaaaatggg
      481 accacgtctt gagaacgata gattttcttt attttacatt gaacagtcgt tgtctcagcg
      541 cgctttatgt tttcattcat acttcatatt ataaaataac aaaagaagaa tttcatattc
      601 acgcccaaga aatcaggctg ctttccaaat gcaattgaca cttcattagc catcacacaa
      661 aactctttct tgctggagct tcttttaaaa aagacctcag tacaccaaac acgttacccg
      721 acctcgttat tttacgacaa ctatgataaa attctgaaga aaaaataaaa aaattttcat
      781 acttcttgct tttatttaaa ccattgaatg atttcttttg aacaaaacta cctgtttcac
      841 caaaggaaat agaaagaaaa aatcaattag aagaaaacaa aaaacaaaag atctatggat
      901 catgcaacat tagctatgat attagcaatt tggtttattt catttcattt tattaaatta
      961 ttattctcac aacaaacaac aaaattgtta cctccaggtc ctaaaccttt accaattatt
     1021 ggtaatatct tggaagtagg aaagaagcct catagatcat ttgcaaattt ggctaagatc
     1081 cacggtcctt taatctcttt gaggttagga tcagttacca ccattgttgt atcatctgct
     1141 gatgtcgcca aagagatgtt cttgaagaag gatcaccctt tatcaaacag gacaattcca
     1201 aattcagtaa ctgctggtga ccaccacaaa ttgactatgt cttggttgcc tgtttcacct
     1261 aaatggagaa acttcagaaa gataactgct gttcatttat tgtctccaca aagattagat
     1321 gcatgccaga cctttagaca tgcaaaggtt cagcaattat atgaatacgt tcaagagtgc
     1381 gctcagaagg gtcaggcagt tgacattggt aaagctgcct tcacaacttc tttgaattta
     1441 ttgtctaaat tattcttttc tgttgaattg gcccatcata aatcacatac ctctcaagag
     1501 tttaaggaat taatttggaa tattatggaa gacatcggta aaccaaacta cgccgattac
     1561 ttcccaattt taggttgcgt cgatccatca ggaattagaa gaaggttggc atgttctttt
     1621 gataagttaa ttgcagtatt tcaaggaata atatgtgaga ggttagctcc agattcatca
     1681 actacaacta ctacaactac agatgatgtc ttggacgtat tattgcaatt gtttaagcag
     1741 aatgaattaa ctatgggaga gattaaccac ttgttagtcg atattttcga tgccggtact
     1801 gatactacat catctacctt tgagtgggtt atgactgagt taataagaaa ccctgaaatg
     1861 atggaaaagg cccaagagga aataaaacag gtattgggta aggataagca aatccaagag
     1921 tcagacataa taaatttgcc atacttgcaa gcaatcatta aggaaacttt gaggttgcac
     1981 ccacctacag ttttcttgtt gcctagaaaa gccgacacag acgtagaatt gtacggatac
     2041 attgttccaa aggatgctca aattttggta aacttgtggg ccataggtag agatccaaat
     2101 gcttggcaaa atgccgatat tttctcacct gaaagattta taggttgtga gattgatgta
     2161 aagggaagag attttggttt attgcctttt ggtgctggta gaagaatttg ccctggtatg
     2221 aatttggcca taaggatgtt gacattgatg ttggccacat tattgcaatt cttcaactgg
     2281 aaattagagg gagacatttc acctaaagat ttggacatgg atgaaaagtt tggtatagcc
     2341 ttgcagaaaa ctaaaccttt aaaattgatc ccaattccaa gatacggatc cgtgagcgag
     2401 ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgaa caaccaccac
     2461 ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc
     2521 aaggcggtcg agggcggccc tctccccttc gccttcgaca tcctggctac cagcttcatg
     2581 tacggcagca aaaccttcat caaccacacc cagggcatcc ccgacttctt taagcagtcc
     2641 ttccccgagg gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc
     2701 gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg
     2761 gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggcctcc
     2821 accgaaaccc tgtaccccgc tgacggcggc ctggaaggca gagccgacat ggccctgaag
     2881 ctcgttggcg ggggccacct gatctgcaac ttgaagacca catacagatc caagaaaccc
     2941 gctaagaacc tcaagatgcc cggcgtctac tatgtggaca gaagactgga aagaatcaag
     3001 gaggccgaca aagaaaccta cgtcgagcag cacgaggtgg ctgtggccag atactgcgac
     3061 ctccctagca aactggggca cagataactc gaggcgaatt tcttatgatt tatgattttt
     3121 attattaaat aagttataaa aaaaataagt gtatacaaat tttaaagtga ctcttaggtt
     3181 ttaaaacgaa aattcttatt cttgagtaac tctttcctgt aggtcaggtt gctttctcag
     3241 gtatagcatg aggtcgctct tattgaccac acctctaccg gcatgccgag caaatgcctg
     3301 caaatcgctc cccatttcac tagtgccaca aggtcagggc actcatgcga caatcaactc
     3361 gatgcatgat ccgcaccatt gtcgaggggc cagcaataaa gctccacaca gtcggtcaat
     3421 agtgccgaag accacagacc cggttaagac atagccgaat ggagccgcgc cgaccacaga
     3481 atgatacaca gagagacgca cagctgcaga tcacgtgcta taaaaataat tataatttaa
     3541 attttttaat ataaatatat aaattaaaaa tagaaagtaa aaaaagaaat taaagaaaaa
     3601 atagtttttg ttttccgaag atgtaaaaga ctctaggggg atcgccaaca aatactacct
     3661 tttatcttgc tcttcctgct ctcaggtatt aatgccgaat tgtttcatct tgtctgtgta
     3721 gaagaccaca cacgaaaatc ctgtgatttt acattttact tatcgttaat cgaatgtata
     3781 tctatttaat ctgcttttct tgtctaataa atatatatgt aaagtacgct ttttgttgaa
     3841 attttttaaa cctttgttta tttttttttc ttcattccgt aactcttcta ccttctttat
     3901 ttactttcta aaatccaaat acaaaacata aaaataaata aacacagagt aaattcccaa
     3961 attattccat cattaaaaga tacgaggcgc gtgtaagtta caggcaagcg atccgtcatc
     4021 gactacgtcg ttaaggccgt ttctgacaga gtaaaattct tgagggaact ttcaccatta
     4081 tgggaaatgg ttcaagaagg tattgactta aactccatca aatggtcagg tcattgagtg
     4141 ttttttattt gttgtatttt ttttttttta gagaaaatcc tccaatatat aaattaggaa
     4201 tcatagtttc atgattttct gttacaccta actttttgtg tggtgccctc ctccttgtca
     4261 atattaatgt taaagtgcaa ttctttttcc ttatcacgtt gagccattag tatcaatttg
     4321 cttacctgta ttcctttaca tcctcctttt tctccttctt gataaatgta tgtagattgc
     4381 gtatatagtt tcgtctaccc tatgaacata ttccattttg taatttcgtg tcgtttctat
     4441 tatgaatttc atttataaag tttatgtaca aatatcataa aaaaagagaa tctttttaag
     4501 caaggatttt cttaacttct tcggcgacag catcaccgac ttcggtggta ctgttggaac
     4561 cacctaaatc accagttctg atacctgcat ccaaaacctt tttaactgca tcttcaatgg
     4621 ccttaccttc ttcaggcaag ttcaatgaca atttcaacat cattgcagca gacaagatag
     4681 tggcgatagg gttgacctta ttctttggca aatctggagc agaaccgtgg catggttcgt
     4741 acaaaccaaa tgcggtgttc ttgtctggca aagaggccaa ggacgcagat ggcaacaaac
     4801 ccaaggaacc tgggataacg gaggcttcat cggagatgat atcaccaaac atgttgctgg
     4861 tgattataat accatttagg tgggttgggt tcttaactag gatcatggcg gcagaatcaa
     4921 tcaattgatg ttgaaccttc aatgtaggaa attcgttctt gatggtttcc tccacagttt
     4981 ttctccataa tcttgaagag gccaaaacat tagctttatc caaggaccaa ataggcaatg
     5041 gtggctcatg ttgtagggcc atgaaagcgg ccattcttgt gattctttgc acttctggaa
     5101 cggtgtattg ttcactatcc caagcgacac catcaccatc gtcttccttt ctcttaccaa
     5161 agtaaatacc tcccactaat tctctgacaa caacgaagtc agtaccttta gcaaattgtg
     5221 gcttgattgg agataagtct aaaagagagt cggatgcaaa gttacatggt cttaagttgg
     5281 cgtacaattg aagttcttta cggattttta gtaaaccttg ttcaggtcta acactaccgg
     5341 ttccccattt aggaccaccc acagcaccta acaaaacggc atcagccttc ttggaggctt
     5401 ccagcgcctc atctggaagt ggaacacctg tagcatcgat agcagcacca ccaattaaat
     5461 gattttcgaa atcgaacttg acattggaac gaacatcaga aatagcttta agaaccttaa
     5521 tggcttcggc tgtgatttct tgaccaacgt ggtcacctgg caaaacgacg atcttcttag
     5581 gggcagacat tagaatggta tatccttgaa atatatatat atatattgct gaaatgtaaa
     5641 aggtaagaaa agttagaaag taagacgatt gctaaccacc tattggaaaa aacaataggt
     5701 ccttaaataa tattgtcaac ttcaagtatt gtgatgcaag catttagtca tgaacgcttc
     5761 tctattctat atgaaaagcc ggttccggcg ctctcacctt tcctttttct cccaattttt
     5821 cagttgaaaa aggtatatgc gtcaggcgac ctctgaaatt aacaaaaaat ttccagtcat
     5881 cgaatttgat tctgtgcgat agcgcccctg tgtgttctcg ttatgttgag gaaaaaaata
     5941 atggttgcta agagattcga actcttgcat cttacgatac ctgagtattc ccacagttaa
     6001 ctgcggtcaa gatatttctt gaatcaggcg ccttagaccg ctcggccaaa caaccaatta
     6061 cttgttgaga aatagagtat aattatccta taaatataac gtttttgaac acacatgaac
     6121 aaggaagtac aggacaattg attttgaaga gaatgtggat tttgatgtaa ttgttgggat
     6181 tccattttta ataaggcaat aatattaggt atgtagatat actagaagtt ctcctcgacg
     6241 gtcgggtacc ccgaacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     6301 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     6361 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     6421 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     6481 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     6541 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     6601 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     6661 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     6721 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     6781 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     6841 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     6901 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     6961 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgact
     7021 gatccttcaa ctcagcaaaa gttcgattta ttcaacaaag ccacgttgtg tctcaaaatc
     7081 tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt
     7141 acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctcca
     7201 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
     7261 atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt
     7321 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
     7381 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
     7441 atgatgcatg gttactcacc actgcgatcc ccgggaaaac agcattccag gtattagaag
     7501 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
     7561 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgc ctcgctcagg
     7621 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
     7681 gctggcctgt tgaacaagtc tggaaagaaa tgcataagct tttgccattc tcaccggatt
     7741 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
     7801 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     7861 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     7921 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
     7981 aatcagaatt ggttaattgg ttgtaacac
//
