pyEF1A vector (V001900)

LOCUS       V001900                 5324 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001900
VERSION     V001900
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5324)
  AUTHORS   Sorensen HP, Kristensen JE, Sperling-Petersen HU, Mortensen KK.
  TITLE     Soluble expression of aggregating proteins by covalent coupling to
            the ribosome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5324)
  AUTHORS   Sorensen HP, Sperling-Petersen HU, Mortensen KK.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2004) Department of Molecular Biology, Aarhus
            University, Gustav Wieds Vej 10C, Aarhus C 8000, Denmark
REFERENCE   3  (bases 1 to 5324)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5324)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (29-MAR-2004) Department of Molecular Biology, Aarhus University,
            Gustav Wieds Vej 10C, Aarhus C 8000, Denmark"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5324
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(78..734)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(735..837)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(1363..1907)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(2049..2924)
                     /label="araC"
                     /note="L-arabinose regulatory protein"
     promoter        2951..3235
                     /label="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite
                     direction (Guzman et al., 1995)"
     RBS             3262..3284
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3292..3591
                     /gene="rplW"
                     /label="Large ribosomal subunit protein uL23"
                     /note="Large ribosomal subunit protein uL23 from
                     Escherichia coli (strain K12). Accession#: P0ADZ0"
     CDS             3592..3603
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     CDS             3604..3624
                     /label="TEV site"
                     /note="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
     CDS             3628..5001
                     /gene="TEF1"
                     /label="Elongation factor 1-alpha"
                     /note="Elongation factor 1-alpha from Saccharomyces
                     cerevisiae (strain ATCC 204508 / S288c). Accession#:
                     P02994"
     CDS             5002..5019
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      5250..5296
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"