LOCUS       40924_48008        5324 bp DNA     circular SYN 18-DEC-2018
DEFINITION  RpL23-fusion expression vector pyEF1A, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5324)
  AUTHORS   Sorensen HP, Kristensen JE, Sperling-Petersen HU, Mortensen KK.
  TITLE     Soluble expression of aggregating proteins by covalent coupling to 
            the ribosome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5324)
  AUTHORS   Sorensen HP, Sperling-Petersen HU, Mortensen KK.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2004) Department of Molecular Biology, Aarhus 
            University, Gustav Wieds Vej 10C, Aarhus C 8000, Denmark
REFERENCE   3  (bases 1 to 5324)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5324)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-MAR-2004) Department of Molecular Biology, Aarhus University, 
            Gustav Wieds Vej 10C, Aarhus C 8000, Denmark"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5324
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(78..734)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(735..837)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(1363..1907)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(2049..2924)
                     /label=araC
                     /note="L-arabinose regulatory protein"
     promoter        2951..3235
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     RBS             3262..3284
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3292..3591
                     /gene="rplW"
                     /label=rplW
                     /note="Large ribosomal subunit protein uL23 from
                     Escherichia coli (strain K12). Accession#: P0ADZ0"
     CDS             3592..3603
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     CDS             3604..3624
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     CDS             3628..5001
                     /gene="TEF1"
                     /label=TEF1
                     /note="Elongation factor 1-alpha from Saccharomyces
                     cerevisiae (strain ATCC 204508 / S288c). Accession#: 
                     P02994"
     CDS             5002..5019
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      5250..5296
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 attcatccgc ttattatcac ttattcaggc gtagcaccag gcgtttaagg gcaccaataa
       61 ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta
      121 agcattctgc cgacatggaa gccatcacag acggcatgat gaacctgaat cgccagcggc
      181 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
      241 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag
      301 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
      361 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
      421 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
      481 catatcacca gctcaccgtc tttcattgcc atacggaatt ccggatgagc attcatcagg
      541 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
      601 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
      661 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
      721 atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
      781 cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaacg
      841 tctcattttc gccaaaagtt ggcccagggc ttcccggtat caacagggac accaggattt
      901 atttattctg cgaagtgatc ttccgtcaca ggtatttatt cggcgcaaag tgcgtcgggt
      961 gatgctgcca acttactgat ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc
     1021 tgtttctatc agctgtccct cctgttcagc tactgacggg gtggtgcgta acggcaaaag
     1081 caccgccgga catcagcgct agcggagtgt atactggctt actatgttgg cactgatgag
     1141 ggtgtcagtg aagtgcttca tgtggcagga gaaaaaaggc tgcaccggtg cgtcagcaga
     1201 atatgtgata caggatatat tccgcttcct cgctcactga ctcgctacgc tcggtcgttc
     1261 gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa gatgccagga
     1321 agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata ggctccgccc
     1381 ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact
     1441 ataaagatac caggcgtttc ccctggcggc tccctcgtgc gctctcctgt tcctgccttt
     1501 cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac gcctgacact
     1561 cagttccggg taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc
     1621 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa
     1681 agcaccactg gcagcagcca ctggtaattg atttagagga gttagtcttg aagtcatgcg
     1741 ccggttaagg ctaaactgaa aggacaagtt ttggtgactg cgctcctcca agccagttac
     1801 ctcggttcaa agagttggta gctcagagaa ccttcgaaaa accgccctgc aaggcggttt
     1861 tttcgttttc agagcaagag attacgcgca gaccaaaacg atctcaagaa gatcatctta
     1921 ttaatcagat aaaatatttc tagatttcag tgcaatttat ctcttcaaat gtagcacctg
     1981 aagtcagccc catacgatat aagttgtagc ggccgcaagc cgtcaattgt ctgattcgtt
     2041 accaattatg acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac
     2101 tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca
     2161 aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc
     2221 gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg
     2281 aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgatatca
     2341 aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc
     2401 atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc
     2461 agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc
     2521 ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg
     2581 gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc
     2641 cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat ctctcctggc
     2701 gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc
     2761 ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata
     2821 aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta
     2881 aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg
     2941 ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt
     3001 tactggctct tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa
     3061 agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa
     3121 gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata
     3181 agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg
     3241 tttttttggg ctagaaataa ttttgtttaa ctttaagaag gagatataca tatgattcgt
     3301 gaagaacgtc tgctgaaggt gctgcgtgca ccgcacgttt ctgaaaaagc gtctactgcg
     3361 atggaaaaat ccaacaccat cgtactcaaa gttgctaaag acgcgaccaa agcagaaatc
     3421 aaagctgctg tgcagaaact gtttgaagtc gaagtcgaag tcgttaacac cctggtagtt
     3481 aaagggaaag ttaaacgtca cggacagcgt atcggtcgtc gtagcgactg gaaaaaagct
     3541 tacgtcaccc tgaaagaagg ccagaatctg gacttcgttg gcggcgctga gatcgaaggt
     3601 cgtgagaatc tttattttca gggcgccatg ggtaaagaga agtctcacat taacgttgtc
     3661 gttatcggtc atgtcgattc tggtaagtct accactaccg gtcatttgat ttacaagtgt
     3721 ggtggtattg acaagagaac catcgaaaag ttcgaaaagg aagccgctga attaggtaag
     3781 ggttctttca agtacgcttg ggttttggac aagttaaagg ctgaaagaga aagaggtatc
     3841 actatcgata ttgctttgtg gaagttcgaa actccaaagt accaagttac cgttattgat
     3901 gctccaggtc acagagattt catcaagaac atgattactg gtacttctca agctgactgt
     3961 gctatcttga ttattgctgg tggtgtcggt gaattcgaag ccggtatctc taaggatggt
     4021 caaaccagag aacacgcttt gttggctttc accttgggtg ttagacaatt gattgttgct
     4081 gtcaacaaga tggactccgt caaatgggac gaatccagat tccaagaaat tgtcaaggaa
     4141 acctccaact ttatcaagaa ggttggttac aacccaaaga ctgttccatt cgtcccaatc
     4201 tctggttgga acggtgacaa catgattgaa gctaccacca acgctccatg gtacaagggt
     4261 tgggaaaagg aaaccaaggc cggtgtcgtc aagggtaaga ctttgttgga agccattgac
     4321 gccattgaac aaccatctag accaactgac aagccattga gattgccatt gcaagatgtt
     4381 tacaagattg gtggtattgg tactgtgcca gtcggtagag ttgaaaccgg tgtcatcaag
     4441 ccaggtatgg ttgttacttt tgccccagct ggtgttacca ctgaagtcaa gtccgttgaa
     4501 atgcatcacg aacaattgga acaaggtgtt ccaggtgaca acgttggttt caacgtcaag
     4561 aacgtttccg ttaaggaaat cagaagaggt aacgtctgtg gtgacgctaa gaacgatcca
     4621 ccaaagggtt gcgcttcttt caacgctacc gtcattgttt tgaaccatcc aggtcaaatc
     4681 tctgctggtt actctccagt tttggattgt cacactgctc acattgcttg tagattcgac
     4741 gaattgttgg aaaagaacga cagaagatct ggtaagaagt tggaagacca tccaaagttc
     4801 ttgaagtccg gtgacgctgc tttggtcaag ttcgttccat ctaagccaat gtgtgttgaa
     4861 gctttcagtg aatacccacc attaggtaga ttcgctgtca gagacatgag acaaactgtc
     4921 gctgtcggtg ttatcaagtc tgttgacaag actgaaaagg ccgctaaggt taccaaggct
     4981 gctcaaaagg ctgctaagaa acaccaccac caccaccact aataatacgt actcgagcat
     5041 gcaagcttgg ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag
     5101 aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac
     5161 ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtccc
     5221 ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tgcaaagact
     5281 gggcctttcg ttttatctgt tgtttgtcgg tgaacggcgg ccgc
//
