Basic Vector Information
pWALIUM10-moe vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pWALIUM10-moe vector Sequence
LOCUS 40924_46163 9842 bp DNA circular SYN 18-DEC-2018
DEFINITION Overexpression vector pWALIUM10-moe, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9842)
AUTHORS Ni J-Q., Liu L-P., Perkins LA, Perrimon N.
TITLE Vectors for transgenic flies
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 9842)
AUTHORS Ni J-Q., Liu L-P., Perkins LA, Perrimon N.
TITLE Direct Submission
JOURNAL Submitted (22-FEB-2010) Genetics, Harvard Medical School, 77 Avenue
Louis Pasteur, Boston, MA 02115, USA
REFERENCE 3 (bases 1 to 9842)
AUTHORS Hu Y, Perkins LA.
TITLE Direct Submission
JOURNAL Submitted (20-JUN-2012) Genetics, Harvard Medical School, 77 Avenue
Louis Pasteur, Boston, MA 02115, USA
REFERENCE 4 (bases 1 to 9842)
TITLE Direct Submission
REFERENCE 5 (bases 1 to 9842)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(22-FEB-2010) Genetics, Harvard Medical School, 77 Avenue Louis
Pasteur, Boston, MA 02115, USA"
COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
(20-JUN-2012) Genetics, Harvard Medical School, 77 Avenue Louis
Pasteur, Boston, MA 02115, USA"
COMMENT SGRef: number: 4; type: "Journal Article"
COMMENT On Jun 20, 2012 this sequence version replaced GU931385.1.
FEATURES Location/Qualifiers
source 1..9842
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(6..461)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 603..619
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 626..644
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
gene complement(682..4809)
/label=mini-white
/note="This modified version of the white gene lacks part
of the first intron."
protein_bind 4923..4992
/label=attB
/note="attB site for the phi-C31 integrase (Groth et al.,
2000)"
protein_bind 5230..5660
/label=gypsy insulator
/note="chromatin insulator from Drosophila"
protein_bind 5678..5711
/label=loxP
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
protein_bind 5724..5818
/label=5X UAS
/note="five tandem copies of the 'ScaI site' 17-mer
CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS)
that efficiently binds yeast Gal4 (Webster et al., 1988;
Pfeiffer et al., 2010)"
misc_recomb 5834..5867
/label=LoxP
/note="LoxP"
protein_bind complement(5834..5867)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
misc_feature 5874..5983
/label=5XUAS
/note="5XUAS"
protein_bind 5880..5974
/label=5X UAS
/bound_moiety="GAL4"
/note="five tandem copies of the ""ScaI site"" 17-mer
CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS)
that efficiently binds yeast Gal4 (Webster et al., 1988;
Pfeiffer et al., 2010)"
promoter 5998..6236
/label=hsp70 promoter
/note="Drosophila melanogaster hsp70Bb promoter"
misc_feature 6250..6291
/label=multiple cloning site
/note="multiple cloning site"
intron 6332..6478
/note="ftz intron"
intron 6578..6643
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 6773..6793
/label=SV40 NLS
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
polyA_signal 7065..7199
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
misc_feature 7208..7638
/label=gypsy insulator
/note="gypsy insulator"
protein_bind 7208..7638
/label=gypsy insulator
/bound_moiety="Su(Hw), CP190, and Mod(mdg4)67.2"
/note="chromatin insulator from Drosophila"
promoter complement(7657..7675)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(7696..7712)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(7720..7736)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7744..7774)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(7789..7810)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(8098..8686)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(8860..9717)
/label=AmpR
/note="beta-lactamase"
promoter complement(9718..9822)
/label=AmpR promoter
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