LOCUS       40924_46163        9842 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Overexpression vector pWALIUM10-moe, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9842)
  AUTHORS   Ni J-Q., Liu L-P., Perkins LA, Perrimon N.
  TITLE     Vectors for transgenic flies
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9842)
  AUTHORS   Ni J-Q., Liu L-P., Perkins LA, Perrimon N.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2010) Genetics, Harvard Medical School, 77 Avenue 
            Louis Pasteur, Boston, MA 02115, USA
REFERENCE   3  (bases 1 to 9842)
  AUTHORS   Hu Y, Perkins LA.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUN-2012) Genetics, Harvard Medical School, 77 Avenue 
            Louis Pasteur, Boston, MA 02115, USA
REFERENCE   4  (bases 1 to 9842)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 9842)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-FEB-2010) Genetics, Harvard Medical School, 77 Avenue Louis 
            Pasteur, Boston, MA 02115, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (20-JUN-2012) Genetics, Harvard Medical School, 77 Avenue Louis 
            Pasteur, Boston, MA 02115, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     On Jun 20, 2012 this sequence version replaced GU931385.1.
FEATURES             Location/Qualifiers
     source          1..9842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(6..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            complement(682..4809)
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     protein_bind    4923..4992
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     protein_bind    5230..5660
                     /label=gypsy insulator
                     /note="chromatin insulator from Drosophila"
     protein_bind    5678..5711
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    5724..5818
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     misc_recomb     5834..5867
                     /label=LoxP
                     /note="LoxP"
     protein_bind    complement(5834..5867)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    5874..5983
                     /label=5XUAS
                     /note="5XUAS"
     protein_bind    5880..5974
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        5998..6236
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     misc_feature    6250..6291
                     /label=multiple cloning site
                     /note="multiple cloning site"
     intron          6332..6478
                     /note="ftz intron"
     intron          6578..6643
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6773..6793
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    7065..7199
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    7208..7638
                     /label=gypsy insulator
                     /note="gypsy insulator"
     protein_bind    7208..7638
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, and Mod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     promoter        complement(7657..7675)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7696..7712)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7720..7736)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7744..7774)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7789..7810)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8098..8686)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8860..9717)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9718..9822)
                     /label=AmpR promoter
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtacaag
      661 cttcacgacc tgaggcgcgc ctccagtgaa atccaagcat tttctaaatt aaatgtattc
      721 ttattattat agttgttatt tttgatatat ataaacaaca ctattatgcc caccattttt
      781 ttgagatgca tctacacaag gaacaaacac tggatgtcac tttcagttca aattgtaacg
      841 ctaatcactc cgaacaggtc acaaaaaatt accttaaaaa gtcataatat taaattagaa
      901 taaatatagc tgtgagggaa atatatacaa atatattgga gcaaataaat tgtacataca
      961 aatatttatt actaatttct attgagacga aatgaaccac tcggaaccat ttgagcgaac
     1021 cgaatcgcgc ggaactaacg acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt
     1081 gcggagagcg ggtgggagac agcgaaagag caactacgaa acgtggtgtg gtggaggtga
     1141 attatgaaga gggcgcgcga tttgaaaagt atgtatataa aaaatatatc ccggtgtttt
     1201 atgtagcgat aaacgagttt ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga
     1261 agacaaatcc aaagtctact tgtggggatg ttcgaagggg aaatacttgt attctatagg
     1321 tcatatcttg tttttattgg cacaaatata attacattag ctttttgagg gggcaataaa
     1381 cagtaaacac gatggtaata atggtaaaaa aaaaaacaag cagttatttc ggatatatgt
     1441 cggctactcc ttgcgtcggg cccgaagtct tagagccaga tatgcgagca cccggaagct
     1501 cacgatgaga atggccagac ccacgtagtc cagcggcaga tcggcggcgg agaagttaag
     1561 cgtctccagg atgaccttgc ccgaactggg gcacgtggtg ttcgacgatg tgcagctaat
     1621 ttcgcccggc tccacgtccg cccattggtt aatcagcaga ccctcgttgg cgtaacggaa
     1681 ccatgagagg tacgacaacc atttgaggta tactggcacc gagcccgagt tcaagaagaa
     1741 gccgccaaag agcaggaatg gtatgataac cggcggaccc acagacagcg ccatcgaggt
     1801 cgaggagctg gcgcaggata ttagatatcc gaaggacgtt gacacattgg ccaccagagt
     1861 gaccagcgcc aggcagttga agaagtgcag cactccggcc cgcagtccga tcatcggata
     1921 ggcaatcgcc gtgaagacca gtggcactgt gagaaaaagc ggtaattcgg caatcgtttt
     1981 gcccagaaag tatgtgtcac agcgataaag tcgacttcgg gcctccctca taaaaactgg
     2041 cagctctgag gtgaacacct aaatcgaatc gattcattag aaagttagta aattattaat
     2101 atgcaaatgt attctaaaca agacttacat ttatcgtggc aaagacgttt tgaaaggtca
     2161 tgttggtcag gaagaggaag atggctccgt tgatattcat cacgcccact tgcgtgagtt
     2221 gttggcccaa aaagatgagg ccaatcaaga tggcaaccat ctgcaaatta aaatgttact
     2281 cgcatctcat taatattcat atcttcaaca tgttcgcgag ttaaatgaaa tttatttatt
     2341 ttctgcaaaa ctataaacta tacatctcat tgaaaaaaac taagaagggt gtggaatcag
     2401 gcaattctaa ctaaaatcta gcgaatttgt ttccaagaat tgtaagcgtt atatcatttg
     2461 tttccactgg aaccactcac cgttgtctga ataagtcgca cttttacgag gagtggttcc
     2521 ttgagcaccg acagccagga tcgccacagg accgcccgga actgcatgaa ccaggtggcc
     2581 ttgtaggtgt acccattctc cggctgctcc agtggcttct ccaaattttt ggtggccaac
     2641 aactgctcca tatcccgggc tactttgcta atagcaaaat tgtcgcatat cttggcgatc
     2701 cgatcacggg actcgatctc ccgtccgggc acaacggcca acacctgtac gtaaaagtcc
     2761 gccggattgt agttggtagg acactgggca cccacgctgg ataggagttg agatgtaatg
     2821 taatgctaga tacccttaat aaacacatcg aactcactag gaaaagaagt cgacggcttc
     2881 gctgggagtg cccaagaaag ctaccctgcc ctcggccatc agaaggatct tgtcaaagag
     2941 ctcaaacagc tcggaagacg gctgatgaat ggtcaggatg acggtcttgc ccttctgcga
     3001 cagcttcttc agcacctgga cgacgctgtg ggcggtaaat gagtccagtc cggaggtggg
     3061 ctcatcgcag atcagaagcg gcggatcggt tagtgcctcg gaggcgaatg ccagacgctt
     3121 cctttctccg ccggacagac ctttcaccct gccgggcaca ccgatgatcg tgtgctgaca
     3181 tttgctgagc gaaagctcct ggatcacctg atccacgcgg gccactcgct gccgataggt
     3241 cagatgtcgt ggcatccgca ccatggcctg gaaaatcagg tgttccctgg ccgttaggga
     3301 gccgataaag aggtcatcct gctggacata ggcgcacctg gcctgcatct ccttggcgtc
     3361 cacaggttgg ccattgagca gtcgcatccc ggatggcgat acttggatgc cctgcggcga
     3421 tcgaaaggca agggcattca gcagggtcgt ctttccggca ccggaactgc ccatcacggc
     3481 caaaagttcg cccggatagg ccacgccgca aactgagttt caaattggta attggaccct
     3541 ttattaagat ttcacacaga tcagccgact gcgaatagaa actcaccgtt cttgagcaaa
     3601 tgtttcctgg gcgccggtat gtgtcgctcg ttgcagaata gtccgcgtgt ccggttgacc
     3661 agctgccgcc atccggagcc cggctgattg accgccccaa agatgtccat attgtgccag
     3721 gcataggtga ggttctcggc tagttggccg ctccctgaac cggagtcctc cggcggactg
     3781 ggtggcagga gcgtgccgta gtttttggcc tgcccgaagc cctggttaat gcagctctgc
     3841 gaagccgctc cgctgtcacc ctgcaatgat aggggatctc aaatatcaac tacaagcgtt
     3901 atgctcatct aaccccgaac aaaacgaagt atcctacgaa gtaggtttat acttttattt
     3961 attttttgtg catctaggat cagcttaaaa tatctggttg ttatattttt tgtaaaaaag
     4021 aatgtagtcg aaaatgaatg cctttagatg tcttgatcat gatatgatct taaaaattgt
     4081 cttatatagc gagcacagct accagaataa tctgtttcgt gtcactattt gtttgtgcga
     4141 ttgcggtttg ggatttttgt gggtcgcagt tctcacgccg cagacaattt gatgttgcaa
     4201 tcgcagttcc tatagatcaa gtgaacttaa gatgtatgca catgtactac tcacattgtt
     4261 cagatgctcg gcagatgggt gtttgctgcc tccgcgaatt aatagctcct gatcctcttg
     4321 gcccattgcc gggatttttc acactttccc ctgcttaccc acccaaaacc aatcaccacc
     4381 ccaatcactc aaaaaacaaa caaaaataag aagcgagagg agttttggca cagcactttg
     4441 tgtttaattg atggcgtaaa ccgcttggag cttcgtcacg aaaccgctga caaagtgcaa
     4501 ctgaaggcgg acattgacgc taggtaacgc tacaaacggt ggcgaaagag atagcggacg
     4561 cagcggcgaa agagacggcg atatttctgt ggacagagaa ggaggcaaac agcgctgact
     4621 ttgagtggaa tgtcattttg agtgagaggt aatcgaaaga acctggtact tcaaataccc
     4681 ttggatcgaa gtaaatttaa aactgatcag ataagttcaa tgatatccag tgcagtaaaa
     4741 aataaaaaaa aaaaatgttt ttttttatct actttccgca aaaatgggtt ttattaactt
     4801 acatacatgg cgcgccagat cgcaagaagc ttgatatcat cgatctcgag gctgcatcca
     4861 acgcgttggg agctctccgg atcaattcgg cttcaggtac cgtcgacgat gtaggtcacg
     4921 gtctcgaagc cgcggtgcgg gtgccagggc gtgcccttgg gctccccggg cgcgtactcc
     4981 acctcaccca tctggtccat catgatgaac gggtcgaggt ggcggtagtt gatcccggcg
     5041 aacgcgcggc gcaccgggaa gccctcgccc tcgaaaccgc tgggcgcggt ggtcacggtg
     5101 agcacgggac gtgcgacggc gtcggcgggt gcggatacgc ggggcagcgt cagcgggttc
     5161 tcgacggtca cggcgggcat gtcgacaagc cgaattgatc cactagaagg cctaattcgg
     5221 tacactagtt ggccacgtaa taagtgtgcg ttgaatttat tcgcaaaaac attgcatatt
     5281 ttcggcaaag taaaattttg ttgcatacct tatcaaaaaa taagtgctgc atacttttta
     5341 gagaaaccaa ataatttttt attgcatacc cgtttttaat aaaatacatt gcataccctc
     5401 ttttaataaa aaatattgca tactttgacg aaacaaattt tcgttgcata cccaataaaa
     5461 gattattata ttgcataccc gtttttaata aaatacattg cataccctct tttaataaaa
     5521 aatattgcat acgttgacga aacaaatttt cgttgcatac ccaataaaag attattatat
     5581 tgcatacctt ttcttgccat accatttagc cgatcaattg tgctcggcaa cagtatattt
     5641 gtggtgtgcc aaccaacaac actagtagta ccagcttata acttcgtata atgtatgcta
     5701 tacgaagtta tctgcaggca ggtcggagta ctgtcctccg agcggagtac tgtcctccga
     5761 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
     5821 gagactccca tggataactt cgtataatgt atgctatacg aagttatgga tccgcaggtc
     5881 ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga
     5941 gtactgtcct ccgagcggag tactgtcctc cgagcggaga ctcgtcgaca gcgagcgccg
     6001 gagtataaat agaggcgctt cgtctacgga gcgacaattc aattcaaaca agcaaagtga
     6061 acacgtcgct aagcgaaagc taagcaaata aacaagcgca gctgaacaag ctaaacaatc
     6121 tgcagtaaag tgcaagttaa agtgaatcaa ttaaaagtaa ccagcaacca agtaaatcaa
     6181 ctgcaactac tgaaatctgc caagaagtaa ttattgaata caagaagaga actctgaata
     6241 gggaattggg aattcgcaga tctccatatg agctagctac tagtgtctag agcaaactag
     6301 ttctgatctg ctagacaatt gttggcatca ggtaggcatc acacacgatt aacaacccct
     6361 aaaaatacac tttgaaaata ttgaaaatat gtttttgtat acatttttga tattttcaaa
     6421 caatacgcag ttataaaact cattagctaa cccatttttt ctttgcttat gcttacagat
     6481 tgcaaagaac tagagccgcg ggatctttgt gaaggaacct tacttctgtg gtgtgacata
     6541 attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt
     6601 ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac
     6661 tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga
     6721 aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa
     6781 gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca
     6841 tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc
     6901 tgcactgcta tacaagaaaa ttatggaaaa atatttgatg tatagtgcct tgactagaga
     6961 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     7021 tccccctgaa cctgaaacat aaaatgaatg gaattgttgt tgttaacttg tttattgcag
     7081 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     7141 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggttcc
     7201 agagctctgg ccacgtaata agtgtgcgtt gaatttattc gcaaaaacat tgcatatttt
     7261 cggcaaagta aaattttgtt gcatacctta tcaaaaaata agtgctgcat actttttaga
     7321 gaaaccaaat aattttttat tgcatacccg tttttaataa aatacattgc ataccctctt
     7381 ttaataaaaa atattgcata ctttgacgaa acaaattttc gttgcatacc caataaaaga
     7441 ttattatatt gcatacccgt ttttaataaa atacattgca taccctcttt taataaaaaa
     7501 tattgcatac gttgacgaaa caaattttcg ttgcataccc aataaaagat tattatattg
     7561 catacctttt cttgccatac catttagccg atcaattgtg ctcggcaaca gtatatttgt
     7621 ggtgtgccaa ccaacaacga gctccagctt ttgttccctt tagtgagggt taatttcgag
     7681 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     7741 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     7801 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     7861 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     7921 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     7981 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8041 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8101 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8161 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8221 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8281 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8341 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8401 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     8461 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     8521 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     8581 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     8641 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     8701 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     8761 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     8821 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     8881 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     8941 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9001 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9061 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9121 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9181 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9241 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9301 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9361 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9421 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     9481 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     9541 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     9601 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     9661 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     9721 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     9781 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     9841 gc
//
