Basic Vector Information
- Vector Name:
- pW1336
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6628 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Host:
- Yeast
- Source/Author:
- Barrett SP, Wang PL, Salzman J.
- Promoter:
- LEU2
pW1336 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pW1336 vector Sequence
LOCUS 40924_46158 6628 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pW1336, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6628)
AUTHORS Barrett SP, Wang PL, Salzman J.
TITLE Circular RNA biogenesis can proceed through an exon-containing
lariat precursor
JOURNAL Elife 4 (2015) In press
PUBMED 26057830
REFERENCE 2 (bases 1 to 6628)
AUTHORS Wang PL.
TITLE Direct Submission
JOURNAL Submitted (08-JUN-2015) Biochemistry, Stanford University, 279
Campus Drive, Stanford, CA 94305, USA
REFERENCE 3 (bases 1 to 6628)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 6628)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Elife 4
(2015) In press"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(08-JUN-2015) Biochemistry, Stanford University, 279 Campus Drive,
Stanford, CA 94305, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6628
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(147..930)
/direction=LEFT
/label=ars1
/note="Schizosaccharomyces pombe autonomously replicating
sequence ars1"
primer_bind complement(1280..1296)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 1509..1964
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2246..2350
/label=AmpR promoter
CDS 2351..3208
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3382..3970
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 4258..4279
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 4294..4324
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4332..4348
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 4356..4372
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
CDS complement(4876..5967)
/codon_start=1
/label=LEU2
/note="3-isopropylmalate dehydrogenase, required for
leucine biosynthesis"
/translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
DLGGSNSTTEVGDAVAEEVKKILA"
promoter complement(5968..6375)
/label=LEU2 promoter
This page is informational only.