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pW1336 vector

Basic Vector Information

Basic Vector Information
Vector Name pW1336 Antibiotic Resistance Ampicillin
Length 6628 bp Type Cloning vector
Source Barrett SP, Wang PL, Salzman J.

pW1336 vector Vector Map

pW1336 vector Sequence

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LOCUS       Exported                6628 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pW1336, complete sequence.
ACCESSION   KT033769
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6628)
  AUTHORS   Barrett SP, Wang PL, Salzman J.
  TITLE     Circular RNA biogenesis can proceed through an exon-containing 
            lariat precursor
  JOURNAL   Elife 4 (2015) In press
  PUBMED    26057830
REFERENCE   2  (bases 1 to 6628)
  AUTHORS   Wang PL.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2015) Biochemistry, Stanford University, 279 
            Campus Drive, Stanford, CA 94305, USA
REFERENCE   3  (bases 1 to 6628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 18, 2018 from SnapGene 4.1.8
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6628
                     /organism="Cloning vector pW1336"
                     /mol_type="other DNA"
                     /db_xref="taxon:1670968"
     rep_origin      74..1279
                     /label=ars1
                     /note="ars1"
     rep_origin      147..930
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(1280..1296)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1491..1966
                     /label=M13 origin
                     /note="M13 origin"
     rep_origin      1509..1964
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2246..2350
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2351..3211
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="AKM50016.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3382..3970
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4258..4279
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4294..4324
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4332..4348
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4356..4372
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            complement(4385..6622)
                     /gene="LEU2"
                     /label=LEU2
     CDS             complement(4873..5967)
                     /codon_start=1
                     /gene="LEU2"
                     /product="beta-isopropylmalate dehydrogenase"
                     /label=LEU2
                     /note="derived from Saccharomyces cerevisiae"
                     /protein_id="AKM50017.1"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(5968..6375)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     misc_feature    6623..73
                     /note="multiple cloning site: SphI, SpeI, SfiI, NcoI, AscI,
                     PacI, NotI, XhoI, SacI"

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