LOCUS 40924_46158 6628 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pW1336, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6628) AUTHORS Barrett SP, Wang PL, Salzman J. TITLE Circular RNA biogenesis can proceed through an exon-containing lariat precursor JOURNAL Elife 4 (2015) In press PUBMED 26057830 REFERENCE 2 (bases 1 to 6628) AUTHORS Wang PL. TITLE Direct Submission JOURNAL Submitted (08-JUN-2015) Biochemistry, Stanford University, 279 Campus Drive, Stanford, CA 94305, USA REFERENCE 3 (bases 1 to 6628) TITLE Direct Submission REFERENCE 4 (bases 1 to 6628) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Elife 4 (2015) In press" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (08-JUN-2015) Biochemistry, Stanford University, 279 Campus Drive, Stanford, CA 94305, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..6628 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin complement(147..930) /direction=LEFT /label=ars1 /note="Schizosaccharomyces pombe autonomously replicating sequence ars1" primer_bind complement(1280..1296) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin 1509..1964 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 2246..2350 /label=AmpR promoter CDS 2351..3208 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 3382..3970 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 4258..4279 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 4294..4324 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4332..4348 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 4356..4372 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" CDS complement(4876..5967) /codon_start=1 /label=LEU2 /note="3-isopropylmalate dehydrogenase, required for leucine biosynthesis" /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG DLGGSNSTTEVGDAVAEEVKKILA" promoter complement(5968..6375) /label=LEU2 promoter ORIGIN 1 ttactagtat ggcccagccg gccatggttg gcgcgccact taattaactg cggccgcaac 61 tcgagatgag ctcgaattcg agtctaactc cttaaccact cggacatagt gacttatctg 121 acactcattg taaattaata tatatatagg cattttgttt agttaaaggt acttaagtaa 181 ttagtataaa cgaaccaatt ttataatcag gaagttaagt gaatggtagc acatgtcgta 241 aaaattgtga atttttattg aataatattt taaatacaag cctttctaga ctaggtatac 301 tcataaacat atatgagcaa aaggatagag gagattacat tgcatcttct acaaatttat 361 ttattgccct ttactgaaaa attaaataat gagtactaaa tgataaaaag cgctcagtac 421 aagaaagatt gcaaaaatat tgcattcttc atgaattaaa gttgcatata aggcatattg 481 aaagtaatag tactaaaaca gcagttagcg aaaattaata gaattatatt cgcaagacaa 541 ttgtgacatt aaattaaaaa attgtagaat ttttactatc ctctttaacg ccatgagcct 601 ttataaaaag gttaaattag ttttaacatt ctttttttga gtaagagttt acaatttatc 661 aaaacctgtg ttattatatt cattagtttc aatttattag catctagaga aaaatcaatt 721 ggcagttaca ttgttggaat ttatgaagaa aaagaatcta caacggagaa taagttgctg 781 atcgctttcc ctaaaattgt atattttgct gagcttattt tgacatttcg ttgaagtttt 841 ctctaattcg cattcatttt aagtaaaaca atgagaaata aaattacaaa aaatacaaat 901 taaaatacaa tttttagcta taattataga cgatgccctt gtatcccatt ctgtctcgct 961 tgcccctact ttttatcttt tatataccat aatgaacgct gccgctacta accatacccc 1021 gattttacat ttcggactcc caaggacgta caaaatagaa aactatagaa aaaaataatc 1081 agaaaatagc atgtcatctc tttgtaaaac gcgtttgcaa gaagaaagga aacaatggag 1141 aagagatcat ccatttgtat gtaaatttta gtaaacttga agaaatcact aacaacttct 1201 cttacttagg gattctatgc aaaaccttgt aaatcatctg atggaggact cgatttaatg 1261 aattggaagg ttggaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg 1321 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg 1381 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc 1441 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca 1501 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag 1561 cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt 1621 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt 1681 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg 1741 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt 1801 taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt 1861 tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca 1921 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag 1981 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga 2041 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc 2101 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg 2161 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc 2221 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 2281 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 2341 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt 2401 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca 2461 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag 2521 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc 2581 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca 2641 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt 2701 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct 2761 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt 2821 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga 2881 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact 2941 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc 3001 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga 3061 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt 3121 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga 3181 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact 3241 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga 3301 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 3361 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 3421 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 3481 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta 3541 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 3601 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 3661 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 3721 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 3781 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 3841 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 3901 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 3961 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 4021 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt 4081 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga 4141 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta 4201 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa 4261 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat 4321 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta 4381 cgccaagctt gtcgatcgac tacgtcgtta aggccgtttc tgacagagta aaattcttga 4441 gggaactttc accattatgg gaaatggttc aagaaggtat tgacttaaac tccatcaaat 4501 ggtcaggtca ttgagtgttt tttatttgtt gtattttttt ttttttagag aaaatcctcc 4561 aatatataaa ttaggaatca tagtttcatg attttctgtt acacctaact ttttgtgtgg 4621 tgccctcctc cttgtcaata ttaatgttaa agtgcaattc tttttcctta tcacgttgag 4681 ccattagtat caatttgctt acctgtattc ctttacatcc tcctttttct ccttcttgat 4741 aaatgtatgt agattgcgta tatagtttcg tctaccctat gaacatattc cattttgtaa 4801 tttcgtgtcg tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa 4861 aagagaatct ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc 4921 ggtggtactg ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt 4981 aactgcatct tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat 5041 tgcagcagac aagatagtgg cgatagggtt gaccttattc tttggcaaat ctggagcaga 5101 accgtggcat ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga 5161 cgcagatggc aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc 5221 accaaacatg ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat 5281 catggcggca gaatcaatca attgatgttg aaccttcaat gtagggaatt cgttcttgat 5341 ggtttcctcc acagtttttc tccataatct tgaagaggcc aaaacattag ctttatccaa 5401 ggaccaaata ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat 5461 tctttgcact tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc 5521 ttcctttctc ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt 5581 acctttagca aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt 5641 acatggtctt aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc 5701 aggtctaaca ctaccggtac cccatttagg accacccaca gcacctaaca aaacggcatc 5761 aaccttcttg gaggcttcca gcgcctcatc tggaagtggg acacctgtag catcgatagc 5821 agcaccacca attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat 5881 agctttaaga accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa 5941 aacgacgatc ttcttagggg cagacattag aatggtatat ccttgaaata tatatatata 6001 tattgctgaa atgtaaaagg taagaaaagt tagaaagtaa gacgattgct aaccacctat 6061 tggaaaaaac aataggtcct taaataatat tgtcaacttc aagtattgtg atgcaagcat 6121 ttagtcatga acgcttctct attctatatg aaaagccggt tccgcggctc tcacctttcc 6181 tttttctccc aatttttcag ttgaaaaagg tatatgcgtc aggcgacctc tgaaattaac 6241 aaaaaatttc cagtcatcga atttgattct gtgcgatagc gcccctgtgt gttctcgtta 6301 tgttgaggaa aaaaataatg gttgctaaga gattcgaact cttgcatctt acgatacctg 6361 agtattccca cagttaactg cggtcaagat atttcttgaa tcaggcgcct tagaccgctc 6421 ggccaaacaa ccaattactt gttgagaaat agagtataat tatcctataa atataacgtt 6481 tttgaacaca catgaacaag gaagtacagg acaattgatt ttgaagagaa tgtggatttt 6541 gatgtaattg ttgggattcc atttttaata aggcaataat attaggtatg tagatatact 6601 agaagttctc ctcgacaagc ttgcatgc //