Basic Vector Information
- Vector Name:
- pVSR326
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9152 bp
- Type:
- Chloroplast transformation vector
- Replication origin:
- ori
- Source/Author:
- Reddy VS, Giovanni F, Leelavathi S.
pVSR326 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pVSR326 vector Sequence
LOCUS 40924_46123 9152 bp DNA circular SYN 18-DEC-2018
DEFINITION Chloroplast transformation vector pVSR326, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9152)
AUTHORS Reddy VS, Giovanni F, Leelavathi S.
TITLE Chloroplast transformation vector pVSR326
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 9152)
AUTHORS Reddy VS, Giovanni F, Leelavathi S.
TITLE Direct Submission
JOURNAL Submitted (08-JUL-2002) Plant Transformation Group, International
Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali
Marg, New Delhi, Delhi 110067, India
REFERENCE 3 (bases 1 to 9152)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 9152)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(08-JUL-2002) Plant Transformation Group, International Center for
Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New
Delhi, Delhi 110067, India"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..9152
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 379..395
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 405..1636
/note="chloroplast targeting sequence 1; derived from
tobacco"
regulatory 1661..2132
/note="psbA promoter; derived from rice"
/regulatory_class="promoter"
CDS 2133..3941
/label=GUS
/note="beta-glucuronidase"
regulatory 4014..4387
/note="psbA terminator; derived from rice"
/regulatory_class="terminator"
regulatory 4393..4503
/note="rrn promoter; derived from rice"
/regulatory_class="promoter"
CDS 4533..5318
/label=SmR
/note="aminoglycoside adenylyltransferase (Murphy, 1985)"
regulatory 5326..5583
/note="rbcL terminator; derived from rice"
/regulatory_class="terminator"
misc_feature 5598..6914
/note="chloroplast targeting sequence 2; derived from
tobacco"
primer_bind complement(6931..6947)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(6955..6971)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6979..7009)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(7024..7045)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(7333..7921)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(8095..8952)
/label=AmpR
/note="beta-lactamase"
promoter complement(8953..9057)
/label=AmpR promoter
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