LOCUS       40924_46123        9152 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Chloroplast transformation vector pVSR326, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9152)
  AUTHORS   Reddy VS, Giovanni F, Leelavathi S.
  TITLE     Chloroplast transformation vector pVSR326
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9152)
  AUTHORS   Reddy VS, Giovanni F, Leelavathi S.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2002) Plant Transformation Group, International 
            Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali 
            Marg, New Delhi, Delhi 110067, India
REFERENCE   3  (bases 1 to 9152)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9152)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-JUL-2002) Plant Transformation Group, International Center for 
            Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New 
            Delhi, Delhi 110067, India"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    405..1636
                     /note="chloroplast targeting sequence 1; derived from
                     tobacco"
     regulatory      1661..2132
                     /note="psbA promoter; derived from rice"
                     /regulatory_class="promoter"
     CDS             2133..3941
                     /label=GUS
                     /note="beta-glucuronidase"
     regulatory      4014..4387
                     /note="psbA terminator; derived from rice"
                     /regulatory_class="terminator"
     regulatory      4393..4503
                     /note="rrn promoter; derived from rice"
                     /regulatory_class="promoter"
     CDS             4533..5318
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     regulatory      5326..5583
                     /note="rbcL terminator; derived from rice"
                     /regulatory_class="terminator"
     misc_feature    5598..6914
                     /note="chloroplast targeting sequence 2; derived from
                     tobacco"
     primer_bind     complement(6931..6947)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6955..6971)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6979..7009)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7024..7045)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7333..7921)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8095..8952)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8953..9057)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgaaaga gataaattga
      421 acaagtatgg tcgtcccctg ttgggatgta ctattaaacc taaattgggg ttatctgcta
      481 aaaactacgg tagagccgtt tatgaatgtc ttcgcggtgg acttgatttt actaaagatg
      541 atgagaacgt gaactcacaa ccatttatgc gttggagaga tcgtttctta ttttgtgccg
      601 aagcacttta taaagcacag gctgaaacag gtgaaatcaa agggcattac ttgaatgcta
      661 ctgcaggtac atgcgaagaa atgatcaaaa gagctgtatt tgctagagaa ttgggcgttc
      721 cgatcgtaat gcatgactac ttaacggggg gattcaccgc aaatactagc ttggctcatt
      781 attgccgaga taatggtcta cttcttcaca tccaccgtgc aatgcatgcg gttattgata
      841 gacagaagaa tcatggtatc cacttccggg tattagcaaa agcgttacgt atgtctggtg
      901 gagatcatat tcactctggt accgtagtag gtaaacttga aggtgaaaga gacataactt
      961 tgggctttgt tgatttactg cgtgatgatt ttgttgaaca agatcgaagt cgcggtattt
     1021 atttcactca agattgggtc tctttaccag gtgttctacc cgtggcttca ggaggtattc
     1081 acgtttggca tatgcctgct ctgaccgaga tctttgggga tgattccgta ctacagttcg
     1141 gtggaggaac tttaggacat ccttggggta atgcgccagg tgccgtagct aatcgagtag
     1201 ctctagaagc atgtgtaaaa gctcgtaatg aaggacgtga tcttgctcag gaaggtaatg
     1261 aaattattcg cgaggcttgc aaatggagcc cggaactagc tgctgcttgt gaagtatgga
     1321 aagagatcgt atttaatttt gcagcagtgg acgttttgga taagtaaaaa cagtagacat
     1381 tagcagataa attagcagga aataaagaag gataaggaga aagaactcaa gtaattatcc
     1441 ttcgttctct taattgaatt gcaattaaac tcggcccaat cttttactaa aaggattgag
     1501 ccgaatacaa caaagattct attgcatata ttttgactaa gtatatactt acctagatat
     1561 acaagatttg aaatacaaaa tctagaaaac taaatcaaaa tctaagactc aaatctttct
     1621 attgttgtct tggatcatcg agcttgcatg cctgcagtcg actttcacag tttccattct
     1681 gaaatgttct ctgtactata ataaatagta agtgaatcaa cttactacta aaaaaattag
     1741 tagacttcct cttcggaata gaaatagcct atttctacat agggaaagtc gtgtgcaatg
     1801 aaaaatgcaa gcacgatttg gggagaggtt ttttctctat tgtaacaagg aataattatc
     1861 tactccatcc gactagttcc gggttcgagt cccgggcaac ccatatggaa actagaaagg
     1921 agcaatctga gttttgattt ttcactcact tcatttacaa aattttttgg tttggtaaat
     1981 tttgttgtat ggatatacaa ctgtcggggc tggcttggtt gacattggta tatagtctat
     2041 attatactgt taaataacaa gccttctatt atctttctag ttaatacgtg tgcttgggag
     2101 tccttgcaat ttgaataaac caagatctta ccatggtccg tcctgtagaa accccaaccc
     2161 gtgaaatcaa aaaactcgac ggcctgtggg cattcagtct ggatcgcgaa aactgtggaa
     2221 ttgatcagcg ttggtgggaa agcgcgttac aagaaagccg ggcaattgct gtgccaggca
     2281 gttttaacga tcagttcgcc gatgcagata ttcgtaatta tgcgggcaac gtctggtatc
     2341 agcgcgaagt ctttataccg aaaggttggg caggccagcg tatcgtgctg cgtttcgatg
     2401 cggtcactca ttacggcaaa gtgtgggtca ataatcagga agtgatggag catcagggcg
     2461 gctatacgcc atttgaagcc gatgtcacgc cgtatgttat tgccgggaaa agtgtacgta
     2521 tcaccgtttg tgtgaacaac gaactgaact ggcagactat cccgccggga atggtgatta
     2581 ccgacgaaaa cggcaagaaa aagcagtctt acttccatga tttctttaac tatgccggaa
     2641 tccatcgcag cgtaatgctc tacaccacgc cgaacacctg ggtggacgat atcaccgtgg
     2701 tgacgcatgt cgcgcaagac tgtaaccacg cgtctgttga ctggcaggtg gtggccaatg
     2761 gtgatgtcag cgttgaactg cgtgatgcgg atcaacaggt ggttgcaact ggacaaggca
     2821 ctagcgggac tttgcaagtg gtgaatccgc acctctggca accgggtgaa ggttatctct
     2881 atgaactgtg cgtcacagcc aaaagccaga cagagtgtga tatctacccg cttcgcgtcg
     2941 gcatccggtc agtggcagtg aagggcgaac agttcctgat taaccacaaa ccgttctact
     3001 ttactggctt tggtcgtcat gaagatgcgg acttgcgtgg caaaggattc gataacgtgc
     3061 tgatggtgca cgaccacgca ttaatggact ggattggggc caactcctac cgtacctcgc
     3121 attaccctta cgctgaagag atgctcgact gggcagatga acatggcatc gtggtgattg
     3181 atgaaactgc tgctgtcggc ttttcgctct ctttaggcat tggtttcgaa gcgggcaaca
     3241 agccgaaaga actgtacagc gaagaggcag tcaacgggga aactcagcaa gcgcacttac
     3301 aggcgattaa agagctgata gcgcgtgaca aaaaccaccc aagcgtggtg atgtggagta
     3361 ttgccaacga accggatacc cgtccgcaag gtgcacggga atatttcgcg ccactggcgg
     3421 aagcaacgcg taaactcgac ccgacgcgtc cgatcacctg cgtcaatgta atgttctgcg
     3481 acgctcacac cgataccatc agcgatctct ttgatgtgct gtgcctgaac cgttattacg
     3541 gatggtatgt ccaaagcggc gatttggaaa cggcagagaa ggtactggaa aaagaacttc
     3601 tggcctggca ggagaaactg catcagccga ttatcatcac cgaatacggc gtggatacgt
     3661 tagccgggct gcactcaatg tacaccgaca tgtggagtga agagtatcag tgtgcatggc
     3721 tggatatgta tcaccgcgtc tttgatcgcg tcagcgccgt cgtcggtgaa caggtatgga
     3781 atttcgccga ttttgcgacc tcgcaaggca tattgcgcgt tggcggtaac aagaaaggga
     3841 tcttcactcg cgaccgcaaa ccgaagtcgg cggcttttct gctgcaaaaa cgctggactg
     3901 gcatgaactt cggtgaaaaa ccgcagcagg gaggcaaaca atgaatcaac aactctcctg
     3961 gcgcaccatc gtcggctaca gcctcggtgg ggaattgagc tctaattaat taaggctttt
     4021 ctgctaacat atagcaattt ttgaagaaag gaaagctaga aatacccaat atcttgctga
     4081 agcaagatat tgggtatttc tttttttttt attttgaatc tttctattct gaattcagtt
     4141 aacgacgaga tttagtatcc tttcttgcac tttcataact cgtgaaatgc cgagttggta
     4201 cgaattcccc caatttgcga cctaccatag gatttgttat gtaaataggt atatgttcct
     4261 ttccattatg aatcgcgatt gtatggccaa ccattgcggg tagaatgcta gatgcccggg
     4321 accacgttac tattgtttct ttctcctcct tcatattgag cttttctatt tttgccaata
     4381 aatgatggta cctccccccg ccacgatcga acgggaatgg ataagaggct tgtgggattg
     4441 acgtgatagg gtagggttgg ctatactgct ggtggcgaac tccaggctaa taatctgaag
     4501 cgcttggaag ttgtagggag gatcctatgg ctcgtgaagc ggttatcgcc gaagtatcga
     4561 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     4621 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     4681 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     4741 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     4801 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     4861 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     4921 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     4981 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     5041 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     5101 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     5161 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     5221 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     5281 actacgtgaa aggcgagatc actaaggtag ttggcaaata actcgagact aagtggataa
     5341 aattagatag aaaaaaggtc taaataaaaa agaagagaaa tagaaagatc aaaaatcagt
     5401 tacgaaaatg cagtaattct tcttttttct tctaattgat tgcaattaaa ctcgtctcaa
     5461 tctgaaaaaa gattgagccg agtttaaata gattttgata cgatcatgag acttgacaaa
     5521 tcgggattcc tctattctat atatttagaa gatataaagg tataatacaa taaataaata
     5581 caatctagaa gctcgatgat ccttaggatt ggtatattct tttctatcct gtagtttgta
     5641 gtttccctga atcaagccaa gtatcacacc tctttctacc catcctgtat attgtcccct
     5701 ttgttccgtg ttgaaataga accttaattt attacttatt tttttattaa attttagatt
     5761 tgttagtgat tagatattag tattagacga gattttacga aacaattatt tttttatttc
     5821 tttataggag aggacaaatc tcttttttcg atgcgaattt gacacgacat aggagaagcc
     5881 gccctttatt aaaaattata ttattttaaa taatataaag ggggttccaa catattaata
     5941 tatagtgaag tgttccccca gattcagaac tttttttcaa tactcacaat ccttattagt
     6001 taataatcct agtgattgga tttctatgct tagtctgata ggaaataaga tattcaaata
     6061 aataatttta tagcgaatga ctattcatct attgtatttt catgcaaata gggggcaaga
     6121 aaactctatg gaaagatggt ggtttaattc gatgttgttt aagaaggagt tcgaacgcag
     6181 gtgtgggcta aataaatcaa tgggcagtct tggtcctatt gaaaatacca atgaagatcc
     6241 aaatcgaaaa gtgaaaaaca ttcatagttg gaggaatcgt gacaattcta gttgcagtaa
     6301 tgttgattat ttattcggcg ttaaagacat tcggaatttc atctctgatg acactttttt
     6361 agttagtgat aggaatggag acagttattc catctatttt gatattgaaa atcatatttt
     6421 tgagattgac aacgatcatt cttttctgag tgaactagaa agttcttttt atagttatcg
     6481 aaactcgaat tatcggaata atggatttag gggcgaagat ccctactata attcttacat
     6541 gtatgatact caatatagtt ggaataatca cattaatagt tgcattgata gttatcttca
     6601 gtctcaaatc tgtatagata cttccattat aagtggtagt gagaattacg gtgacagtta
     6661 catttatagg gccgtttgtg gtggtgaaag tcgaaatagt agtgaaaacg agggttccag
     6721 tagacgaact cgcacgaagg gcagtgattt aactataaga gaaagttcta atgatctcgc
     6781 aggtaactca aaaatacagg catttgtggg ttcaatgcga aaattgttat ggattaaatt
     6841 ataagaaatt tttgaaatca aaaatgaata tttgtgaaca atgtggatat catttgaaaa
     6901 tgagtagttc agatagaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     6961 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7021 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7081 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7141 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7201 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7261 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7321 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7381 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7441 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7501 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7561 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7621 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7681 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7741 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     7801 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     7861 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     7921 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     7981 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8041 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8101 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8161 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8221 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8281 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8341 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8401 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8461 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8521 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8581 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     8641 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     8701 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     8761 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     8821 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     8881 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     8941 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9001 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9061 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     9121 taaaaatagg cgtatcacga ggccctttcg tc
//
