Basic Vector Information
pVMG-TnpR vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pVMG-TnpR vector Sequence
LOCUS 40924_46038 7264 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pVMG-TnpR, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7264)
AUTHORS Oke V, Long SR.
TITLE Bacterial genes induced within the nodule during the
Rhizobium-legume symbiosis
JOURNAL Mol. Microbiol. 32 (4), 837-849 (1999)
PUBMED 10361286
REFERENCE 2 (bases 1 to 7264)
AUTHORS Gao M, Teplitski M.
TITLE RIVET-a tool for in vivo analysis of symbiotically relevant gene
expression in Sinorhizobium meliloti
JOURNAL Mol. Plant Microbe Interact. 21 (2), 162-170 (2008)
PUBMED 18184060
REFERENCE 3 (bases 1 to 7264)
AUTHORS Gao M, Oke V, Teplitski M.
TITLE Direct Submission
JOURNAL Submitted (17-OCT-2007) Soil and Water Science, University of
REFERENCE 4 (bases 1 to 7264)
TITLE Direct Submission
REFERENCE 5 (bases 1 to 7264)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Mol.
Microbiol."; date: "1999"; volume: "32"; issue: "4"; pages:
"837-849"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Mol. Plant
Microbe Interact."; date: "2008"; volume: "21"; issue: "2"; pages:
"162-170"
COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
(17-OCT-2007) Soil and Water Science, University of Florida-IFAS,
1376 Mowry Rd., Cancer "
COMMENT SGRef: number: 4; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7264
/mol_type="other DNA"
/organism="synthetic DNA construct"
regulatory 28..58
/label=Escherichia coli trpA
/note="Escherichia coli trpA"
/regulatory_class="terminator"
misc_feature 103..113
/label=contains stop codons in all three reading frames
/note="contains stop codons in all three reading frames"
regulatory 189..195
/gene="tnpR"
/regulatory_class="ribosome_binding_site"
CDS 205..753
/gene="tnpR"
/label=tnpR
/note="Transposon gamma-delta resolvase from Escherichia
coli (strain K12). Accession#: P03012"
regulatory 756..761
/gene="uidA"
/regulatory_class="ribosome_binding_site"
CDS 788..2596
/label=GUS
/note="beta-glucuronidase"
CDS 3019..3810
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
primer_bind complement(4183..4199)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 4412..4867
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
oriT complement(5206..5315)
/direction=LEFT
/label=oriT
/note="incP origin of transfer"
promoter 5485..5589
/label=AmpR promoter
CDS 5590..6447
/label=AmpR
/note="beta-lactamase"
rep_origin 6621..7209
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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