LOCUS       40924_46038        7264 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pVMG-TnpR, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7264)
  AUTHORS   Oke V, Long SR.
  TITLE     Bacterial genes induced within the nodule during the 
            Rhizobium-legume symbiosis
  JOURNAL   Mol. Microbiol. 32 (4), 837-849 (1999)
  PUBMED    10361286
REFERENCE   2  (bases 1 to 7264)
  AUTHORS   Gao M, Teplitski M.
  TITLE     RIVET-a tool for in vivo analysis of symbiotically relevant gene 
            expression in Sinorhizobium meliloti
  JOURNAL   Mol. Plant Microbe Interact. 21 (2), 162-170 (2008)
  PUBMED    18184060
REFERENCE   3  (bases 1 to 7264)
  AUTHORS   Gao M, Oke V, Teplitski M.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2007) Soil and Water Science, University of 
REFERENCE   4  (bases 1 to 7264)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7264)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. 
            Microbiol."; date: "1999"; volume: "32"; issue: "4"; pages: 
            "837-849"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Mol. Plant 
            Microbe Interact."; date: "2008"; volume: "21"; issue: "2"; pages: 
            "162-170"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (17-OCT-2007) Soil and Water Science, University of Florida-IFAS, 
            1376 Mowry Rd., Cancer "
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7264
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      28..58
                     /label=Escherichia coli trpA
                     /note="Escherichia coli trpA"
                     /regulatory_class="terminator"
     misc_feature    103..113
                     /label=contains stop codons in all three reading frames
                     /note="contains stop codons in all three reading frames"
     regulatory      189..195
                     /gene="tnpR"
                     /regulatory_class="ribosome_binding_site"
     CDS             205..753
                     /gene="tnpR"
                     /label=tnpR
                     /note="Transposon gamma-delta resolvase from Escherichia
                     coli (strain K12). Accession#: P03012"
     regulatory      756..761
                     /gene="uidA"
                     /regulatory_class="ribosome_binding_site"
     CDS             788..2596
                     /label=GUS
                     /note="beta-glucuronidase"
     CDS             3019..3810
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     primer_bind     complement(4183..4199)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4412..4867
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     oriT            complement(5206..5315)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     promoter        5485..5589
                     /label=AmpR promoter
     CDS             5590..6447
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6621..7209
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aagcttgcat gcctgcaggt cgagatcccc agcccgccta atgagcgggc ttttttttgg
       61 tcgacaccgg tcacgtgcct aggactagtg gatccctcga gctgactaac tagctctaga
      121 tgcatgctcg agcggccgcc agtgtgatgg atatctgcag aattcggctt ctagatctca
      181 attgagaatt taggataaat ttttatgcga ctttttggtt acgcacgggt atcaaccagc
      241 cagcaatctc tcgatattca ggttcgggca ctcaaagacg caggcgtgaa agcaaatcgc
      301 atctttactg acaaggcatc gggcagttca tgcgatcgga aagggctgga cttgctgagg
      361 atgaaggtgg aggaaggtga cgtcatcttg gtgaagaaac ttgaccgcct tgggcgcgat
      421 actgctgaca tgatccagtt aataaaagag tttgacgccc aaggtgtatc cattcggttt
      481 attgatgacg gaatcagtac cgatggggag atgggtaaaa tggttgtcac tattctatct
      541 gcagtggccc aggcagaacg acagagaata ctagagcgta ccaatgaagg tcgccaagag
      601 gcaatggcaa aaggagttgt ttttggtaga aaaagaaaaa tagatagaga tgcagtatta
      661 aatatgtggc aacaggggtt aggtgcctca catatatcaa aaacaatgaa tattgctcgt
      721 tcaacagtat ataaagtaat aaatgaaagc aactaaggag gaaagtcgac tctagaggat
      781 ctcgaccatg gtccgtcctg tagaaacccc aacccgtgaa atcaaaaaac tcgacggcct
      841 gtgggcattc agtctggatc gcgaaaactg tggaattgat cagcgttggt gggaaagcgc
      901 gttacaagaa agccgggcaa ttgctgtgcc aggcagtttt aacgatcagt tcgccgatgc
      961 agatattcgt aattatgcgg gcaacgtctg gtatcagcgc gaagtcttta taccgaaagg
     1021 ttgggcaggc cagcgtatcg tgctgcgttt cgatgcggtc actcattacg gcaaagtgtg
     1081 ggtcaataat caggaagtga tggagcatca gggcggctat acgccatttg aagccgatgt
     1141 cacgccgtat gttattgccg ggaaaagtgt acgtatcacc gtttgtgtga acaacgaact
     1201 gaactggcag actatcccgc cgggaatggt gattaccgac gaaaacggca agaaaaagca
     1261 gtcttacttc catgatttct ttaactatgc cggaatccat cgcagcgtaa tgctctacac
     1321 cacgccgaac acctgggtgg acgatatcac cgtggtgacg catgtcgcgc aagactgtaa
     1381 ccacgcgtct gttgactggc aggtggtggc caatggtgat gtcagcgttg aactgcgtga
     1441 tgcggatcaa caggtggttg caactggaca aggcactagc gggactttgc aagtggtgaa
     1501 tccgcacctc tggcaaccgg gtgaaggtta tctctatgaa ctgtgcgtca cagccaaaag
     1561 ccagacagag tgtgatatct acccgcttcg cgtcggcatc cggtcagtgg cagtgaaggg
     1621 ccaacagttc ctgattaacc acaaaccgtt ctactttact ggctttggtc gtcatgaaga
     1681 tgcggactta cgtggcaaag gattcgataa cgtgctgatg gtgcacgacc acgcattaat
     1741 ggactggatt ggggccaact cctaccgtac ctcgcattac ccttacgctg aagagatgct
     1801 cgactgggca gatgaacatg gcatcgtggt gattgatgaa actgctgctg tcggctttaa
     1861 cctctcttta ggcattggtt tcgaagcggg caacaagccg aaagaactgt acagcgaaga
     1921 ggcagtcaac ggggaaactc agcaagcgca cttacaggcg attaaagagc tgatagcgcg
     1981 tgacaaaaac cacccaagcg tggtgatgtg gagtattgcc aacgaaccgg atacccgtcc
     2041 gcaaggtgca cgggaatatt tcgcgccact ggcggaagca acgcgtaaac tcgacccgac
     2101 gcgtccgatc acctgcgtca atgtaatgtt ctgcgacgct cacaccgata ccatcagcga
     2161 tctctttgat gtgctgtgcc tgaaccgtta ttacggatgg tatgtccaaa gcggcgattt
     2221 ggaaacggca gagaaggtac tggaaaaaga acttctggcc tggcaggaga aactgcatca
     2281 gccgattatc atcaccgaat acggcgtgga tacgttagcc gggctgcact caatgtacac
     2341 cgacatgtgg agtgaagagt atcagtgtgc atggctggat atgtatcacc gcgtctttga
     2401 tcgcgtcagc gccgtcgtcg gtgaacaggt atggaatttc gccgattttg cgacctcgca
     2461 aggcatattg cgcgttggcg gtaacaagaa agggatcttc actcgcgacc gcaaaccgaa
     2521 gtcggcggct tttctgctgc aaaaacgctg gactggcatg aacttcggtg aaaaaccgca
     2581 gcagggaggc aaacaatgaa tcaacaactc tcctggcgca ccatcgtcgg ctacagcctc
     2641 ggtggggaat tcgatatcaa gctagcttca cgctgccgca agcactcagg gcgcaagggc
     2701 tgctaaagga agcggaacac gtagaaagcc agtccgcaga aacggtgctg accccggatg
     2761 aatgtcagct actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta
     2821 gcttgcagtg ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa
     2881 ccggaattgc cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg
     2941 atggctttct tgccgccaag gatctgatgg cgcaggggat caagatctga tcaagagaca
     3001 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     3061 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     3121 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     3181 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     3241 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     3301 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     3361 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     3421 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     3481 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     3541 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     3601 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     3661 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     3721 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     3781 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     3841 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     3901 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     3961 tcatgctgga gttcttcgcc caccccgggc tcgatcccct cgcgagttgg ttcagctgct
     4021 gcctgaggct ggacgacctc gcggagttct accggcagtg caaatccgtc ggcatccagg
     4081 aaaccagcag cggctatccg cgcatccatg cccccgaact gcaggagtgg ggaggcacga
     4141 tggccgcttt ggtcgagatc cccgggtacc gagctcgaat tcactggccg tcgttttaca
     4201 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     4261 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     4321 cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     4381 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
     4441 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4501 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4561 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4621 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4681 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4741 aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     4801 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
     4861 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     4921 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     4981 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     5041 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     5101 ataataatgg tttcttagct cgaattctga taggtgggct gcccttcctg gttggcttgg
     5161 tttcatcagc catccgcttg ccctcatctg ttacgccggc ggtagccggc cagcctcgca
     5221 gagcaggatt cccgttgagc accgccaggt gcgaataagg gacagtgaag aaggaacacc
     5281 cgctcgcggg tgggcctact tcacctatcc tgcccggctg acgccgttgg atacaccaag
     5341 gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata
     5401 ccgaaaaaat cgctataatg accccgaagc agggttatgc agcggaaaag aattcgagca
     5461 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     5521 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     5581 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     5641 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     5701 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     5761 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     5821 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     5881 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     5941 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     6001 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     6061 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     6121 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     6181 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     6241 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     6301 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     6361 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     6421 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     6481 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     6541 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     6601 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6661 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6721 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     6781 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6841 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6901 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6961 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     7021 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     7081 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     7141 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     7201 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     7261 gctc
//
