Basic Vector Information
- Vector Name:
- pVLH-1
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7823 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Deitsch KW, Wellems TE.
- Promoter:
- SP6
pVLH-1 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pVLH-1 vector Sequence
LOCUS 40924_46028 7823 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pVLH-1, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7823) AUTHORS Deitsch KW, Wellems TE. TITLE Direct Submission JOURNAL Submitted (08-JAN-1998) LPD/NIAID, NIH, Bldg 4, Rm B1-34, Bethesda, MD 20892, USA REFERENCE 2 (bases 1 to 7823) TITLE Direct Submission REFERENCE 3 (bases 1 to 7823) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted (08-JAN-1998) LPD/NIAID, NIH, Bldg 4, Rm B1-34, Bethesda, MD 20892, USA" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..7823 /mol_type="other DNA" /organism="synthetic DNA construct" source 52..2651 /chromosome="12" /strain="Dd2" /mol_type="genomic DNA" /db_xref="taxon:5833" /organism="Plasmodium falciparum" regulatory 33..42 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" regulatory 52..2651 /gene="var7b" /label=variant surface antigen regulatory region /note="variant surface antigen regulatory region" /regulatory_class="promoter" gene 52..2651 /gene="var7b" /label=var7b CDS 2662..4308 /label=luciferase /note="firefly luciferase" primer_bind complement(4892..4908) /label=SK primer /note="common sequencing primer, one of multiple similar variants" promoter complement(4945..4963) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(4981..4997) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(5005..5021) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(5029..5059) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(5074..5095) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(5383..5971) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(6145..7002) /label=AmpR /note="beta-lactamase" promoter complement(7003..7107) /label=AmpR promoter rep_origin complement(7188..7643) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 7784..7800 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 7807..7823 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase"
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