LOCUS       40924_46028        7823 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pVLH-1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7823)
  AUTHORS   Deitsch KW, Wellems TE.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JAN-1998) LPD/NIAID, NIH, Bldg 4, Rm B1-34, Bethesda, 
            MD 20892, USA
REFERENCE   2  (bases 1 to 7823)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7823)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (08-JAN-1998) LPD/NIAID, NIH, Bldg 4, Rm B1-34, Bethesda, MD 20892, 
            USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          52..2651
                     /chromosome="12"
                     /strain="Dd2"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:5833"
                     /organism="Plasmodium falciparum"
     regulatory      33..42
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      52..2651
                     /gene="var7b"
                     /label=variant surface antigen regulatory region
                     /note="variant surface antigen regulatory region"
                     /regulatory_class="promoter"
     gene            52..2651
                     /gene="var7b"
                     /label=var7b
     CDS             2662..4308
                     /label=luciferase
                     /note="firefly luciferase"
     primer_bind     complement(4892..4908)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(4945..4963)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4981..4997)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5005..5021)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5029..5059)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5074..5095)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5383..5971)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6145..7002)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7003..7107)
                     /label=AmpR promoter
     rep_origin      complement(7188..7643)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     7784..7800
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7807..7823
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggccgcggga ttatggtacc
       61 gtatagacaa accagaccaa attcatttat tatatgattt aagaagattt catccttttt
      121 tataatactg ctttcaaggt ttcatacaaa atcttttccc ttttttttgt acattacgat
      181 agagaaatat atatatatat atatatatat atatatatat atagcataac atatgttacc
      241 atcacatata ttaccataaa tcatatttat attgatgcat tatacatgaa gtttatgtag
      301 attgaaagat aattatagat atataattat ttgtgcattt atatatgtta gataggttta
      361 gtatgtactt gtatctctct atttatatat atattttgag tgtaaccaag cgtatatagt
      421 tataaagatt gttcagtata tgttggtatg tgaatgcatg catatgagtc tatcttattt
      481 ttggtatata tatctatgtt catatagata tgttttaggg atgaggttta tgtttttgtg
      541 tggatatata tatacaaaat acataattaa taatatacca aacataagaa tagtaagtat
      601 acaatatata taaaaataaa ataaaaacat aataatagtt atagttaata taacatcctt
      661 tttttcagtt ttatttatta tttttatttt attttatttt attattattt tttttttttg
      721 ttatctctat attacaaata tatatagaca tattactatt tccagtataa ataaatttgt
      781 tgtacatata tgtatgtata ttatctgatt attatatata tatgtatata ttttatatgt
      841 actgtatttg aattaggtat gcatcctata tgtatattat atacattctc tctagaaata
      901 tgctcaatat atatatgtat cctcaatata tatgtatttt gtcttattaa aggttatatt
      961 ttttttatac atatattaat gtatacttta ataaatagtt taaccatatt agtaatatat
     1021 aagtattgaa atctatatat ttatataatt gtattatatt tgtaaaatgt ttttgataat
     1081 ttgatcaata tcaatgtaga gaaaatgtac attttattaa cacaatacat ttgaatatat
     1141 atatatatat atatatatat atatatatat ataatttttg accttaatgt tttttttata
     1201 ttataaaaac aaaataaagg aaatatataa aatacatgga atacatggaa tacataaaat
     1261 gaagtatata aaatacatga aatatataaa ataaataaaa aaaaagcata atttgttttt
     1321 tattaatata atagtccttt tcttatgtta tttttttttc tacttttttt ttatttcttt
     1381 cttttttttt ttttgatgtt gtacatgtat attatataat acattatata ttataatatt
     1441 ttataaacta tattatataa caacaaaaat taatatttat aatatactac tttcaatatg
     1501 ataaaatact aaaaaaattt atatacatag tatatatgta tttattttca tagatatatg
     1561 gtagataata tagatagaaa ggtaatattt cctttttctc ttctatcata tgagaaataa
     1621 aaatatatat ataattcaat aagaaataga taattgatta tgggattaat ataaatataa
     1681 tatatatata catatatatg ttcttcgatt atagttctca tatttttttt tttttttttt
     1741 tttttttccc ccattctata taatatatat aggattatat aaataaaaat ctacattaaa
     1801 tataatatgt tctctttcat agttttggac aaaatatttt aatttattat gcgatattta
     1861 atcttttaaa aatatatata tatataataa ttatattgat gtgaaatatt atttaaaata
     1921 tatatatatg catatatgtg tgggtatatt ctcattaata taatatgatt atattgtaca
     1981 aaataattat atattatagt gactgaatta aataatatat atatatataa gtgaaaaata
     2041 ttagtatgaa atattataat attatcatgt attattaaaa ttataataat atagttaata
     2101 gtaatgattt ataatattta tattcccaca ttatcatata tttatattat atatatataa
     2161 attaaaatat atatatattc gtcaaatacg tacacatata tatatatatg tataacatgt
     2221 gagtattatt ttatacatca catcgattac attttagcgt tttttttccc cagatcacat
     2281 atagtacgac taagaaacaa aataacatca taacaaacat agtgattatc aatacatgat
     2341 attaccacat aatataaagt attaaataat attattgcat gttagtgata actactatat
     2401 catatacacc actactaact atcactacat agtaacagta gtagtcacaa tcatagcatc
     2461 atggtaatat agattttcat tcatatcttc cttatcgtta gttttccata cagtattaat
     2521 atgtatttat gttataatgg tagactatgt taacaatgta tgaatgacta tcgtaaatta
     2581 ataatagata catgaaaact gtgtatatgt atgtgtgcat ttacaacata atgtagtccg
     2641 gagaatacaa aaatgctgca tgaagacgcc aaaaacataa agaaaggccc ggcgccattc
     2701 tatccgctgg aagatggaac cgctggagag caactgcata aggctatgaa gagatacgcc
     2761 ctggttcctg gaacaattgc ttttacagat gcacatatcg aggtggacat cacttacgct
     2821 gagtacttcg aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca
     2881 aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc
     2941 gcgttattta tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg
     3001 ctcaacagta tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa
     3061 aaaattttga acgtgcaaaa aaagctccca atcatccaaa aaattattat catggattct
     3121 aaaacggatt accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc
     3181 ggttttaatg aatacgattt tgtgccagag tccttcgata gggacaagac aattgcactg
     3241 atcatgaact cctctggatc tactggtctg cctaaaggtg tcgctctgcc tcatagaact
     3301 gcctgcgtga gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat
     3361 actgcgattt taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga
     3421 tatttgatat gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgtttctg
     3481 aggagccttc aggattacaa gattcaaagt gcgctgctgg tgccaaccct attctccttc
     3541 ttcgccaaaa gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct
     3601 ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca
     3661 ggtatcaggc aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag
     3721 ggggatgata aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg
     3781 gatctggata ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt
     3841 cctatgatta tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag
     3901 gatggatggc tacattctgg agacatagct tactgggacg aagacgaaca cttcttcatc
     3961 gttgaccgcc tgaagtctct gattaagtac aaaggctatc aggtggctcc cgctgaattg
     4021 gaatccatct tgctccaaca ccccaacatc ttcgacgcag gtgtcgcagg tcttcccgac
     4081 gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg
     4141 gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga
     4201 ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa
     4261 atcagagaga tcctcataaa ggccaagaag ggcggaaaga tcgccgtgta agcttattta
     4321 ataatagatt aaaaatatta taaaaataaa aacataaaca cagaaattac aaaaaaaata
     4381 catatgaatt ttttttttgt aatcttcctt ataaatatag aataatgaat catataaaac
     4441 atatcattat tcatttattt acatttaaaa ttattgtttc agtatcttta atttattatg
     4501 tatatataaa aataacttac aattttatta ataaacaata tatgtttatt aattcatgtt
     4561 ttgtaattta tgggatagcg atttttttta ctgtctgtat ttttcttttt taattatgtt
     4621 ttaattgtat tttattttta ttattgttct ttttatagta ttattttaaa acaaaatgta
     4681 ttttctaaga acttataata ataataaata taaattttaa taaaaattat atttatcttt
     4741 tacaatatga acataaagta caacattaat atatagcttt taatattttt attcctaatc
     4801 atgtaaatct taaatttttc tttttaaaca tatgttaaat atttatttct cattatatat
     4861 aagaacatat ttattaaact gcagcccggg ggatccacta gttctagagc ggccgccacc
     4921 gcgttggatg catagcttga gtattctata gtgtcaccta aatagcttgg cgtaatcatg
     4981 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     5041 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     5101 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     5161 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     5221 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     5281 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     5341 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     5401 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     5461 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     5521 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     5581 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     5641 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     5701 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     5761 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     5821 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     5881 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     5941 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     6001 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     6061 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     6121 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     6181 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     6241 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     6301 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     6361 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     6421 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     6481 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     6541 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     6601 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     6661 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     6721 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     6781 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     6841 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     6901 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     6961 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     7021 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     7081 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgtatgcgg
     7141 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgaaa ttgtaaacgt
     7201 taatattttg ttaaaattcg cgttaaatat ttgttaaatc agctcatttt ttaaccaata
     7261 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     7321 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     7381 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcacccaaat caagtttttt
     7441 gcggtcgagg tgccgtaaag ctctaaatcg gaaccctaaa gggagccccc gatttagagc
     7501 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     7561 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     7621 taatgcgccg ctacagggcg cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg
     7681 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
     7741 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     7801 gaattgtaat acgactcact ata
//
