Basic Vector Information
pVK-lacIQ-Ptac-MuAB vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pVK-lacIQ-Ptac-MuAB vector Sequence
LOCUS 40924_45998 10173 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pVK-lacIQ-Ptac-MuAB, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10173)
AUTHORS Gorshkova NV, Lobanova JS, Tokmakova IL, Smirnov SV, Akhverdyan VZ,
Krylov AA, Mashko SV.
TITLE Mu-driven transposition of recombinant mini-Mu unit DNA in the
Corynebacterium glutamicum chromosome
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 10173)
AUTHORS Gorshkova NV, Lobanova JS, Tokmakova IL, Smirnov SV, Akhverdyan VZ,
Krylov AA, Mashko SV.
TITLE Direct Submission
JOURNAL Submitted (26-SEP-2017) Ajinomoto-Genetika Research Institute, 1st
Dorozhny pr. 1-1, Moscow 117545, Russian Federation
REFERENCE 3 (bases 1 to 10173)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 10173)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(26-SEP-2017) Ajinomoto-Genetika Research Institute, 1st Dorozhny
pr. 1-1, Moscow 117545, Russian Federation"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..10173
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(404..1858)
/direction=LEFT
/label=ori_pCG1
/note="ori_pCG1"
CDS complement(3000..3530)
/label=GmR
/note="gentamycin acetyltransferase"
promoter complement(3719..3747)
/label=Pc promoter
/note="class 1 integron promoter"
primer_bind 4150..4166
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
CDS complement(4215..5294)
/label=lacI
/note="lac repressor"
promoter complement(5295..5372)
/label=lacIq promoter
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
promoter 5390..5418
/label=tac promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 5424..5444
/label=O lac binding site
/bound_moiety="O lac"
protein_bind 5426..5442
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 5480..7468
/gene="A"
/label=A
/note="DDE-recombinase A from Escherichia phage Mu.
Accession#: P07636"
CDS 7510..8445
/gene="B"
/label=B
/note="ATP-dependent target DNA activator B from
Escherichia phage Mu. Accession#: P03763"
primer_bind complement(8944..8960)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 8968..8984
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8992..9022)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(9037..9058)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(9530..10118)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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