LOCUS       40924_45998       10173 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pVK-lacIQ-Ptac-MuAB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10173)
  AUTHORS   Gorshkova NV, Lobanova JS, Tokmakova IL, Smirnov SV, Akhverdyan VZ, 
            Krylov AA, Mashko SV.
  TITLE     Mu-driven transposition of recombinant mini-Mu unit DNA in the 
            Corynebacterium glutamicum chromosome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10173)
  AUTHORS   Gorshkova NV, Lobanova JS, Tokmakova IL, Smirnov SV, Akhverdyan VZ, 
            Krylov AA, Mashko SV.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2017) Ajinomoto-Genetika Research Institute, 1st 
            Dorozhny pr. 1-1, Moscow 117545, Russian Federation
REFERENCE   3  (bases 1 to 10173)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10173)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-SEP-2017) Ajinomoto-Genetika Research Institute, 1st Dorozhny 
            pr. 1-1, Moscow 117545, Russian Federation"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10173
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(404..1858)
                     /direction=LEFT
                     /label=ori_pCG1
                     /note="ori_pCG1"
     CDS             complement(3000..3530)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(3719..3747)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     primer_bind     4150..4166
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(4215..5294)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(5295..5372)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        5390..5418
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    5424..5444
                     /label=O lac binding site
                     /bound_moiety="O lac"
     protein_bind    5426..5442
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             5480..7468
                     /gene="A"
                     /label=A
                     /note="DDE-recombinase A from Escherichia phage Mu.
                     Accession#: P07636"
     CDS             7510..8445
                     /gene="B"
                     /label=B
                     /note="ATP-dependent target DNA activator B from
                     Escherichia phage Mu. Accession#: P03763"
     primer_bind     complement(8944..8960)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8968..8984
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8992..9022)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(9037..9058)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9530..10118)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaggtctgcc tcgtgaagaa ggtgttgctg actcatacca ggcctgaatc gccccatcat
       61 ccagccagaa agtgagggag ccacggttga tgagagcttt gttgtaggtg gacggggatc
      121 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      181 agattatcaa aaaggatcat gcgagcaacc tccataagat cagctaggcg atctttggga
      241 gcagtccttg tcgcgttacg aggtgagccg gtggggaacc gttagctgcc tatggtgtga
      301 gcccccctag agagcttcaa gagcaatcag cccgacctag aaaggaggcc aagagagaga
      361 cctacggggg gaaccgtttt ctgcctacga gatgggcaca ttactgggaa gctttacggc
      421 gtcctcgtgg aagttcaatg cccgcagact taagtgctct attcacggtc tgacgtgaca
      481 cgctaaattc agacatagct tcattgattg tcggccacga gccagtctct ccctcaacag
      541 tcataaacca acctgcaatg gtcaagcgat ttcctttagc tttcctagct tgtcgttgac
      601 tggacttagc tagtttttct cgctgtgctc gggcgtactc actgtttggg tctttccagc
      661 gttctgcggc ctttttaccg ccacgtcttc ccatagtggc cagagctttt cgccctcggc
      721 tgctctgcgt ctctgtctga cgagcaggga cgactggctg gcctttagcg acgtagccgc
      781 gcacacgtcg cgccatcgtc tggcggtcac gcatcggcgg cagatcaggc tcacggccgt
      841 ctgctccgac cgcctgagcg acggtgtagg cacgctcgta ggcgtcgatg atcttggtgt
      901 cttttaggcg ctcaccagcc gcttttaact ggtatcccac agtcaaagcg tggcgaaaag
      961 ccgtctcatc acgggcggca cgccctggag cagtccagag gacacggacg ccgtcgatca
     1021 gctctccaga cgcttcagcg gcgctcggca ggcttgcttc aagcgtggca agtgcttttg
     1081 cttccgcagt ggcttttctt gccgcttcga tacgtgcccg tccgctagaa aactcctgct
     1141 catagcgttt tttaggtttt tctgtgcctg agatcatgcg agcaacctcc ataagatcag
     1201 ctaggcgatc cacgcgattg tgctgggcat gccagcggta cgcggtggga tcgtcggaga
     1261 cgtgcagtgg ccaccggctc agcctatgtg aaaaagcctg gtcagcgccg aaaacgcggg
     1321 tcatttcctc ggtcgttgca gccagcaggc gcatattcgg gctgctcatg cctgctgcgg
     1381 catacaccgg atcaatgagc cagatgagct ggcatttccc gctcagtgga ttcacgccga
     1441 tccaagctgg cgctttttcc aggcgtgccc agcgctccaa aatcgcgtag acctcggggt
     1501 ttacgtgctc gattttcccg ccggcctggt ggctcggcac atcaatgtcc aggacaagca
     1561 cggctgcgtg ctgcgcgtgc gtcagagcaa catactggca ccgggcaagc gattttgaac
     1621 caactcggta taacttcggc tgtgtttctc ccgtgtccgg gtctttgatc caagcgctgg
     1681 cgaagtcgcg ggtcttgctg ccctggaaat tttctctgcc caggtgagcg aggaattcgc
     1741 ggcggtcttc gctcgtccag ccacgtgatc gcagcgcgag ctcgggatgg gtgtcgaaca
     1801 gatcagcgga aaatttccag gccggtgtgt caatgtctcg tgaatccgct agagtcattt
     1861 ttgagcgctt tctcccaggt ttggactggg ggttagccga cgccctgtga gttaccgctc
     1921 acggggcgtt caacattttt caggtattcg tgcagcttat cgcttcttgc cgcctgtgcg
     1981 ctttttcgac gcgcgacgct gctgccgatt cggtgcaggt ggtggcggcg ctgacacgtc
     2041 ctgggcggcc acggccacac gaaacgcggc atttacgatg tttgtcatgc ctgcgggcac
     2101 cgcgccacga tcgcggataa ttctcgctgc cgcttccagc tctgtgacga ccatggccaa
     2161 aatttcgctc gggggacgca cttccagcgc catttgcgac ctagccgcct ccagctcctc
     2221 ggcgtggcgt ttgttggcgc gctcgcggct ggctgcggca cgacacgcat ctgagcaata
     2281 ttttgcgcgc cgtcctcgcg ggtcaggccg gggaggaatc aggccaccgc agtaggcgca
     2341 actgattcga tcctccacta ctgtgcgtcc tcctggcgct gccgagcacg cagctcgtca
     2401 gccagctcct caagatccgc cacgagagtt tctaggtcgc tcgcggcact ggcccagtct
     2461 cgtgatgctg gcgcgtccgt cgtatcgaga gctcggaaaa atccgatcac cgtttttaaa
     2521 tcgacggcag catcgagcgc gtcggactcc agcgcgacat cagagagatc catagctgat
     2581 gattcgggcc aattttggta cttcgtcgtg aaggtcatga caccattata acgaacgttc
     2641 gttaaagttt ttggcggaaa atcacgcggc acgaaaattt tcacgaagcg ggactttgcg
     2701 cagctcaggg gtgctaaaaa ttttgtatcg cacttgattt ttccgaaaga cagattatct
     2761 gcaaacggtg tgtcgtattt ctggcttggt ttttaaaaaa tctggaatcg aaaatttgcg
     2821 gggcgaccga gaagtttttt acagctagca gcgggtgatg ggactaacgc ctgagcctag
     2881 cagcgggtga tgggactaac gcctgagtta agccggagcg ctttgcggcc gcggcgttgt
     2941 gacaatttac cgaacaactc cgcggccggg aagccgatct cggcttgaac gaattgttag
     3001 gtggcggtac ttgggtcgat atcaaagtgc atcacttctt cccgtatgcc caactttgta
     3061 tagagagcca ctgcgggatc gtcaccgtaa tctgcttgca cgtagatcac ataagcacca
     3121 agcgcgttgg cctcatgctt gaggagattg atgagcgcgg tggcaatgcc ctgcctccgg
     3181 tgctcgccgg agactgcgag atcatagata tagatctcac tacgcggctg ctcaaacctg
     3241 ggcagaacgt aagccgcgag agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg
     3301 atgaatgtct tactacggag caagttcccg aggtaatcgg agtccggctg atgttgggag
     3361 taggtggcta cgtctccgaa ctcacgaccg aaaagatcaa gagcagcccg catggatttg
     3421 acttggtcag ggccgagcct acatgtgcga atgatgccca tacttgagcc acctaacttt
     3481 gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tgcgtaacat cgttgctgct
     3541 ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat
     3601 agactgtaca aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc
     3661 gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag
     3721 tttacgaacc gaacaggctt atgtcaactg ggttcgtgcc ttcatccgtt tccacggtgt
     3781 gcgtcacccg gcaaccttgg gcagcagcga agtcgaggca tttctgtcct ggctggcgaa
     3841 acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgcacaga gagctagata
     3901 atagctgctg atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg
     3961 gcagagcatt acgctgactt gacgggacgg cggctttgtt gaataaatcg cattcgccat
     4021 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     4081 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
     4141 cacgacgttg taaaacgacg gccagtgaat tcgagctcgg taccagctaa ctcacattaa
     4201 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     4261 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt ggtttttctt
     4321 ttcaccagtg agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc
     4381 agcaagcggt ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttaac
     4441 ggcgggatat aacatgagct gtcttcggta tcgtcgtatc ccactaccga gatatccgca
     4501 ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg
     4561 gcaaccagca tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa
     4621 ccggacatgg cactccagtc gccttcccgt tccgctatcg gctgaatttg attgcgagtg
     4681 agatatttat gccagccagc cagacgcaga cgcgccgaga cagaacttaa tgggcccgct
     4741 aacagcgcga tttgctggtg acccaatgcg accagatgct ccacgcccag tcgcgtaccg
     4801 tcttcatggg agaaaataat actgttgatg ggtgtctggt cagagacatc aagaaataac
     4861 gccggaacat tagtgcaggc agcttccaca gcaatggcat cctggtcatc cagcggatag
     4921 ttaatgatca gcccactgac gcgttgcgcg agaagattgt gcaccgccgc tttacaggct
     4981 tcgacgccgc ttcgttctac catcgacacc accacgctgg cacccagttg atcggcgcga
     5041 gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg
     5101 ccaatcagca acgactgttt gcccgccagt tgttgtgcca cgcggttggg aatgtaattc
     5161 agctccgcca tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg gctggcctgg
     5221 ttcaccacgc gggaaacggt ctgataagag acaccggcat actctgcgac atcgtataac
     5281 gttactggtt tcacattcac caccctgaat tgactctctt ccgggcgcta tcatgccata
     5341 ccgcgaaagg ttttgcacca ttcgatggtg tatattctga attgagctgt tgacaattaa
     5401 tcatcggctc gtataatgtg tggaattgtg agcggataac aatttcactc tagagatccc
     5461 catcaagcag gtgaataaca tggaactttg ggtatcaccg aaagagtgtg cgaatcttcc
     5521 tggtttgccg aaaacatcgg ctggtgtgat ttatgttgct aaaaagcaag gatggcaaaa
     5581 ccgcactaga gcaggtgtca aaggtggtaa agcaattgaa tacaatgcga actctttacc
     5641 tgttgaagcg aaagcggcgt tattgctgag acaaggagag attgaaacaa gcctggggta
     5701 ttttgaaatc gcccgcccca cgctggaagc ccatgattat gatcgtgagg cactgtggag
     5761 caaatgggat aacgccagcg attcccagcg cagacttgct gaaaaatggt tgcctgcggt
     5821 tcaggctgca gacgaaatgc tgaaccaggg gatttcaacg aaaacggctt ttgcgaccgt
     5881 tgcagggcat taccaggtca gcgcatccac tttgcgggac aagtattacc aggtacagaa
     5941 gtttgcgaag cctgactggg cggctgcact tgttgatgga cgtggagcat cccgtcgcaa
     6001 tgttcacaaa agtgaatttg acgaggatgc ctggcagttt ctgattgcag attatctgcg
     6061 accggaaaaa cccgctttcc gcaaatgtta tgagcgtctg gaactggcag cccgcgagca
     6121 tggctggagt attccctccc gtgccacggc ctttcgccgg attcagcaac tggacgaggc
     6181 aatggttgtt gcctgtcgtg aaggtgaaca tgcactgatg catctgatac cggcacagca
     6241 gcgaactgtg gaacacctgg acgccatgca gtggatcaac ggcgacggtt atctgcataa
     6301 cgtctttgta cgctggttta acggtgatgt gatccgtccg aaaacatggt tctggcagga
     6361 tgtgaaaacc cgaaaaattc tgggctggcg ctgcgatgtg agcgagaaca ttgattcaat
     6421 tcgcctctcg ttcatggatg ttgtgactcg ctacggtatc ccggaggatt ttcacatcac
     6481 cattgataac acccgtggtg ctgcgaataa atggctgacg ggaggcgcgc ccaatcgcta
     6541 ccgctttaag gtaaaagagg acgatccaaa aggactgttt ttactgatgg gggcgaaaat
     6601 gcactggaca agcgttgttg ccggtaaagg ctggggccag gcaaaacctg ttgaacgtgc
     6661 tttcggtgtt ggtgggcttg aggaatacgt tgataagcat ccggcactgg ctggcgcata
     6721 tacggggcca aatccgcagg caaaacctga taactatggc gaccgcgctg ttgatgcaga
     6781 gctgtttctg aaaacccttg ccgaaggtgt ggcgatgttc aatgccagaa caggccgtga
     6841 aacagaaatg tgcgggggca aactctcgtt tgatgatgtt ttcgagcgtg aatacgccag
     6901 aacgattgtg cgtaagccaa ccgaagaaca aaaacggatg ctgttactgc ctgccgaggc
     6961 ggtgaacgtt tcacgcaaag gcgagtttac gcttaaagtt ggcggctccc ttaaaggcgc
     7021 gaaaaacgtt tattacaaca tggcattaat gaatgccggc gtgaaaaaag ttgtggtcag
     7081 gtttgatccg cagcagctac acagcacggt ttattgctac accctggacg gtcggtttat
     7141 ctgtgaagcg gaatgtctgg cacctgttgc atttaatgat gctgcggcag gccgtgaata
     7201 tcgccgccgc cagaaacaac tgaaatctgc gacgaaagca gccattaagg cgcagaaaca
     7261 aatggacgcg ctggaagttg ctgaactgct gccgcagata gccgaaccag cagcaccaga
     7321 atcacgaatt gttggtattt tccggccttc cggtaatacg gaacgggtga agaatcagga
     7381 gcgtgatgat gaatacgaaa ctgagcgtga tgaatatctg aatcattcgc tggatattct
     7441 ggaacagaac agacgtaaaa aagccattta attaacgttt aaacaaaatt taattacgag
     7501 gttattcaga tgaatatttc cgatattcgc gcaggactgc gcacgcttgt agaaaatgaa
     7561 gaaaccacct ttaaacaaat tgctcttgag agcgggcttt ctaccggaac tatcagtagt
     7621 tttatcaatg ataagtacaa cggggataac gagcgtgttt cacaaatgct gcaacgctgg
     7681 ctggaaaaat atcatgcagt ggcagaacta cctgaaccgc cccgctttgt ggaaacgcag
     7741 acggtaaaac aaatctggac aagtatgcgt tttgccagcc tgactgaaag tattgctgtt
     7801 gtatgtggca atcctggtgt gggcaaaacc gaagcggccc gtgaatatcg ccgcaccaat
     7861 aacaatgtct ggatgatcac cattacgcca tcctgtgcca gcgttctgga atgtcttact
     7921 gaactggcgt ttgagctggg aatgaatgac gcaccacgcc gtaaagggcc gctctcccgc
     7981 gccctgcgac gtcgccttga aggtacacag gggctggtta tcatcgacga agctgatcat
     8041 cttggtgccg aagttctgga agaactccgc ctgttacagg aatcaacccg tattggcctt
     8101 gtgctgatgg gaaatcaccg ggtttattca aatatgacgg ggggtaacag aacggttgaa
     8161 tttgcccgtc tgttttcccg tattgcaaag cgcactgcaa ttaataaaac caaaaaagcc
     8221 gatgtaaaag ctattgcgga tgcctggcag attaacggcg aaaaagaact ggagttatta
     8281 cagcagattg cgcagaaacc aggtgcgctt cgcattctga atcattcact tcgccttgca
     8341 gccatgacgg ctcacggtaa aggtgagcgt gttaacgaag attatctgcg tcaggctttc
     8401 cgtgaattag acctggacgt tgatatttca acgctgctgc gtaattaaga aggagaagaa
     8461 attatgatgg cccgaaatat aaaaatggca acggatgcgc agaactggtt acaggctgac
     8521 tcttatacac aagtagcgtc ctgaacggaa cctttcccgt tttccaggat ctgatcttcc
     8581 atgtgacctc ctaacatggt aacgttcatg ataacttctg ctcttcatcg tgcggccgca
     8641 actagccggt tacgcatggg cagcatgatg agcaacgtga ttgcggtctg tgaccgcgaa
     8701 gccgatattc atgcttatct gcaacgacaa actggcgcat aacgagcaat tcgaggtgcc
     8761 tgttgagctt cttgtagttg ctgttctagc tgtgccttgg ttgccatgct ttaagactct
     8821 agtagctttc ctgcgatatg tcatgcgcat gcgtagcaaa cattgtcctg caactcattc
     8881 attatgtgca gtgctcctgt tactagagtc gacctgcagg catgcaagct tggcgtaatc
     8941 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     9001 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     9061 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     9121 aatcggccaa cgcgcgggga gaggcggttt gcgtattggc gaacttttgc tgagttgaag
     9181 gatcagatca cgcatcttcc cgacaacgca gaccgttccg tggcaaagca aaagttcaaa
     9241 atcagtaacc gtcagtgccg ataagttcaa agttaaacct ggtgttgata ccaacattga
     9301 aacgctgatc gaaaacgcgc tgaaaaacgc tgctgaatgt gcgagcttct tccgcttcct
     9361 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     9421 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     9481 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     9541 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     9601 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     9661 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     9721 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     9781 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     9841 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     9901 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     9961 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    10021 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    10081 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    10141 cggggtctga cgctcagtgg aacgatccgt cga
//
