Basic Vector Information
- Vector Name:
- pVISIA11
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7327 bp
- Type:
- Gastrointestinal evaluation vector
- Replication origin:
- ori
- Source/Author:
- Campos J, Mongodin EF, Fraser CM, Khan S.
- Promoter:
- tet
pVISIA11 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pVISIA11 vector Sequence
LOCUS 40924_45978 7327 bp DNA circular SYN 18-DEC-2018
DEFINITION Gastrointestinal evaluation vector pVISIA11, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7327)
AUTHORS Campos J, Mongodin EF, Fraser CM, Khan S.
TITLE In vivo gastrointestinal evaluation technology: barcoded molecular
tags to identify genetic determinants of E. coli colonization
efficiency in mice
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7327)
AUTHORS Huang S-H., Wu J, Cappiello C, Patel S, Hand N, Sridharan V,
Zeichner SL.
TITLE Direct Submission
JOURNAL Submitted (05-MAR-2015) Center for Cancer and Immunology Research,
Children's National Medical Center, 111 Michigan Ave NW, Washington,
DC 20010, USA
REFERENCE 3 (bases 1 to 7327)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 7327)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(05-MAR-2015) Center for Cancer and Immunology Research, Children's
National Medical Center, 111 Michigan Ave NW, Washington, DC 20010,
USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7327
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 133..161
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
CDS 209..1396
/label=TcR
/note="tetracycline efflux protein"
promoter 1701..1805
/label=AmpR promoter
misc_feature 1809..1886
/note="CTB signal sequence; Cholera toxin B signal
sequence"
CDS 1923..1949
/label=HA
/note="HA (human influenza hemagglutinin) epitope tag"
misc_feature 1989..3332
/label=beta-barrel of the AIDA-I autotransporter
/note="beta-barrel of the AIDA-I autotransporter"
CDS 3722..4513
/gene="thyA"
/label=thyA
/note="Thymidylate synthase from Escherichia coli O139:H28
(strain E24377A / ETEC). Accession#: A7ZQT3"
misc_feature 4705..5364
/label=barcode region
/note="barcode region"
rep_origin complement(5510..6098)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6292..7149)
/label=AmpR
/note="beta-lactamase"
regulatory complement(7150..7327)
/label=bla promoter
/note="bla promoter"
/regulatory_class="promoter"
promoter complement(7150..7241)
/gene="bla"
/label=AmpR promoter
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