LOCUS       V002238                 7327 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002238
VERSION     V002238
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7327)
  AUTHORS   Campos J, Mongodin EF, Fraser CM, Khan S.
  TITLE     In vivo gastrointestinal evaluation technology: barcoded molecular
            tags to identify genetic determinants of E. coli colonization
            efficiency in mice
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7327)
  AUTHORS   Huang S-H., Wu J, Cappiello C, Patel S, Hand N, Sridharan V,
            Zeichner SL.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2015) Center for Cancer and Immunology Research,
            Children's National Medical Center, 111 Michigan Ave NW, Washington,
            DC 20010, USA
REFERENCE   3  (bases 1 to 7327)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7327)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (05-MAR-2015) Center for Cancer and Immunology Research, Children's
            National Medical Center, 111 Michigan Ave NW, Washington, DC 20010,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        133..161
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             209..1396
                     /label="TcR"
                     /note="tetracycline efflux protein"
     promoter        1701..1805
                     /label="AmpR promoter"
     misc_feature    1809..1886
                     /note="CTB signal sequence; Cholera toxin B signal
                     sequence"
     CDS             1923..1949
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     misc_feature    1989..3332
                     /label="beta-barrel of the AIDA-I autotransporter"
                     /note="beta-barrel of the AIDA-I autotransporter"
     CDS             3722..4513
                     /gene="thyA"
                     /label="Thymidylate synthase"
                     /note="Thymidylate synthase from Escherichia coli O139:H28
                     (strain E24377A / ETEC). Accession#: A7ZQT3"
     misc_feature    4705..5364
                     /label="barcode region"
                     /note="barcode region"
     rep_origin      complement(5510..6098)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6292..7149)
                     /label="AmpR"
                     /note="beta-lactamase"
     regulatory      complement(7150..7327)
                     /label="bla promoter"
                     /note="bla promoter"
                     /regulatory_class="promoter"
     promoter        complement(7150..7241)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 actccgcgca catttccccg aaaagtgcca cctccgcggg acgtctaaga aaccattatt
       61 atcatgacat taacctataa aaataggcgt atcacgaggc acgcgtcctt tcgtcctcaa
      121 gaattctcat gtttgacagc ttatcatcga taagctttaa tgcggtagtt tatcacagtt
      181 aaattgctaa cgcagtcagg caccgtgtat gaaatctaac aatgcgctca tcgtcatcct
      241 cggcaccgtc accctggatg ctgtaggcat aggcttggtt atgccggtac tgccgggcct
      301 cttgcgggat atcgtccatt ccgacagcat cgccagtcac tatggcgtgc tgctagcgct
      361 atatgcgttg atgcaatttc tatgcgcacc cgttctcgga gcactgtccg accgctttgg
      421 ccgccgccca gtcctgctcg cttcgctact tggagccact atcgactacg cgatcatggc
      481 gaccacaccc gtcctgtgga tcctctacgc cggacgcatc gtggccggca tcaccggcgc
      541 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
      601 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
      661 gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg
      721 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgacc
      781 gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat
      841 cgtcgccgca cttatgactg tgttctttat catgcaactc gtaggacagg tgccggcagc
      901 gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc
      961 gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac
     1021 caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta
     1081 cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc
     1141 ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga
     1201 ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcattgg
     1261 accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg
     1321 gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag
     1381 ccgggccacc tcgacctgat aatagatcga tacgcgtatg gaagccggcg gcacctcgct
     1441 aacggattca ccactccaag aattggagcc aatcaattct tgcggagaac tgtgaatgcg
     1501 caaaccaacc cttggcagaa catatccatc gcgtccgcca tctccagcag ccgcacgcgg
     1561 cgcatctcgg gcagcgttgg gtcctggcca cgggtgcgca tgatcgtgct cctgtcctcg
     1621 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     1681 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     1741 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     1801 agagtatgat taaattaaaa tttggtgttt tttttacagt tttactatct tcagcatatg
     1861 cacatggaac acctcaaaat attactcacg gtgtgttaac cctgtcttct cgtggtctgg
     1921 actatccata cgatgtaccg gattacgcgc gttatggaag acgagtgtta accatggaga
     1981 tcttcgaact taatcctaca aaagaaagtg caggtaatac tcttaccgtg tcaaattata
     2041 ctgggacacc gggaagtgtt atttctcttg gtggtgtgct tgaaggagat aattcactta
     2101 cggaccgtct ggtggtgaaa ggtaatacct ctggtcaaag tgacatcgtt tatgtcaatg
     2161 aagatggcag tggtggtcag acgagagatg gtattaatat tatttctgta gagggaaatt
     2221 ctgatgcaga attctctctg aagaaccgcg tagttgccgg agcttatgat tacacactgc
     2281 agaaaggaaa cgagagtggg acagataata agggatggta tttaaccagt catcttccca
     2341 catctgatac ccggcaatac agaccggaga acggaagtta tgctaccaat atggcactgg
     2401 ctaactcact gttcctcatg gatttgaatg agcgtaagca attcagggcc atgagtgata
     2461 atacacagcc tgagtctgca tccgtgtgga tgaagatcac tggaggaata agctctggta
     2521 agcttaatga cgggcaaaat aaaacaacaa ccaatcagtt tatcaatcag ctcggggggg
     2581 atatttataa attccatgct gaacaactgg gtgattttac cttagggatt atgggaggat
     2641 acgcgaatgc aaaaggtaaa acgataaatt acacgagcaa caaagctgcc agaaacacac
     2701 tggatggtta ttctgtcggg gtatacggta cgtggtatca gaatggggaa aatgcaacag
     2761 ggctctttgc tgaaacttgg atgcaatata actggtttaa tgcatcagtg aaaggtgacg
     2821 gactggaaga agaaaaatat aatctgaatg gtttaaccgc ttctgcaggt gggggatata
     2881 acctgaatgt gcacacatgg acatcacctg aaggaataac aggtgaattc tggttacagc
     2941 ctcatttgca ggctgtctgg atgggggtta caccggatac acatcaggag gataacggaa
     3001 cggtggtgca gggagcaggg aaaaataata ttcagacaaa agcaggtatt cgtgcatcct
     3061 ggaaggtgaa aagcaccctg gataaggata ccgggcggag gttccgtccg tatatagagg
     3121 caaactggat ccataacact catgaatttg gtgttaaaat gagtgatgac agccagttgt
     3181 tgtcaggtag ccgaaatcag ggagagataa agacaggtat tgaaggggtg attactcaaa
     3241 acttgtcagt gaatggcgga gtcgcatatc aggcaggagg tcacgggagc aatgccatct
     3301 ccggagcact ggggataaaa tacagcttct gataatagta cctgtgaagt gaaaaatggc
     3361 gcacattgcg cgacattttt tttgtctgcc gtttaccgca cagatgcgta aggagaaaat
     3421 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3481 tgcggcgagc ggtatcagct cactcaaaag cttggctgtc tcaggtttgt tcctgattgg
     3541 ttacggcgcg tttcgcatca ttgttgagtt tttccgccag cccgacgcgc agtttaccgg
     3601 tgcctgggtg cagtacatca gcatggggca aattctttcc atcccgatga ttgtcgcggg
     3661 tgtgatcatg atggtctggg catatcgtcg cagcccacag caacacgttt cctgaggaac
     3721 catgaaacag tatttagaac tgatgcaaaa agtgctcgac gaaggcacac agaaaaacga
     3781 ccgtaccgga accggaacgc tttccatttt tggtcatcag atgcgtttta acctgcaaga
     3841 tggattcccg ctggtgacaa ctaaacgttg ccacctgcgt tccatcatcc atgaactgct
     3901 gtggtttctg cagggcgaca ctaacattgc ttatctacac gaaaacaatg tcaccatctg
     3961 ggacgaatgg gccgatgaaa acggcgacct cgggccagtg tatggtaaac agtggcgcgc
     4021 ctggccaacg ccagatggtc gtcatattga ccagatcact acggtactga accagctgaa
     4081 aaacgacccg gattcgcgcc gcattattgt ttcagcgtgg aacgtaggcg aactggataa
     4141 aatggcgctg gcaccgtgcc atgcattctt ccagttctat gtggcagacg gcaaactctc
     4201 ttgccagctt tatcagcgct cctgtgacgt gttcctcggc ctgccgttca acattgccag
     4261 ctacgcatta ttggtgcata tgatggcgca gcagtgcgat ctggaagtgg gtgattttgt
     4321 ctggaccggt ggcgacacgc atctgtacag caaccatatg gatcaaactc atctgcaatt
     4381 aagccgcgaa ccgcgtccgc tgccgaagtt gattatcaaa cgtaaacccg aatccatctt
     4441 cgactaccgt ttcgaagatt ttgagattga aggctacgat ccgcatccgg gcattaaagc
     4501 gccggtggct atctaattac gaaacatcct gccagagccg acgccagtgt gcgtcggttt
     4561 ttttaccctc cgttaaattc ttcgatccgc cttcccgaaa ttttgcaacg tcctgcaacg
     4621 gcgtaaatag tccggaagat gcgccgaaga aatagaaact ggccaagctt cgcccgggca
     4681 ggaaagaaca tgtttaatta acgcagagtt tgatcctggc tcagacgccg agacctgcca
     4741 ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc catcacagac
     4801 ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt
     4861 gcccatagtg aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact
     4921 ggtgaaactc acccagggat tggcgctgac gaaaaacata ttctcaataa accctttagg
     4981 gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg
     5041 ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa
     5101 aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat
     5161 acggaactcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa
     5221 cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg
     5281 gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgcggtct
     5341 caggatactc ctacgggagg cagcggatta attaagagca aaaggccagc cctaggaaaa
     5401 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     5461 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     5521 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     5581 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     5641 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     5701 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     5761 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     5821 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     5881 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtgggct aactacggct
     5941 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6001 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6061 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6121 cggggtctga cgctcagtgg aacgacgcgc gcgtaactca cgttaaggga ttttggtcat
     6181 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     6241 aatctaaagt atatatgagt aaacttgatc tccgcgggac gtcgacagtt accaatgctt
     6301 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     6361 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gcgctgcgat
     6421 gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc agccagccgg
     6481 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     6541 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     6601 cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     6661 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     6721 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     6781 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     6841 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     6901 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     6961 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     7021 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     7081 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     7141 aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     7201 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca gtgttacaac
     7261 caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg gctcataaca
     7321 ccccttg
//
