Basic Vector Information
pVDHS1 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pVDHS1 vector Sequence
LOCUS 40924_45933 8808 bp DNA circular SYN 18-DEC-2018
DEFINITION Expression vector pVDHS1, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8808)
AUTHORS Vafadarnejad E, Amoozegar MA, Karimifard A.
TITLE Construction of an inducible expression vector for gene knock down
in Haloarchaeum Halobacterium salinarum
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 8808)
AUTHORS Vafadarnejad E, Deldar AA, Amoozegar MA, Bazzaz A, Karimifard A.
TITLE Direct Submission
JOURNAL Submitted (17-MAY-2014) Department of Microbiology, University of
Tehran, Qods Ave, Tehran 14155-6455, Iran
REFERENCE 3 (bases 1 to 8808)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 8808)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(17-MAY-2014) Department of Microbiology, University of Tehran, Qods
Ave, Tehran 14155-6455, Iran"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..8808
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(3..458)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 600..616
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 626..644
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
rep_origin 677..4426
CDS 4824..6032
/gene="hmgA"
/label=hmgA
/note="3-hydroxy-3-methylglutaryl-coenzyme A reductase from
Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879
/ NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2). Accession#:
Q59468"
primer_bind complement(6376..6392)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(6620..6638)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(6659..6675)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(6683..6699)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6707..6737)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(6752..6773)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(7061..7649)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7823..8680)
/label=AmpR
/note="beta-lactamase"
promoter complement(8681..8785)
/label=AmpR promoter
This page is informational only.