LOCUS       V002245                 8808 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002245
VERSION     V002245
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8808)
  AUTHORS   Vafadarnejad E, Amoozegar MA, Karimifard A.
  TITLE     Construction of an inducible expression vector for gene knock down
            in Haloarchaeum Halobacterium salinarum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8808)
  AUTHORS   Vafadarnejad E, Deldar AA, Amoozegar MA, Bazzaz A, Karimifard A.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2014) Department of Microbiology, University of
            Tehran, Qods Ave, Tehran 14155-6455, Iran
REFERENCE   3  (bases 1 to 8808)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8808)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (17-MAY-2014) Department of Microbiology, University of Tehran, Qods
            Ave, Tehran 14155-6455, Iran"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        626..644
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      677..4426
     CDS             4824..6032
                     /gene="hmgA"
                     /label="3-hydroxy-3-methylglutaryl-coenzyme A reductase"
                     /note="3-hydroxy-3-methylglutaryl-coenzyme A reductase from
                     Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879
                     / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2). Accession#:
                     Q59468"
     primer_bind     complement(6376..6392)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(6620..6638)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6659..6675)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6683..6699)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6707..6737)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6752..6773)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7061..7649)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7823..8680)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8681..8785)
                     /label="AmpR promoter"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gaggtcaagc ttctttgatt cgagccattt gttcctcgcc ggttaggtgg
      721 gagagttcgc ccgattcgag tagttggttc acctcggggt aactgagttc gtccggctcg
      781 cgctcccgta actcgtcgag cggctcgccc cgttttacat taacctcgac ctcgacctcc
      841 tcgaccgaat acccgcgctg gaggtatgcc tgtctaacac cgctcgcggc aatcccctcg
      901 aaagcgtcct ttgccaaatc ggacggttcg acggtgtcgg tgatcccgag atataggaga
      961 gcgaacgacc acgacaggac gtttagcgta tgctctttcc cgtctaaatc gccgtcctcg
     1021 aaggcttccg ttctaaggtc ctcgtcgagt tccgaaacca ggagcggcat atcatgtagg
     1081 gcttcatgga gccgctcgac tatcgctcgc cggcgttcgt atcctgcctg tttgctatac
     1141 tccttcggtt tttggagata ccggcggtcg gccggtgaca aaatccctct tgccctttcg
     1201 tcaagactca tgctttacca atcacatctt catgtccgta atgctttata cgttgtgccc
     1261 taaagcggga tatatgtctg ccacggacac gcggattccg gtgtcgaagg atgtgcggcg
     1321 cgacctccgg gttctcaagg cgcgggaagg ccgacggtca tacgatgaga caattgcggt
     1381 ggtgcttgac gcatatctct ctgagaaggt ggattaataa actatagtcg tgactagcga
     1441 actagttgaa tcaaacgacg ccgggagcac acgagtctgc caggacttcg ctcctatcgg
     1501 cgtcgaagga tgcttacgcg gacacggcgg gcgggtggcc cgttgtccta actcaaccaa
     1561 tgtggttggg gcttatggga ttatcggatt ttcacccggc gttagtgttc gcacaaaagc
     1621 gacactaaat gtacgatacg gacgatacga cggcacacga accgactgag aagcgaagcc
     1681 ttcgctttgc gactgacctc gaggcggacg tctcgcttac agactggacc gcccttcggg
     1741 ttcatcgagt aatcttcgac gagacagaga gtcaatttgc gcttctcgag gcactacaaa
     1801 cagcggcgga cggtgtcgac atactcgagt ttgaacaccg actgtcgagt tgtgggtttg
     1861 acgtcgatgg gtcagtcgtg aaacttgcac gacgagctgt cgccggcgag agacgggctc
     1921 tgtgggactt gattcggtat gcgcgacggt tgggacatga tgagatggga cagccacaga
     1981 gcgccttaga ggacgctctc gcgttcactc gtctattggg ctatagcaac cctcaacaga
     2041 cgatagcaat cggtctcaat gcggctttca gagagacgcg gcgcgaccag gccgacgaga
     2101 ttatcgagta cgttctaacg ctgtctgaga gcgtcgacgt ggtccttggt gggtcagaga
     2161 tcgaccgagt gtacttgcac aactactacg gcgatattct cccatcgagt gttacgaatc
     2221 cgtgcaacga cacccgtaat gattactcca ttacgacgct cgtcgacgtg gttgggacgg
     2281 acggacttgc tgtcgagata cttcggacgg taggtgagac tgaaagcgga cacatggcct
     2341 acagcgccct acaatcggcg ttcagtcagt acgataggac cacggtctcg cggcgattga
     2401 acgagaagct aatcgagtat ggtctttgtg agaagtacgg cgctcgcgga aacctacgaa
     2461 tcgagattct cgacaaagga gcgttgtacc tcgaagaatt agccaaagag aaagagcgac
     2521 aaaaggccct tcaggactac gttacgaaca ccccaaaacc aaaccacaat acccgtgtaa
     2581 tcacggaccg ccctcgggga gggagggagg acgaccaccg accgaccgcc gaaggtgagg
     2641 cgtcgacgac gccggacgga cgggttcgct tacctcggta tcacggtgtg gactatcttt
     2701 cgtacgcaga gacggtctca gcggcgaaga caccgccgaa gaaaggcgtt agtgtgacaa
     2761 actaccctgt cgaacccaaa tctgaccggg gagaagccgg gtggggctac cttgaggacg
     2821 agaatacgct cgtcgtctct gcagagtacg actctgccat gtctcatgtg gtgatgattg
     2881 cacgagcgtt actcgatcct aagacgttcg accaggtcct taccgaggac cgtctcgcgg
     2941 aacttgatgg actaatcgaa gatgggacgt acgttcgtgg gtctcgaaac ctcgggtggt
     3001 tggctgactc tgtcgactcg gcgggtgagt acgtcgacgt cctcgaggac gcgagagacg
     3061 agctactcga catgacgcgg tcacttgcac atgaggacta cgagtgtgag acgtccgagt
     3121 acctgagtcg aatcacgaaa acggcgatgg gtctcgcggg aaccgcgttt cacgtcctcg
     3181 atttactcga tattgacgtc gtttgggagg ctcgcttacc cgactacaac cgacacccgg
     3241 agcgatacgg ggaggacaat gccgagttgt tagcaacgac gcttgcgaag aacgcgccga
     3301 ttgcggcgac gtacgggaac cacgtagtcc ggcgactgtt gtttgaagac cgagacgaga
     3361 agcgacgtca gtcgttcgac cctgtcgtcg acgcgagcaa tccgtatgcg aacctgattg
     3421 cgtcgatctc ggtggttggt gacttcggga atcgggcgga ccacgtcgcc ggcgtaatag
     3481 aagaccgact gtcgaaccct ggggagattc acgaggacgc gccggagatt gcgctcaaag
     3541 tccctgtggt cgtcgagcat ggtccgagta cggttgcacg agtaacacgg gcaatgtgtc
     3601 gggcgaaagg tctcgacgcg acgagagacg cgatacggct attctctggg tttgcacgga
     3661 caccgtacga cgtagcccac gcaatcgggc gagggttgtc tcaagaagcg acaccgagag
     3721 agattcggag tagcgaagta cggctttcac tcgcgagtct cccgtcgaag cgtctcttag
     3781 aggacgcaac ccccacggtt cgggcgatga ttagtacgtt gctcgcgacg aatctgagcc
     3841 ttagcaagac agagttagcc gagaaagcgg gaatctcgac gcagtcggtt cggaaccact
     3901 taccgacact tgtagcaatg gggttagtcg acgagaccga gggtcagtgc cgactgaacc
     3961 tttcgttcac agacgagcaa gaacgccaca agcggattct tccatcgttc atgctgaccg
     4021 agtcgttcac tcgagacgtc ctctttgaga cgctagaagc gcttgatgag ttgaccgagg
     4081 aggtaatcga gatatgggta caatgcggac ctaacgggct accggacatc gaccgtctcc
     4141 atacgatgta cgactgggta gactgggtac gtcccttact gtcgagtcta accgaagaac
     4201 gagacgagac ggttcagacg ctgtctattg ggccggagat tgcacagcaa cccctcacgg
     4261 aggcaggttc gtgattgaac taacctttgg taatatgttc gtgatatgcg ttgtattcgg
     4321 gtatctcgta atctcgtggt atctctcgag acagtacgtt catgattaga gtagggtcga
     4381 cgaactctga acctatgaat cgcggtcgga caacaacccc ccatgggacg gtatcgataa
     4441 gcttgatgac catcgggagg tgcgtgtggg cgtcgatgaa gccggggagg acgacgcggc
     4501 cgccgcagtc gacgacccgc gtctcgacgc cggcgagcag tcccacgtcg aacgcgcggc
     4561 cgacgcggac gacctcgccg tcgcggacgg cgacggcttc gtgcgtctcg tcgggcgacc
     4621 cgagggtgtg tacctccgcg ttcgtcagta cgaggtccgc tgccgcggtc atacgttcga
     4681 ccccgacgcg ggagggcaaa acggttcggg aggcggcggg gtggccgagc ggcggtcggt
     4741 cgtcgcgggt gacgccggcc gggtcggtgg cggccgttgg aagaaccgga acgctttagg
     4801 gccggcgcgg gaaacgtcgg agcatgacag acgccgcgtc cctcgcagac cgcgttcggg
     4861 agggcgacct ccgcctccac gaactcgaag cgcacgccga cgccgacacc gccgccgagg
     4921 cgcgccgact gctcgtcgaa tcgcagtccg gcgcgtcgct cgacgcggtc gggaactacg
     4981 gcttccccgc ggaggccgcc gagtccgcca tcgagaacat ggtcggctcg atccaggtgc
     5041 cgatgggcgt cgccggcccc gtcagcgtcg acggcggctc cgtcgccggc gagaagtacc
     5101 tccccctcgc gaccaccgag ggcgcgctcc tcgcgtcggt caaccgcggt tgctcggtca
     5161 tcaacagcgc cggcggcgcg accgcccgcg tcctcaagtc cgggatgacc cgcgcgccgg
     5221 tgttccgcgt cgccgacgtt gccgaggccg aggcgctcgt ctcgtggacc cgcgacaact
     5281 tcgcggcgct gaaggaggcc gcggaggaga cgacgaacca cggcgaactc ctcgacgtga
     5341 cgccgtacgt cgtcggcaac tcggtgtacc tgcgattccg ctacgacacc aaggacgcga
     5401 tggggatgaa catggccacc atcgccaccg aggccgtctg cggcgtcgtc gaagccgaga
     5461 cggccgcctc gctcgtcgcc ctctcgggca acctctgttc cgacaagaag cccgccgcca
     5521 tcaacgccgt cgaggggcgc ggccggagcg tcaccgccga cgttcgaatc ccgcgcgagg
     5581 tcgtcgaaga acgcctgcac accacgcccg aagcggtcgc ggaactcaac acacgcaaga
     5641 acctggtcgg ctccgcgaag gccgcgagcc tcgggttcaa cgcccacgtc gccaacgtcg
     5701 tcgccgcgat gttcctcgcc accgggcagg acgaggcgca ggtcgtcgag ggcgcgaacg
     5761 ccatcacgac cgccgaggtg caggacggcg acctctacgt ctcggtctcc atcgcctccc
     5821 tcgaagtcgg caccgtcggc ggcggcacga aactcccgac gcagtccgag ggcctcgata
     5881 tcctcggcgt cagcggcggc ggcgaccccg ccggctccaa cgccgacgcc ctcgccgaat
     5941 gcatcgccgt cggttccctc gcgggcgaac tctcccttct ctccgcgctc gcctcgcggc
     6001 acctctccag cgcccacgcc gaactcggtc ggtaactcgc gggttcattt tcaccgacag
     6061 cagggacggc tggactgctt ttcttcgcgt tgttccggtc tgttcctccg acttcgcacc
     6121 tcgtcgttca ctttcacttt cactccgcac ctgtgggtcg cgtttattcc gtttccgcgg
     6181 aaacgatgaa aatgcgtccg tcggggagca cccgcgggcg tgtccacacc ccatcccctc
     6241 ccatgccact cttcacacgc ggtacatcga attcagggat gtacaccacc gtggctttac
     6301 actgaatttg ttgtaaaact ccgtccatac tccgacaaat ataaataagg agaaatcatc
     6361 cgggccagtt gcacggatcc actagttcta gagagggccg ccgtgacaga ctcacgcaac
     6421 gcggccgacg ctatcaacgc gtgagccggc tcgtgtcgaa cagattcaga ccgcgagagc
     6481 aacaacaaac aaggagcatt tccaagcgac gtacatcgcc cgaggaaccg accacgacgc
     6541 ggatgctgcc cgttctggta tgagcgcccc ttgatcgcgg tcggttcgta atcgcggatc
     6601 tgagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt
     6661 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     6721 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     6781 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     6841 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     6901 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     6961 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     7021 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     7081 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     7141 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7201 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     7261 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7321 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7381 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7441 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7501 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7561 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7621 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7681 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7741 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7801 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7861 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7921 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     7981 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     8041 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     8101 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     8161 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8221 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     8281 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     8341 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     8401 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     8461 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     8521 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     8581 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     8641 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     8701 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     8761 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
