pVCDcDL1 vector (V002249)

Basic Vector Information

      • Vector Name:
      • pVCDcDL1
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 3430 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Gupta A, Onda M, Pastan I, Adhya S, Chaudhary VK.

pVCDcDL1 vector Vector Map

pVCDcDL13430 bp6001200180024003000CAP binding sitelac promoterlac operatorM13 revDMycloxPf1 oriloxP511AmpR promoterAmpRori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pVCDcDL1 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_45913        3430 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pVCDcDL1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Gupta A, Onda M, Pastan I, Adhya S, Chaudhary VK.
  TITLE     High-density functional display of proteins on bacteriophage lambda
  JOURNAL   J. Mol. Biol. 334 (2), 241-254 (2003)
  PUBMED    14607116
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Gupta A, Chaudhary VK.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-DEC-2002) Biochemistry, University of Delhi South 
            Campus, Benito Juarez Road, New Delhi 110021, India
REFERENCE   3  (bases 1 to 3430)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3430)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Mol. 
            Biol."; date: "2003"; volume: "334"; issue: "2"; pages: "241-254"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-DEC-2002) Biochemistry, University of Delhi South Campus, Benito
            Juarez Road, New Delhi 110021, India"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3430
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             265..594
                     /gene="D"
                     /label=D
                     /note="Capsid decoration protein from Escherichia phage
                     lambda. Accession#: P03712"
     CDS             667..696
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     protein_bind    715..748
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      748..1176
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     protein_bind    complement(1194..1227)
                     /label=loxP511
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     promoter        1525..1629
                     /label=AmpR promoter
     CDS             1630..2487
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2661..3249
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

This page is informational only.