Basic Vector Information
pVCDcDL1 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pVCDcDL1 vector Sequence
LOCUS 40924_45913 3430 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pVCDcDL1, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3430) AUTHORS Gupta A, Onda M, Pastan I, Adhya S, Chaudhary VK. TITLE High-density functional display of proteins on bacteriophage lambda JOURNAL J. Mol. Biol. 334 (2), 241-254 (2003) PUBMED 14607116 REFERENCE 2 (bases 1 to 3430) AUTHORS Gupta A, Chaudhary VK. TITLE Direct Submission JOURNAL Submitted (02-DEC-2002) Biochemistry, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021, India REFERENCE 3 (bases 1 to 3430) TITLE Direct Submission REFERENCE 4 (bases 1 to 3430) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J. Mol. Biol."; date: "2003"; volume: "334"; issue: "2"; pages: "241-254" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (02-DEC-2002) Biochemistry, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021, India" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..3430 /mol_type="other DNA" /organism="synthetic DNA construct" protein_bind 107..128 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 143..173 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 181..197 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 205..221 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" CDS 265..594 /gene="D" /label=D /note="Capsid decoration protein from Escherichia phage lambda. Accession#: P03712" CDS 667..696 /label=Myc /note="Myc (human c-Myc proto-oncogene) epitope tag" protein_bind 715..748 /label=loxP /note="Cre-mediated recombination occurs in the 8-bp core sequence (ATGTATGC) (Shaw et al., 2021)." rep_origin 748..1176 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" protein_bind complement(1194..1227) /label=loxP511 /note="Cre-mediated recombination occurs in the 8-bp core sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are compatible with each other, but incompatible with wild-type loxP sites." promoter 1525..1629 /label=AmpR promoter CDS 1630..2487 /label=AmpR /note="beta-lactamase" rep_origin 2661..3249 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
This page is informational only.