LOCUS       V002249                 3430 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002249
VERSION     V002249
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Gupta A, Onda M, Pastan I, Adhya S, Chaudhary VK.
  TITLE     High-density functional display of proteins on bacteriophage lambda
  JOURNAL   J. Mol. Biol. 334 (2), 241-254 (2003)
   PUBMED   14607116
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Gupta A, Chaudhary VK.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-DEC-2002) Biochemistry, University of Delhi South
            Campus, Benito Juarez Road, New Delhi 110021, India
REFERENCE   3  (bases 1 to 3430)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3430)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Mol.
            Biol."; date: "2003"; volume: "334"; issue: "2"; pages: "241-254"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-DEC-2002) Biochemistry, University of Delhi South Campus, Benito
            Juarez Road, New Delhi 110021, India"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3430
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             265..594
                     /gene="D"
                     /label="Capsid decoration protein"
                     /note="Capsid decoration protein from Escherichia phage
                     lambda. Accession#: P03712"
     CDS             667..696
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     protein_bind    715..748
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      748..1176
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     protein_bind    complement(1194..1227)
                     /label="loxP511"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     promoter        1525..1629
                     /label="AmpR promoter"
     CDS             1630..2487
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      2661..3249
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 ggtctagata aggaggaata acatatgact tcgaaagaaa cctttaccca ttaccagccg
      301 cagggcaaca gtgacccggc tcataccgca accgcgcccg gcggattgag tgcgaaagcg
      361 cctgcaatga ccccgctgat gctggacacc tccagccgta agctggttgc gtgggatggc
      421 accaccgacg gtgctgccgt tggcattctt gcggttgctg ctgaccagac cagcaccacg
      481 ctgacgttct acaagtccgg cacgttccgt tatgaggatg tgctctggcc ggaggctgcc
      541 agcgacgaga cgaaaaaacg gaccgcgttt gccggaacgg caatctcaat tgttggcgga
      601 tccggaccgg tagggcccgg tggcagcgga gctagcgcag tggaacgtta ccttaagaac
      661 gcgtctgagc agaagctgat cagcgaggaa gatcttaatt aagtcgacga attcataact
      721 tcgtataatg tatgctatac gaagttatcg cgccctgtag cggcgcatta agcgcggcgg
      781 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt
      841 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
      901 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
      961 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     1021 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact
     1081 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa
     1141 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgataactt
     1201 cgtatagtat acattatacg aagttatgag ctctttacaa ttttatggtg cactctcagt
     1261 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     1321 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     1381 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     1441 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     1501 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     1561 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     1621 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     1681 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     1741 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     1801 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     1861 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     1921 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     1981 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     2041 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     2101 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     2161 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     2221 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     2281 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     2341 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     2401 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     2461 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     2521 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     2581 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     2641 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     2701 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     2761 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     2821 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     2881 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     2941 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3001 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     3061 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3121 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3181 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     3241 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     3301 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     3361 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     3421 gaagcggaag
//
