pVCC vector (V002250)

Basic Vector Information

      • Vector Name:
      • pVCC
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 5155 bp
      • Type:
      • Transformation and cloning vector
      • Source/Author:
      • Vannini A, Agriesti F, Mosca F, Roncarati D, Scarlato V, Danielli A.

pVCC vector Vector Map

pVCC5155 bp6001200180024003000360042004800flanking region for double homologous recombination in Helicobacter pylori G27 vac::aphA-3/luxCDABE acceptor strainChloramphenicol acetyltransferase from Campylobacter coli. Accession#: P22782MCS; multiple cloning site; unique BamHI, KpnI, SacI, and SnaBI cloning sitesSP6 promoterM13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoterM13 fwdT7 promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pVCC vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_45908        5155 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Transformation and cloning vector pVCC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Vannini A, Agriesti F, Mosca F, Roncarati D, Scarlato V, Danielli A.
  TITLE     A convenient and robust in vivo reporter system to monitor gene 
            expression in the human pathogen Helicobacter pylori
  JOURNAL   Appl. Environ. Microbiol. 78 (18), 6524-6533 (2012)
  PUBMED    22773640
REFERENCE   2  (bases 1 to 5155)
  AUTHORS   Danielli A.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-SEP-2010) Department of Biology, University of 
            Bologna, via Selmi, 3, Bologna, BO 40126, Italy
REFERENCE   3  (bases 1 to 5155)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5155)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2012"; volume: "78"; issue: "18"; 
            pages: "6524-6533"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-SEP-2010) Department of Biology, University of Bologna, via 
            Selmi, 3, Bologna, BO 40126, Italy"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     1..564
                     /note="flanking region for double homologous recombination
                     in Helicobacter pylori G27 vac::aphA-3/luxCDABE acceptor 
                     strain"
     CDS             27..542
                     /codon_start=1
                     /gene="cysS"
                     /product="CysS"
                     /label=cysS
                     /protein_id="ADZ38982.1"
                     /translation="YLLGVHYRSVLNFNEEDLLVSKKRLDKIYRLKQRVLGTLGGINPN
                     FKKEILECMQDDLNVSKALSVLESMLSSTNEKLDQNPKNKALKGEILANLKFIEELLGI
                     GFKDPSAYFQLGVSESEKQEIENKIEERKRAKEQKNFLKADSIREELLKQKIALMDTPQ
                     GTIWEKFF"
     gene            27..542
                     /gene="cysS"
                     /label=cysS
                     /note="Helicobacter pylori cysS cistron 5' end"
     CDS             complement(683..1303)
                     /note="Chloramphenicol acetyltransferase from Campylobacter
                     coli. Accession#: P22782"
     misc_feature    1407..1448
                     /note="MCS; multiple cloning site; unique BamHI, KpnI,
                     SacI, and SnaBI cloning sites"
     gene            1440..2467
                     /gene="luxC"
                     /label=luxC
                     /note="Photorhabdus luminescens luxC cistron 5' end"
     regulatory      1440..1445
                     /gene="luxC"
                     /regulatory_class="ribosome_binding_site"
     misc_recomb     1451..2467
                     /note="flanking region for double homologous recombination
                     in Helicobacter pylori G27 vac::aphA-3/luxCDABE acceptor 
                     strain"
     CDS             1451..2467
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC"
                     /label=luxC
                     /protein_id="ADZ38983.1"
                     /translation="MTKKISFIINGQVEIFPESDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MRHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKES"
     promoter        complement(2476..2494)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2512..2528)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2536..2552)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2560..2590)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2605..2626)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2914..3502)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3676..4533)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4534..4638)
                     /label=AmpR promoter
     primer_bind     5112..5128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5135..5153
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"

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