LOCUS       40924_45908        5155 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Transformation and cloning vector pVCC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Vannini A, Agriesti F, Mosca F, Roncarati D, Scarlato V, Danielli A.
  TITLE     A convenient and robust in vivo reporter system to monitor gene 
            expression in the human pathogen Helicobacter pylori
  JOURNAL   Appl. Environ. Microbiol. 78 (18), 6524-6533 (2012)
  PUBMED    22773640
REFERENCE   2  (bases 1 to 5155)
  AUTHORS   Danielli A.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-SEP-2010) Department of Biology, University of 
            Bologna, via Selmi, 3, Bologna, BO 40126, Italy
REFERENCE   3  (bases 1 to 5155)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5155)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2012"; volume: "78"; issue: "18"; 
            pages: "6524-6533"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-SEP-2010) Department of Biology, University of Bologna, via 
            Selmi, 3, Bologna, BO 40126, Italy"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     1..564
                     /note="flanking region for double homologous recombination
                     in Helicobacter pylori G27 vac::aphA-3/luxCDABE acceptor 
                     strain"
     CDS             27..542
                     /codon_start=1
                     /gene="cysS"
                     /product="CysS"
                     /label=cysS
                     /protein_id="ADZ38982.1"
                     /translation="YLLGVHYRSVLNFNEEDLLVSKKRLDKIYRLKQRVLGTLGGINPN
                     FKKEILECMQDDLNVSKALSVLESMLSSTNEKLDQNPKNKALKGEILANLKFIEELLGI
                     GFKDPSAYFQLGVSESEKQEIENKIEERKRAKEQKNFLKADSIREELLKQKIALMDTPQ
                     GTIWEKFF"
     gene            27..542
                     /gene="cysS"
                     /label=cysS
                     /note="Helicobacter pylori cysS cistron 5' end"
     CDS             complement(683..1303)
                     /note="Chloramphenicol acetyltransferase from Campylobacter
                     coli. Accession#: P22782"
     misc_feature    1407..1448
                     /note="MCS; multiple cloning site; unique BamHI, KpnI,
                     SacI, and SnaBI cloning sites"
     gene            1440..2467
                     /gene="luxC"
                     /label=luxC
                     /note="Photorhabdus luminescens luxC cistron 5' end"
     regulatory      1440..1445
                     /gene="luxC"
                     /regulatory_class="ribosome_binding_site"
     misc_recomb     1451..2467
                     /note="flanking region for double homologous recombination
                     in Helicobacter pylori G27 vac::aphA-3/luxCDABE acceptor 
                     strain"
     CDS             1451..2467
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC"
                     /label=luxC
                     /protein_id="ADZ38983.1"
                     /translation="MTKKISFIINGQVEIFPESDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MRHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKES"
     promoter        complement(2476..2494)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2512..2528)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2536..2552)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2560..2590)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2605..2626)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2914..3502)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3676..4533)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4534..4638)
                     /label=AmpR promoter
     primer_bind     5112..5128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5135..5153
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gaattctgat ggcgaatctt gcgcattatt tactaggggt gcattatcgc tctgttttga
       61 atttcaatga agaagacttg ttagtgagta aaaaacgctt ggataaaatc tatcgtttga
      121 aacagcgcgt tttagggact ttgggaggaa taaatccaaa ctttaaaaaa gaaattttag
      181 agtgcatgca agatgattta aacgtttcta aagcgttgag cgttttagaa agcatgcttt
      241 cttctacgaa tgaaaaactg gatcaaaacc ccaaaaacaa ggctttgaag ggcgaaattt
      301 tagcgaattt gaaattcata gaagaactgc ttggtattgg gtttaaagac cctagcgcgt
      361 atttccagtt aggcgtgagc gagagcgaaa aacaagaaat tgaaaacaag atagaagaaa
      421 gaaaacgcgc caaagaacaa aaaaattttt taaaagccga tagcatcaga gaagaacttt
      481 tgaaacaaaa aatcgctttg atggacaccc cacaaggcac gatttgggag aagttttttt
      541 aaccatggga cgtcgacctg aggtaattat aacccgggcc ctatatatgg atctgcagga
      601 cgcactactc tcgacagaga gtatagaagt gcgcccttta gttcctaaag ggtttttatc
      661 agtgcgacaa actgggattt tatttattca gcaagtcttg taattcatcc aaaaaacggc
      721 aaacatgaaa gccgtcacaa acggcatgat gcacttgaat cgataaggga atatagtatt
      781 ttccgccctc ctcataatac ttcccaaacg taaatatcgg cagtagatag tcatatcctt
      841 tttttagatt taagttaaag ccttcaaagc ttgtccacgg tatcatagaa acagggaaag
      901 tgttttccgg aggattaggc tttgcggaca ttcccattcg ttcaccaaaa gcgtctatat
      961 ccttttgata gttctgaaga aactcagtat agtctgctgt aaactcagtc caaatactcg
     1021 aaaaggtttc agtttcctta tgaaaaactg tgtagcaagg cagcatttct gaaaaaacgc
     1081 ctacctgtcc gttttcatct aatgcggtcc tgaactcttc atgtcgattg atgatcgttg
     1141 taactccata taaaagagtt gggtataact tttttccatc ctttttcaac ttagaaatat
     1201 cgagttttac cgtcatacta tatgtgcagg gcgtattgcc aaaatagtgg tcgaaatact
     1261 cttttcgtgt ccaattattt atatcaatct ttgtgaattg catcatttat cctccgtaaa
     1321 ttccgatttg ttgataactc attatatcat aaatctatcc actatatcat aaatctatcc
     1381 acttttcaat ctatatcacg gttgggggat ccccgggtac cgagctcgaa ttcaggcttg
     1441 gaggatacgt atgactaaaa aaatttcatt cattattaac ggccaggttg aaatctttcc
     1501 cgaaagtgat gatttagtgc aatccattaa ttttggtgat aatagtgttt acctgccaat
     1561 attgaatgac tctcatgtaa aaaacattat tgattgtaat ggaaataacg aattacggtt
     1621 gcataacatt gtcaattttc tctatacggt agggcaaaga tggaaaaatg aagaatactc
     1681 aagacgcagg acatacattc gtgacttaaa aaaatatatg ggatattcag aagaaatggc
     1741 taagctagag gccaattgga tatctatgat tttatgttct aaaggcggcc tttatgatgt
     1801 tgtagaaaat gaacttggtt ctcgccatat catggatgaa tggctacctc aggatgaaag
     1861 ttatgttcgg gcttttccga aaggtaaatc tgtacatctg ttggcaggta atgttccatt
     1921 atctgggatc atgtctatat tacgcgcaat tttaactaag aatcagtgta ttataaaaac
     1981 atcgtcaacc gatcctttta ccgctaatgc attagcgtta agttttattg atgtagaccc
     2041 taatcatccg ataacgcgct ctttatctgt tatatattgg ccccaccaag gtgatacatc
     2101 actcgcaaaa gaaattatgc gacatgcgga tgttattgtc gcttggggag ggccagatgc
     2161 gattaattgg gcggtagagc atgcgccatc ttatgctgat gtgattaaat ttggttctaa
     2221 aaagagtctt tgcattatcg ataatcctgt tgatttgacg tccgcagcga caggtgcggc
     2281 tcatgatgtt tgtttttacg atcagcgagc ttgtttttct gcccaaaaca tatattacat
     2341 gggaaatcat tatgaggaat ttaagttagc gttgatagaa aaacttaatc tatatgcgca
     2401 tatattaccg aatgccaaaa aagattttga tgaaaaggcg gcctattctt tagttcaaaa
     2461 agaaagcttg agtattctat agtgtcacct aaatagcttg gcgtaatcat ggtcatagct
     2521 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     2581 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     2641 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     2701 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     2761 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     2821 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     2881 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     2941 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     3001 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     3061 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     3121 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     3181 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     3241 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     3301 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     3361 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     3421 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     3481 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     3541 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     3601 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     3661 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     3721 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     3781 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     3841 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     3901 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     3961 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     4021 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     4081 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     4141 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     4201 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     4261 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     4321 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     4381 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     4441 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     4501 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     4561 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     4621 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     4681 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
     4741 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     4801 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     4861 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     4921 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc
     4981 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     5041 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     5101 gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat agggc
//
