Basic Vector Information
pVAHS01it vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pVAHS01it vector Sequence
LOCUS 40924_45868 7530 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pVAHS01it, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7530)
AUTHORS Vafadarnejad E, Amoozgar MA, Khansha J, Fallahzade R.
TITLE The rad2 gene of haloarchaeum Halobacterium salinarum is functional
in the repair of ultraviolet light induced DNA photoproducts
JOURNAL Microbiol. Res. 173, 44-49 (2015)
PUBMED 25801970
REFERENCE 2 (bases 1 to 7530)
AUTHORS Vafadarnejad E, Amoozegar MA, Danaie Fard M, Khansha J.
TITLE Direct Submission
JOURNAL Submitted (10-OCT-2014) Department of Microbiology, University of
Tehran, Qods Ave., Tehran, Tehran 14155-6455, Iran
REFERENCE 3 (bases 1 to 7530)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 7530)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol.
Res. 173, 44-49 (2015)"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(10-OCT-2014) Department of Microbiology, University of Tehran, Qods
Ave., Tehran, Tehran 14155-6455, Iran"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7530
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(1112..1128)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(1136..1152)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1160..1190)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(1205..1226)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(1514..2102)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2276..3133)
/label=AmpR
/note="beta-lactamase"
promoter complement(3134..3238)
/label=AmpR promoter
primer_bind 3710..3726
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
CDS 4078..5994
/gene="gyrB"
/label=gyrB
/note="DNA gyrase subunit B from Haloferax lucentense
(strain DSM 14919 / JCM 9276 / NCIMB 13854 / Aa 2.2).
Accession#: P21558"
primer_bind complement(6546..6562)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
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