LOCUS       40924_45868        7530 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pVAHS01it, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7530)
  AUTHORS   Vafadarnejad E, Amoozgar MA, Khansha J, Fallahzade R.
  TITLE     The rad2 gene of haloarchaeum Halobacterium salinarum is functional 
            in the repair of ultraviolet light induced DNA photoproducts
  JOURNAL   Microbiol. Res. 173, 44-49 (2015)
  PUBMED    25801970
REFERENCE   2  (bases 1 to 7530)
  AUTHORS   Vafadarnejad E, Amoozegar MA, Danaie Fard M, Khansha J.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2014) Department of Microbiology, University of 
            Tehran, Qods Ave., Tehran, Tehran 14155-6455, Iran
REFERENCE   3  (bases 1 to 7530)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7530)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol. 
            Res. 173, 44-49 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-OCT-2014) Department of Microbiology, University of Tehran, Qods
            Ave., Tehran, Tehran 14155-6455, Iran"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7530
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1112..1128)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1136..1152)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1160..1190)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1205..1226)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1514..2102)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2276..3133)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3134..3238)
                     /label=AmpR promoter
     primer_bind     3710..3726
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4078..5994
                     /gene="gyrB"
                     /label=gyrB
                     /note="DNA gyrase subunit B from Haloferax lucentense
                     (strain DSM 14919 / JCM 9276 / NCIMB 13854 / Aa 2.2). 
                     Accession#: P21558"
     primer_bind     complement(6546..6562)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgggctttga catgccgaac cgcttcacgc cggagtaccg cgagatgctg tacgcgaagg
       61 cgcagagcct cgaacggggc cttcgggagc ggtgggggag ccttctgcac acggggatgg
      121 tgacgctcac ggcgtcgagt acggacgacg agggacggct gcggcctccg ctggaacact
      181 tcgaggacct gctggagtcg tgggaggctg ttaggcgggc gctggcgcga gttttggagg
      241 gtagagagtg ggagtacctc gccatccttg aaccgcacga gagcgggtac gtacacatcc
      301 atcttggcgt gtttgtgagg gggccggtgg tcgccgagca gttcgagccg gtgctggacg
      361 cgcacctgcg aaactgcccg acggcgggcg aggacgccca tcaggtgttc gacgagaacg
      421 gcgacgagga cgcggtgaga gttcggcgct cgtcgcaccc gtcgcgcagc ggtggcgtcg
      481 agaacctcgg ggcgtacctg gcggcgtaca tggccggcga gtacggctca gagccgtctg
      541 agatgcctga gaacgtccgt gcattctacg cgacgatgtg ggcgagtggt cggcagtggt
      601 tccgtccgag caacggcgca caggagctga tgcaaccgga ggaggacgac gagggcgaca
      661 gcatcgagga gtgggagatg gtgggcatcg cgccggaggg ggatctcggg gacatcatcg
      721 aggtggaccc gtcggagcca cggagcgacc cgtacaggag gttgcgaacg ccgccaccgg
      781 gtggttaaga cgctcagacg gcggtctggc cttgcccacg gagtgggttg aagaagtggg
      841 tcgtagagcg gacgcgatga gagcgatgta ggtgagctac agcagattgg gatgtcgatg
      901 agtgtcgacc taccagattt cggatattct aagagtgtgt gatttcgatg ggctgtagct
      961 acctgaactg gggtcgcgtg tgtaggggtt gacgtagctg ggatttgacg gtcaattgcc
     1021 gactaatatt tgataagcta tagccggcta atcgtagcag gtactgattt ctgacatacc
     1081 ggaccaataa tggaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     1141 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     1201 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     1261 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     1321 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     1381 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     1441 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     1501 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     1561 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1621 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1681 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1741 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1801 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1861 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1921 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     1981 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     2041 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     2101 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     2161 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     2221 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     2281 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     2341 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     2401 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     2461 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     2521 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     2581 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     2641 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     2701 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     2761 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     2821 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     2881 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     2941 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     3001 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     3061 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     3121 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     3181 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     3241 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     3301 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     3361 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     3421 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     3481 atgcggcatc agagcagatt gtactgagag tgcaccatgc ggtgtgaaat accgcacaga
     3541 tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg
     3601 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     3661 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     3721 gccagtgaat tcgagctcgg tacccggggg atctcccgct ggcccttcgc cagctcctct
     3781 gcgatccccg gctcgtcacc gagtgtcgac tggaacgagg tcatttcctg atgcgcatct
     3841 acccagtccc gcgttaagta ctcaccggca gcggagtgaa agtgaactcg gggcgttgac
     3901 gcgcttcgag gggcgttcgc gccggaacgc cgaatcatga tacacagagt cacccacgaa
     3961 acgcgggtcg gtcggtcggc gtctccgatg tcggaaaccg ctacgcggga ctccagtaag
     4021 tagtacgcgc ccgcgcgtgc gagcctttaa gggacgggct atcctaggcc tgttcagatg
     4081 tctcaggata acgagtacgg ggccggacag attcaggtcc tcgaagggct tgaagccgtt
     4141 cgaaagcgtc cggcgatgta catcgggtcc accgattctc gcggactcca ccacctcgtc
     4201 tacgaagtcg tcgacaactc catcgacgag gcgctcgcgg gccactgcga cgccatcgag
     4261 gtcgccctcc acgaggacgg ctctgtcagc gtcaccgaca acggccgcgg gattccggta
     4321 ggtacgcacg aacagtacga ccgacccgcg ctggaggtca tcatgaccgt cctccacgcc
     4381 ggcgggaagt tcgacaacaa gtcctaccaa gtctccggcg gcctccacgg cgtcggcgtc
     4441 accgtcgtca acgcgctgtc gagcgaactc gaagtcgagg tcaaacacga cggcgcggtg
     4501 tggacacacc gcttcgaggt cggcgagccg caggtcgagg agttcgagcg cgtgcgcgac
     4561 ctcgaacccg gcgaagacac cggaacgacc atccgctttt ggccggacga cggtatcttc
     4621 gagacgaccg agttcgactt caagacgctc gaaaaccgcc tgcgagagct cgcgttcctc
     4681 aactccgggg tcgagatttc gctctccgac gagcggaccg acgagtcgag cacgttcctc
     4741 ttcgagggcg gcatccgcga gttcgtcgag tacctcaacg agaccaagac ggcgctccac
     4801 gacgacgtca tctactacga cgacgagtcg gagggcatcg aggtcgaaat cgccatgcag
     4861 gcgaccgacg agctccaggg gtcgattcac gccttcgcca acaacatcaa cacccgcgag
     4921 ggcggcacgc atctgaccgg gttcaagacg gcgctcaccc gcgtcgtcaa cgactacgcg
     4981 aacagccacg acatgctgga cgacctcgac ggcgacaacc tccgcggcga ggacgttcgc
     5041 gagggactca ccgccgtcat ctccgtcaag caccccgacc cgcagttcga gggccagacg
     5101 aagaccaagc tcgggaattc cgaggtccgc ggcatcgtcg agagcgtcac ccaccaacag
     5161 ctcgggacgt tcttcgagga gaacccggac acggcgacgg ccatcatctc gaaggccgtc
     5221 gaggccgccc gcgcccgcaa ggccgcgaag caggccgagg agctcacccg ccgcaagtcc
     5281 gcgctcgaat ctacctcgct gccggggaag ctcgcggact gccagagccg cgacccctcg
     5341 gagtccgaac tgttcatcgt ggagggcgac tccgcgggcg ggtcggccaa gcagggccgc
     5401 gaccgcaagt tccaggcgat tttgcccctc aaggggaaga ttctgaacgt cgagaaacac
     5461 cgcctcgacc gcattctcga aaacgacgag atacgggcgc tcatcaccgc cattggcggc
     5521 ggcgtcggcg acgagttcga catcgagaag gcgcgctacc agcggctcat cctgatgacc
     5581 gacgccgacg tcgacggcgc gcacatccgg acgctgctgc ttacgcttct gtaccggcac
     5641 atgcgcccgc tcatcgaggc cggctacgtg tacgcggctc aaccgccgct gtaccgcgtc
     5701 cgctaccgcg gcaacaccta cgacgcgatg gacgaggccg agcgggaccg catcatcgag
     5761 gaggaatgca acggcaaccc cacgcaggtc cagcggttca agggactcgg tgagatgaac
     5821 cccgaccagc tgtgggacac gacgatgaac cccgaaaacc gcgtcctcaa gcgcatcacc
     5881 gtcgaggacg ccgccgccgc cgaccggatg ttcaacatcc tgatgggcga cgccgtcggg
     5941 ccgcgaaagc agttcatcaa ggaccacgcg aacgacgccg aatgggtaga catctaatat
     6001 gagttctgac gcaccagatt cgttcgaacc gggcgcgggt atcgcggccg aagtcaagaa
     6061 cgcgcgcatc gaagacgaga tggagcagtc gtacatcgac tacgcgatgt cggtcatcgc
     6121 ggggcgggcg ctgcccgacg tacgcgacgg cctgaagccg gtccaccggc gcatcctgta
     6181 tgcgatgcat accgacgcga aagccgacgc ggacgacgag taacggctcc ccgcgagact
     6241 tttttcgcgg actcccgccg gagagccgcg gctccgaccg tcgctccccg aagacattta
     6301 tctcggccgg aggtcctacc ggtatgagcg tgcgaccgcg gtcgagaatc gtcggcgtct
     6361 cggcgctcgc gttcgtcgtc gtcctcggcg gcctgaccgc gcttcgggcg tggaacctcg
     6421 aaccgggatc ttggcgtaga attcagggat gtacaccacc gtggctttac actgaatttg
     6481 ttgtaaaact ccgtccatac tccgacaaat ataaataagg agaaatcatc cgggccagtt
     6541 gcacggatcc actagttcta gagagggccg ccgtgacaga ctcacgcaac gcggccgacg
     6601 ctatcaacgc gtgagccggc tcgtgtcgaa cagattcaga ccgcgagagc aacaacaaac
     6661 aaggagcatt tccaagcgac gtacatcgcc cgaggaaccg accacgacgc ggatgctgcc
     6721 cgttctggta tgagcgcccc ttgatcgcgg tcggttcgta atcgcggatc tgagctcaac
     6781 tgtggccgcg cggtattttc tccgaatcgg ggtttttttc gtatgtagct ggcagcatgc
     6841 ggaccaatac tggctccacg ccgcaactaa taactggata gctgaatgac ggtggagtaa
     6901 cagctacgca acgtgaaagg tgccccggag aagggccggg acaaaaccgg aaggggccgt
     6961 acccgcggcc tgcaaccggc aaacgaacca ccttgcctgg ctggttcact cggcccttct
     7021 tcgtccgtcc gcttaggtct ttcccgacgg cgagacggct ggtatatgtg ccccaggtcc
     7081 ctctcagggg ccgcaacagc cggcaggcgg gggatctccc gtgctgtcca gcatccccgc
     7141 agtgaaggga tgggtgaaag cccggaacgg gccgcgcagc ggcccgcagc ccggaacaca
     7201 ggccgcaacg cggccgtcct cgttcccgga acggaggggt tccggtgagc acacctaatc
     7261 agccgggtga accgcccgac cggcctgcca actcgggcgg ttctcggcaa cctttgaata
     7321 accgtgcagg cccggagaac gacagcctca gcgcctcaca cgtgccgatg gcgaaacgag
     7381 acgggatgaa actgcgggat gaactgacct tcgacacgag ccgggccgtc aaggcggtgt
     7441 cgtgggggga ggccatcgac cgcttccaga gctggtacga cgaccagcgt ggaacgcaga
     7501 tcgtcgtcga gaacgagctg ggcgagacgg
//
