pUS23 vector (V002273)

MSDS

Basic Vector Information

- - Vector Name:
  - pUS23
- - Antibiotic Resistance:
  - Ampicillin
- - Length:
  - 4324 bp
- - Type:
  - Reporter vector
- - Replication origin:
  - ori
- - Source/Author:
  - Coleman NV, Holmes AJ.

pUS23 vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pUS23 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_45793        4324 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector pUS23, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Coleman NV, Holmes AJ.
  TITLE     The native Pseudomonas stutzeri strain Q chromosomal integron can 
            capture and express cassette-associated genes
  JOURNAL   Microbiology (Reading, Engl.) 151 (PT 6), 1853-1864 (2005)
  PUBMED    15941993
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Coleman NV, Holmes AJ.
  TITLE     Capture and expression of an aadB-gfp reporter plasmid by the 
            Pseudomonas stutzeri strain Q chromosomal integron
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4324)
  AUTHORS   Coleman NV, Holmes AJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2005) School of Molecular and Microbial 
            Biosciences, University of Sydney, Building G08, Sydney, NSW 2006, 
            Australia
REFERENCE   4  (bases 1 to 4324)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 4324)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology (Reading, Engl.) 151 (PT 6), 1853-1864 (2005)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (17-JAN-2005) School of Molecular and Microbial Biosciences, 
            University of Sydney, Building G08, Sydney, NSW 2006, Australia"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4324
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..33
                     /label=multiple cloning site
                     /note="multiple cloning site"
     terminator      38..124
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      216..243
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     misc_recomb     325..384
                     /label=aadB 59-base element (attC) recombination site
                     /note="aadB 59-base element (attC) recombination site"
     RBS             402..410
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      403..409
                     /gene="aadB"
                     /regulatory_class="ribosome_binding_site"
     CDS             415..945
                     /gene="aadB"
                     /label=aadB
                     /note="2''-aminoglycoside nucleotidyltransferase from 
                     Escherichia coli. Accession#: P0AE04"
     regulatory      955..961
                     /gene="gfpmut3"
                     /regulatory_class="ribosome_binding_site"
     CDS             968..1681
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     primer_bind     complement(1708..1724)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1732..1748)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1756..1786)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1801..1822)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2110..2698)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      complement(2722..2759)
                     /label=RNAII
                     /note="RNAII"
                     /regulatory_class="promoter"
     repeat_region   2722..2759
                     /label=Tn3 terminal inverted repeat
                     /note="Tn3 terminal inverted repeat"
     CDS             complement(2872..3729)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3730..3834)
                     /label=AmpR promoter
     primer_bind     4308..4324
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"

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