LOCUS       V002273                 4324 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002273
VERSION     V002273
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Coleman NV, Holmes AJ.
  TITLE     The native Pseudomonas stutzeri strain Q chromosomal integron can
            capture and express cassette-associated genes
  JOURNAL   Microbiology (Reading, Engl.) 151 (PT 6), 1853-1864 (2005)
   PUBMED   15941993
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Coleman NV, Holmes AJ.
  TITLE     Capture and expression of an aadB-gfp reporter plasmid by the
            Pseudomonas stutzeri strain Q chromosomal integron
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4324)
  AUTHORS   Coleman NV, Holmes AJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2005) School of Molecular and Microbial
            Biosciences, University of Sydney, Building G08, Sydney, NSW 2006,
            Australia
REFERENCE   4  (bases 1 to 4324)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 4324)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Microbiology (Reading, Engl.) 151 (PT 6), 1853-1864 (2005)"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (17-JAN-2005) School of Molecular and Microbial Biosciences,
            University of Sydney, Building G08, Sydney, NSW 2006, Australia"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4324
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..33
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     terminator      38..124
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      216..243
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     misc_recomb     325..384
                     /label="aadB 59-base element (attC) recombination site"
                     /note="aadB 59-base element (attC) recombination site"
     RBS             402..410
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      403..409
                     /gene="aadB"
                     /regulatory_class="ribosome_binding_site"
     CDS             415..945
                     /gene="aadB"
                     /label="2''-aminoglycoside nucleotidyltransferase"
                     /note="2''-aminoglycoside nucleotidyltransferase from
                     Escherichia coli. Accession#: P0AE04"
     regulatory      955..961
                     /gene="gfpmut3"
                     /regulatory_class="ribosome_binding_site"
     CDS             968..1681
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     primer_bind     complement(1708..1724)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1732..1748)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1756..1786)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1801..1822)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2110..2698)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     regulatory      complement(2722..2759)
                     /label="RNAII"
                     /note="RNAII"
                     /regulatory_class="promoter"
     repeat_region   2722..2759
                     /label="Tn3 terminal inverted repeat"
                     /note="Tn3 terminal inverted repeat"
     CDS             complement(2872..3729)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3730..3834)
                     /label="AmpR promoter"
     primer_bind     4308..4324
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 gaattcgagc tcggtacccg gggatcctct agataactaa ataaaacgaa aggctcagtc
       61 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac
      121 aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg
      181 acgcccgcca taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct
      241 ttttgcgttt ctacaaactc ttcctgtcgt catatctaca agccatccgt cgacttgcgt
      301 gccgctttca ggtcgcgata tgcggcctaa caattcgtcc aagccgacgc cgcttcgcgg
      361 cgcggcttaa ctcaggtgtt aggcattctg cagggcagat ctaaggaggt aggcatggac
      421 acaacgcagg tcacattgat acacaaaatt ctagctgcgg cagatgagcg aaatctgccg
      481 ctctggatcg gtgggggctg ggcgatcgat gcacggctag ggcgtgtaac acgcaagcac
      541 gatgatattg atctgacgtt tcccggcgag aggcgcggcg agctcgaggc aatagttgaa
      601 atgctcggcg ggcgcgtcat ggaggagttg gactatggat tcttagcgga gatcggggat
      661 gagttacttg actgcgaacc tgcttggtgg gcagacgaag cgtatgaaat cgcggaggct
      721 ccgcagggct cgtgcccaga ggcggctgag ggcgtcatcg ccgggcggcc agtccgttgt
      781 aacagctggg aggcgatcat ctgggattac ttttactatg ccgatgaagt accaccagtg
      841 gactggccta caaagcacat agagtcctac aggctcgcat gcacctcact cggggcggaa
      901 aaggttgagg tcttgcgtgc cgctttcagg tcgcgatatg cggcctaagg taccaaggag
      961 gttttttatg cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
     1021 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
     1081 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc
     1141 aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat
     1201 gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat
     1261 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac
     1321 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg
     1381 acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa
     1441 gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact
     1501 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
     1561 ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat
     1621 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa
     1681 atagggccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     1741 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     1801 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     1861 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     1921 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     1981 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     2041 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     2101 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     2161 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     2221 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     2281 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     2341 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     2401 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     2461 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     2521 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     2581 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     2641 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     2701 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     2761 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     2821 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     2881 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     2941 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     3001 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     3061 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     3121 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     3181 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     3241 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     3301 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     3361 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     3421 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     3481 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     3541 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     3601 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     3661 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     3721 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     3781 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     3841 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     3901 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     3961 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     4021 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     4081 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     4141 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
     4201 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     4261 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     4321 cagt
//
