pURYL vector (V002274)

MSDS

Basic Vector Information

- - Vector Name:
  - pURYL
- - Antibiotic Resistance:
  - Ampicillin
- - Length:
  - 5453 bp
- - Type:
  - Cloning vector
- - Replication origin:
  - ori
- - Source/Author:
  - Lv H, Gong Z.
- - Promoter:
  - SP6

pURYL vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pURYL vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_45788        5453 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pURYL, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Lv H, Gong Z.
  TITLE     Cost-effective and repeatable gene targeting in fission yeast using 
            a cloning-based and modular method
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   Lv H, Gong Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2012) School of Life Science, Fudan University, 
            Room 618, Science Building, Handan Road 220, Shanghai 200433, China
REFERENCE   3  (bases 1 to 5453)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5453)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-MAR-2012) School of Life Science, Fudan University, Room 618, 
            Science Building, Handan Road 220, Shanghai 200433, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        235..253
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             292..1002
                     /label=mEYFP
                     /note="enhanced YFP with monomerizing A206K mutation
                     (Zacharias et al., 2002)"
     CDS             1003..1020
                     /label=6xHis
                     /note="6xHis affinity tag"
     regulatory      1030..1522
                     /label=Saccharomyces cerevisiae Leu2 terminator
                     /note="Saccharomyces cerevisiae Leu2 terminator"
                     /regulatory_class="terminator"
     regulatory      1529..2050
                     /label=Schizosaccharomyces pombe Ura4 promoter
                     /note="Schizosaccharomyces pombe Ura4 promoter"
                     /regulatory_class="promoter"
     CDS             2051..2842
                     /label=S. pombe ura4
                     /note="orotidine 5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     regulatory      2846..3275
                     /label=Schizosaccharomyces pombe Ura4 terminator
                     /note="Schizosaccharomyces pombe Ura4 terminator"
                     /regulatory_class="terminator"
     promoter        complement(3342..3360)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3618..4206)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4380..5237)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5238..5342)
                     /label=AmpR promoter

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