Basic Vector Information
pURYL vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pURYL vector Sequence
LOCUS 40924_45788 5453 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pURYL, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5453) AUTHORS Lv H, Gong Z. TITLE Cost-effective and repeatable gene targeting in fission yeast using a cloning-based and modular method JOURNAL Unpublished REFERENCE 2 (bases 1 to 5453) AUTHORS Lv H, Gong Z. TITLE Direct Submission JOURNAL Submitted (24-MAR-2012) School of Life Science, Fudan University, Room 618, Science Building, Handan Road 220, Shanghai 200433, China REFERENCE 3 (bases 1 to 5453) TITLE Direct Submission REFERENCE 4 (bases 1 to 5453) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (24-MAR-2012) School of Life Science, Fudan University, Room 618, Science Building, Handan Road 220, Shanghai 200433, China" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..5453 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 235..253 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" CDS 292..1002 /label=mEYFP /note="enhanced YFP with monomerizing A206K mutation (Zacharias et al., 2002)" CDS 1003..1020 /label=6xHis /note="6xHis affinity tag" regulatory 1030..1522 /label=Saccharomyces cerevisiae Leu2 terminator /note="Saccharomyces cerevisiae Leu2 terminator" /regulatory_class="terminator" regulatory 1529..2050 /label=Schizosaccharomyces pombe Ura4 promoter /note="Schizosaccharomyces pombe Ura4 promoter" /regulatory_class="promoter" CDS 2051..2842 /label=S. pombe ura4 /note="orotidine 5'-phosphate decarboxylase, required for uracil biosynthesis" regulatory 2846..3275 /label=Schizosaccharomyces pombe Ura4 terminator /note="Schizosaccharomyces pombe Ura4 terminator" /regulatory_class="terminator" promoter complement(3342..3360) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" rep_origin complement(3618..4206) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4380..5237) /label=AmpR /note="beta-lactamase" promoter complement(5238..5342) /label=AmpR promoter
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